nucleotide sequences (Sangon Biotech)
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Nucleotide Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleotide+sequence/nucleotide+sequences/pmc13112984-49-10-41
Average 86 stars, based on 1 article reviews
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Synthesized:Article Title: Sensitive and Specific Analysis of miRNAs in Single Tumor-Derived Extracellular Vesicles Using CRISPR-Based Nanoflow Cytometry. Article Snippet: Tumor-derived extracellular vesicle (TEV) microRNAs (miRNAs) are promising cancer biomarkers but pose detection challenges due to their low abundance and sequence homology.. Here, we present a CRISPR/Cas13a-based nanoflow cytometry (nFCM) platform integrated with a DNA-guided orthogonal membrane fusion strategy for ultrasensitive miRNA detection of TEVs at the single particle level.. TEVs were identified with aptamers against CD63 and EpCAM markers to create an orthogonal barcodeanchored TEV (Orth-TEV). Article Title: Highly Sensitive Electrochemiluminescence Analysis of miRNA-107 Using AIE-Active Polymer Dots as Emitters. Article Snippet: Ultrapure water was acquired from a Millipore water purification system (≥18 MΩ·cm, Milli-Q, Millipore, Burlington, MA, USA). .. The Article Title: A Novel Genetic Engineering Approach for DON Detoxification Using a Yeast-Based Multi-Enzyme System Article Snippet: .. To ensure optimal protein expression in the yeast host, the Article Title: Highly Sensitive Electrochemiluminescence Analysis of miRNA-107 Using AIE-Active Polymer Dots as Emitters Article Snippet: Ultrapure water was acquired from a Millipore water purification system (≥18 MΩ⋅cm, Milli-Q, Millipore, Burlington, MA, USA). .. The Article Title: Field-deployable PAM/PFS-independent RspCas13d Platform for Rapid and Extraction-free Detection of ASFV and Its Gene-deleted Strains Article Snippet: ASFV causes high mortality and economic losses, and the emergence of gene-deleted strains highlights the need for rapid, sensitive, field-deployable diagnostics.. Here, we present an integrated diagnostic workflow termed LUCID–FLASH, which combines a rapid extraction-free lysis protocol (LUCID, Lysis Using Chemical Integration for Direct detection) with a single-step RAA–CRISPR/RspCas13d assay (FLASH, FLASH-deployable Lyophilized All-in-one Single-tube Hybrid-detection).. Together, these modules enable direct, instrument-free, and visual ASFV detection from swab samples within 30 min. We optimized and, for the first time, defined a chemical buffer formulation that supports high activities of both RAA and RspCas13d in a unified reaction. Expressing:Article Title: A Novel Genetic Engineering Approach for DON Detoxification Using a Yeast-Based Multi-Enzyme System Article Snippet: .. To ensure optimal protein expression in the yeast host, the other:Article Title: SinI and SinR function differently in biofilm formation, rhizosphere colonization, and biocontrol efficacy between Bacillus velezensis and B. subtilis Article Snippet: Article Title: SinI and SinR function differently in biofilm formation, rhizosphere colonization, and biocontrol efficacy between Bacillus velezensis and B. subtilis . Article Snippet: Saline:Article Title: Host-guest confinement in post-metallized COFs for enhanced electrochemiluminescence coupled with Y-shaped DNA amplification for microRNA-499 detection. Article Snippet: An ultrasensitive electrochemiluminescence (ECL) biosensor was developed for the detection of the acute myocardial infarction (AMI) biomarker miRNA-499 by synergistically integrating an iridium-metallized covalent organic framework (Ir@TpBpy) with enzyme-assisted Y-shaped DNA amplification.. The Ir@TpBpy COF was synthesized via post-metallization of a bipyridine-rich TpBpy framework with Ir(III) complexes.. This design significantly enhances the ECL performance not only through material loading but also via a synergistic optimization strategy: (1) the conjugated framework constructs continuous charge transport pathways to accelerate electron transfer; and (2) the porous architecture induces a guest confinement effect that locally enriches the coreactant triethylamine (TEA), facilitating radical kinetics. |
