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marker 4  (Beyotime)


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    Structured Review

    Beyotime marker 4
    Marker 4, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+marker+dapi/DAPI/pmc11033159-239-19-22
    Average 99 stars, based on 32727 article reviews
    marker 4 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Staining:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.



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    Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. <t>Approximate</t> <t>molecular</t> weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with <t>DAPI</t> staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)
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    Image Search Results


    Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Integrated Mendelian Randomization and Single‐Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy

    doi: 10.1111/cns.70172

    Figure Lengend Snippet: Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)

    Article Snippet: Subsequently, slices were briefly rinsed in PBS for 5 min and stained with the DAPI nuclear marker (# D1306, 1:10000, Molecular Probes) for 5 min. After a final PBS rinse, slices were mounted on gel‐coated slides, cover‐slipped with Prolong gold anti‐fade (# P36930, Vector Laboratories), and stored at 4°C until further analysis.

    Techniques: Expressing, Animal Model, Injection, Extraction, Western Blot, Control, Immunofluorescence, Staining, Fluorescence