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Amaxa dermal fibroblasts nucleofector kit
Dermal Fibroblasts Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofector+kit/dermal+fibroblasts+kit+nucleofector/pm42314933-260-18-22
Average 86 stars, based on 1 article reviews
dermal fibroblasts nucleofector kit - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Fibroblast-Specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Diminishing Collagenous Matrix.
Article Snippet: To assess loading consistency, membranes were stripped and reprobed with an anti-β-actin antibody (Sigma-Aldrich).

Transfection:

Article Title: Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells.
Article Snippet: .. Human fibroblasts were transfected with the human dermal fibroblast Nucleofector Kit (Amaxa GmbH, Cologne, Germany) according to manufacturer’s instructions. ..

Article Title: Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Article Snippet: To assess loading consistency, membranes were stripped and reprobed with an anti-β-actin antibody (Sigma-Aldrich). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD).

Article Title: Trichothiodystrophy-causative pathogenic variants impair a cooperative action of TFIIH and DDX1 in R-loop processing
Article Snippet: .. For transfection experiments, 10 6 fibroblasts were resuspended in 100 ml Human Dermal Fibroblast Nucleofector solution (VPD1001-Amaxa) mixed with 5 μg of RNase H1 GFP expressing plasmid DNA ( NM_002936 , Origene), electroporated according to Nucleofector A-20 or U-05 programs, seeded and lysed 48 h later. ..

Article Title: Notch signalling regulates fibroblast activation and collagen release in systemic sclerosis.
Article Snippet: 21 22 Activation of the (Sigma-Aldrich, Steinheim, Germany) at concentrations of 1.0 and 10 μmol/l for 24 h. Jag-1 was added 1 h after DAPT at a concentration of 5.0 μg/ml. .. In addition to DAPT, dermal SSc fi broblasts were transfected with 1.5 μg siRNA against Notch-1 using the human dermal fi broblast nucleofector kit (Amaxa, Cologne, Germany) (supplementary table 2). .. 14 Fibroblasts transfected with control siRNA (Ambion, Darmstadt, Germany) served as controls.

Mutagenesis:

Article Title: Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Article Snippet: To assess loading consistency, membranes were stripped and reprobed with an anti-β-actin antibody (Sigma-Aldrich). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD).

Plasmid Preparation:

Article Title: Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Article Snippet: To assess loading consistency, membranes were stripped and reprobed with an anti-β-actin antibody (Sigma-Aldrich). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD).

Article Title: Trichothiodystrophy-causative pathogenic variants impair a cooperative action of TFIIH and DDX1 in R-loop processing
Article Snippet: .. For transfection experiments, 10 6 fibroblasts were resuspended in 100 ml Human Dermal Fibroblast Nucleofector solution (VPD1001-Amaxa) mixed with 5 μg of RNase H1 GFP expressing plasmid DNA ( NM_002936 , Origene), electroporated according to Nucleofector A-20 or U-05 programs, seeded and lysed 48 h later. ..

Electroporation:

Article Title: Fibroblast-specific Deletion of Yap/Taz Impairs Mouse Postnatal Dermal Development by Suppressing Collagen Production and Deposition
Article Snippet: To assess loading consistency, membranes were stripped and reprobed with an anti-β-actin antibody (Sigma-Aldrich). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD). .. Mouse dermal fibroblasts were transiently transfected with a YAP/TAZ mutant plasmid ( ) by electroporation using dermal fibroblasts nucleofector kit (Amaxa Biosystems, Gaithersburg, MD).

Expressing:

Article Title: Trichothiodystrophy-causative pathogenic variants impair a cooperative action of TFIIH and DDX1 in R-loop processing
Article Snippet: .. For transfection experiments, 10 6 fibroblasts were resuspended in 100 ml Human Dermal Fibroblast Nucleofector solution (VPD1001-Amaxa) mixed with 5 μg of RNase H1 GFP expressing plasmid DNA ( NM_002936 , Origene), electroporated according to Nucleofector A-20 or U-05 programs, seeded and lysed 48 h later. ..



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