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ATCC e coli o157 h7 sakai
Comparison of different broth media for short-term enrichment of <t> Escherichia coli </t> and Salmonella at 42°C a

E Coli O157 H7 Sakai, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleotide+multiple+sequence+alignment/Escherichia+coli+o157%3Ah7/pmc07301854-357-17-21
Average 96 stars, based on 999 article reviews

e coli o157 h7 sakai - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time Sequencing"

Article Title: Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time Sequencing

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00097-20

Figure Legend Snippet: Comparison of different broth media for short-term enrichment of Escherichia coli and Salmonella at 42°C a

Techniques Used: Comparison

The capture efficiency (CE) of immunomagnetic (IMS) beads for individual pure culture suspensions of Escherichia coli, Salmonella, and nontarget bacterial strains. CE was determined by dividing the viable count recovered after plating IMS beads by the initial count mixed with the beads.

Figure Legend Snippet: The capture efficiency (CE) of immunomagnetic (IMS) beads for individual pure culture suspensions of Escherichia coli, Salmonella, and nontarget bacterial strains. CE was determined by dividing the viable count recovered after plating IMS beads by the initial count mixed with the beads.

Techniques Used:

Taxonomic classification tree identification of Escherichia coli (A) and Salmonella (B), individually inoculated to wheat flour at a minimum abundance cut-off ratio of 3% generated by EPI2ME after 1 h of sequencing in a nanopore sequencer. Flour samples were inoculated with 0.1 log CFU/g E. coli O157:H7 or Salmonella Typhimurium strains, enriched in tryptic soy broth at 42°C, and subjected to immunomagnetic separation and multiple displacement amplification before sequencing.

Figure Legend Snippet: Taxonomic classification tree identification of Escherichia coli (A) and Salmonella (B), individually inoculated to wheat flour at a minimum abundance cut-off ratio of 3% generated by EPI2ME after 1 h of sequencing in a nanopore sequencer. Flour samples were inoculated with 0.1 log CFU/g E. coli O157:H7 or Salmonella Typhimurium strains, enriched in tryptic soy broth at 42°C, and subjected to immunomagnetic separation and multiple displacement amplification before sequencing.

Techniques Used: Generated, Sequencing, Immunomagnetic Separation, Multiple Displacement Amplification

Phylogenetic tree of wheat flour samples coinoculated with Escherichia coli and Salmonella with 0.1 CFU/g of each organism at a minimum abundance cut-off rate of 0.5% generated by EPI2ME after 1 h of sequencing in a nanopore sequencer. Flour samples were inoculated with 0.1 log CFU/g E. coli O157:H7 and Salmonella Typhimurium strains, enriched in tryptic soy broth at 42°C, and subjected to immunomagnetic separation and multiple displacement amplification before sequencing.

Figure Legend Snippet: Phylogenetic tree of wheat flour samples coinoculated with Escherichia coli and Salmonella with 0.1 CFU/g of each organism at a minimum abundance cut-off rate of 0.5% generated by EPI2ME after 1 h of sequencing in a nanopore sequencer. Flour samples were inoculated with 0.1 log CFU/g E. coli O157:H7 and Salmonella Typhimurium strains, enriched in tryptic soy broth at 42°C, and subjected to immunomagnetic separation and multiple displacement amplification before sequencing.

Techniques Used: Generated, Sequencing, Immunomagnetic Separation, Multiple Displacement Amplification

Phylogenetic clustering of inoculated flour samples inoculated at 0.1 log CFU/g, subjected to nanopore sequencing on a MinIon device in a quasimetagenomic approach. (A) Phylogenetic tree of Salmonella Typhimurium (ATCC 14028) and its tight clustering with the reference strain obtained from a 1-h sequencing run of Salmonella individual inoculation, subjected to analysis. (B) Phylogenetic tree of E. coli O157:H7 (ATCC 43895) and its tight clustering with the reference strain obtained from a 1-h sequencing run of E. coli and Salmonella dual inoculation, subjected to analysis. Numbers correspond to the closest GenBank strains for reference. Additional data are in Table S1 in the supplemental material.

Figure Legend Snippet: Phylogenetic clustering of inoculated flour samples inoculated at 0.1 log CFU/g, subjected to nanopore sequencing on a MinIon device in a quasimetagenomic approach. (A) Phylogenetic tree of Salmonella Typhimurium (ATCC 14028) and its tight clustering with the reference strain obtained from a 1-h sequencing run of Salmonella individual inoculation, subjected to analysis. (B) Phylogenetic tree of E. coli O157:H7 (ATCC 43895) and its tight clustering with the reference strain obtained from a 1-h sequencing run of E. coli and Salmonella dual inoculation, subjected to analysis. Numbers correspond to the closest GenBank strains for reference. Additional data are in Table S1 in the supplemental material.

