Review

nucleofector solution (Amaxa)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Amaxa nucleofector solution
Nucleofector Solution, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofector+solution/nucleofector+solution+v/pm41307113-291-13-15
Average 86 stars, based on 1 article reviews

nucleofector solution - by Bioz Stars, 2026-10

86/100 stars

Images

Transfection:

Article Title: Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode.
Article Snippet: .. RAW cells were transfected by electroporation in Amaxa Nucleofector Solution V using the T-020 setting on an Amaxa Nucleofector 2b device. ..

Article Title: Discovery of novel murine PML isoforms
Article Snippet: Pml −/− MEFs were transfected with individual mPML isoforms using Cell Line Nucleofector Kit V (Lonza, Cat. No. VCA-1003). .. Briefly, cell pellets containing 4 × 10 6 cells were resuspended in 100 μl of Amaxa nucleofector solution, mixed with 5 μg of plasmid DNA, and transfected using Amaxa Nucleofector II (Lonza) with T020 pre-programmed settings. .. After the pulse, the cells were mixed with 500 μl of RPMI-1640 medium (Merck, Cat. No. R8758), incubated for 15 min at 37°C in humidified atmosphere with 5% CO 2 , and seeded on cover slips or 6-well plates.

Electroporation:

Plasmid Preparation:

other:

Article Title: Uses of labeled HSP90 inhibitors
Article Snippet: A portion of the lesion is taken for fresh tissue sectioning performed on a Vibratome (Leica VT1000).

Injection:

Article Title: A genetically encoded marker for imaging membranes of Plasmodium
Article Snippet: Schizonts were obtained by culturing 500 μl blood containing > 1% parasitemia in schizont medium (RPMI-1640, gibco) supplemented with 20% FCS (gibco) and 1 μg/ml gentamycin (PAA) for about 20 h at 37 °C and were purified over a 55% Nycodenz (Axis-shield diagnostics) gradient. .. DNA and schizonts were mixed with 100 μl Nucleofector solution from the human T cell Nucleofector Kit, electroporated using the Amaxa Nucleofector II device (Lonza), and immediately injected intravenously into a mouse. .. Transgenic parasites were selected by administering pyrimethamine (7 μg/ml, Sigma-Aldrich) to the drinking water one day after transfection.