Techniques Used: Nanopore Sequencing, Sequencing

Microbial abundance at the genus level for two different flour samples inoculated at 0.1 CFU/g with E. coli and Salmonella, respectively, resulting from nanopore sequencing using a Minion device followed by a conventional bioinformatics analysis pipeline. Additional data are in Table S2 in the supplemental material.

Figure Legend Snippet: Microbial abundance at the genus level for two different flour samples inoculated at 0.1 CFU/g with E. coli and Salmonella, respectively, resulting from nanopore sequencing using a Minion device followed by a conventional bioinformatics analysis pipeline. Additional data are in Table S2 in the supplemental material.

Techniques Used: Nanopore Sequencing

Figure Legend Snippet: Metagenomics analysis at different sequencing run times using conventional bioinformatics pipeline h

Techniques Used: Sequencing

Figure Legend Snippet: Metagenomics analysis at different sequencing run times using conventional bioinformatics pipeline h

Techniques Used: Sequencing

Activity Assay:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. The antibacterial activity of ZnO NPs synthesized using supernatants of AAB strains was tested both on E. coli O157:H7 ATCC 35,150 and S. aureus 6538P to represent both Gram-negative and Gram-positive bacteria, respectively. ..

Article Title: Characterization and Antimicrobial Activity Assessment of Postbiotic-Loaded Chia Mucilage-Montmorillonite Films Developed for Food Systems.
Article Snippet: .. The antibacterial activity of the postbiotics, as well as their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, was determined against Escherichia coli O157:H7 (ATCC 35150) and Listeria monocytogenes (ATCC 7644). ..

Synthesized:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. The antibacterial activity of ZnO NPs synthesized using supernatants of AAB strains was tested both on E. coli O157:H7 ATCC 35,150 and S. aureus 6538P to represent both Gram-negative and Gram-positive bacteria, respectively. ..

Bacteria:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. The antibacterial activity of ZnO NPs synthesized using supernatants of AAB strains was tested both on E. coli O157:H7 ATCC 35,150 and S. aureus 6538P to represent both Gram-negative and Gram-positive bacteria, respectively. ..

Concentration Assay:

Positive Control:

Article Title: Safety and Next-Generation Probiotic Potential of Mitsuokella multacida AGMB01124 Newly Isolated from Healthy Swine Feces.
Article Snippet: After differentiation, AGMB01124 were applied to the wells at concentrations of 107, 108, and 109 CFU/well in fresh Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum and incubated for 24 h. Cytotoxicity was assessed using the lactate dehydrogenase assay (EZLDH, DoGenBio, South Korea) on culture supernatants, according to the manufacturer’s protocol. .. Basal mediatreated cells were used as negative controls, and E. coli O157:H7 (ATCC 48395) served as the positive control for cytotoxic effects. ..

Cell Culture:

Article Title: A Feline Milk-Drived Pediococcus acidilactici M22 Alleviates Escherichia coli O157:H7 Infection Through Anti-Adhesion, Anti-Inflammation, and Microbiota Modulation.
Article Snippet: The strain was maintained in MRS broth (Haibo, Qingdao, China) and incubated at 37 ◦C under anaerobic conditions [12]. .. Escherichia coli O157:H7 (ATCC 43895) was obtained from the China General Microbiological Culture Collection Center (CGMCC, Beijing, China) and cultured in LB broth at 37 ◦C with constant shaking (200 rpm) [13]. ..

Isolation:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

UV-Vis Spectroscopy:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

Electron Microscopy:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

X-ray Diffraction:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

Fourier Transform Infrared Spectroscopy:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

Spectroscopy:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..

Produced:

Article Title: Thin films coating with ZnO nanoparticles synthesized via acetic acid bacteria: Structural and antibacterial characterization for food applications
Article Snippet: .. Specifically, this study aims to (i) synthesize ZnO NPs using AAB isolated from fermented foods, ii) create a thin film coating of ZnO NPs using spin coating techniques iii) characterize NPs and thin films through Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive analysis X-Ray (EDX), X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, and goniometer and iv) investigate the antimicrobial efficacy of ZnO NPs and thin films produced against Staphylococcus aureus 6538P and Escherichia coli O157:H7 ATCC 35150 to determine their potential as novel antimicrobial agents. ..