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nuclear binding dye 4 0,6-diamidino-2-phenylindole dihydrochloride (dapi)  (Carl Zeiss)


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    Carl Zeiss nuclear binding dye 4 0,6-diamidino-2-phenylindole dihydrochloride (dapi)
    Nuclear Binding Dye 4 0,6 Diamidino 2 Phenylindole Dihydrochloride (Dapi), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+binding+dye+dapi/2+4+6+diamidino+phenylindole/pm15710218-71-18-28
    Average 90 stars, based on 1 article reviews
    nuclear binding dye 4 0,6-diamidino-2-phenylindole dihydrochloride (dapi) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: An Epilepsy-Associated CILK1 Variant Compromises KATNIP Regulation and Impairs Primary Cilia and Hedgehog Signaling
    Article Snippet: After multiple rinses, slides were mounted in antifade reagent containing DAPI (4′,6-diamidino-2-phenylindole) for imaging via a confocal Laser Scanning Microscopy 700 from ZEISS (Chester, VA, USA) at the UVA Advanced Microscopy Facility.

    Article Title: Developmental dynamics of marmoset prefrontal cortical SST and PV interneuron networks highlight primate-specific features
    Article Snippet: The objectives used were Zeiss EC-Plan-Neofluar 5×0.16 (420330-9901), EC-Plan-Neofluar 10×0.3 (420340-9901) and Plan Apochromat 20×0.8 (420650-9901).

    Article Title: Vertical distribution and light energy capture by AAP bacteria along contrasted areas of productivity in the Atlantic Ocean
    Article Snippet: Cells were stained with 4’6-diamidino-2-phenylindole (DAPI) and counted using an epifluorescence Zeiss Axio Imager.D2 microscope equipped with a Plan-Apochromat 63×/1.46 Oil Corr objective and a Collibri LED module illumination system (Carl Zeiss, Jena, Germany).

    Article Title: Distinctive physiology of molecularly identified medium spiny neurons in the macaque putamen.
    Article Snippet: 4,6-diamidino-2-phenylindole (DAPI) stain was also used to identify cortical layers.70 Slices were mounted and imaged on an upright ZEISS Axio Imager Z2 microscope (Carl Zeiss AG, Jena, Germany) equipped with an Axiocam 506 monochrome camera and 0.63x optivar.

    Article Title: Cell wall composition in Cryptococcus neoformans is media dependent and alters host response, inducing protective immunity.
    Article Snippet: For microscopy, WGA was detected using the Green Fluorescent Protein (GFP) filter settings, and CFW was detected using the 496-diamidino-2-phenylindole (DAPI) settings in a Zeiss Axio Imager M2 equipped with a Hamamatsu F la sh4 .0 Complementa ry Meta l -Ox ide - Semiconductor (CMOS) camera.

    Article Title: Chemical Hypoxia Induces Pyroptosis in Neuronal Cells by Caspase-Dependent Gasdermin Activation
    Article Snippet: Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) and stained cells were observed under a Zeiss LSM-800 confocal microscope (Zeiss, Oberkochen, Germany).

    Article Title: Taxonomy of the genus Poterioochromonas (Chrysophyceae) based on morphological and molecular evidence
    Article Snippet: The 4′, 6‐diamidino‐2‐phenylindole (DAPI) filter and HPV 120 V (Carl Zeiss) were used for fluorescence microscopic observation of stained samples.

    Fluorescence:

    Article Title: Milk‐derived extracellular vesicles functionalized with anti‐tumour necrosis factor‐α nanobody and anti‐microbial peptide alleviate ulcerative colitis in mice
    Article Snippet: For the staining of macrophages, 8 μm cryosections were fixed with 4% paraformaldehyde and blocked with PBS buffer containing 5% BSA, followed by staining with rabbit primary macrosialin (CD68) antibody (1:200, ab76308, Abcam, UK) and then washed and incubated with AlexaFluor594‐conjugated goat anti‐rabbit IgG (1:200, A‐11037, Thermofisher, USA). .. Nuclei were counterstained with 4,6‐diamino‐2‐phenyl indole (DAPI) and samples were analysed by fluorescence confocal microscopy (LSM700, Zeiss, Germany). .. For Haematoxylin and Eosin (H&E) staining, the proximal and distal colon, liver, spleen and kidney fixed in 4% paraformaldehyde were embedded in paraffin and cut into 5 μm thick slices and subjected to H&E (C0105) and alcian blue staining as per manufacturer's instructions (C0155, Beyotime, China).

    Confocal Microscopy:

    Article Title: Milk‐derived extracellular vesicles functionalized with anti‐tumour necrosis factor‐α nanobody and anti‐microbial peptide alleviate ulcerative colitis in mice
    Article Snippet: For the staining of macrophages, 8 μm cryosections were fixed with 4% paraformaldehyde and blocked with PBS buffer containing 5% BSA, followed by staining with rabbit primary macrosialin (CD68) antibody (1:200, ab76308, Abcam, UK) and then washed and incubated with AlexaFluor594‐conjugated goat anti‐rabbit IgG (1:200, A‐11037, Thermofisher, USA). .. Nuclei were counterstained with 4,6‐diamino‐2‐phenyl indole (DAPI) and samples were analysed by fluorescence confocal microscopy (LSM700, Zeiss, Germany). .. For Haematoxylin and Eosin (H&E) staining, the proximal and distal colon, liver, spleen and kidney fixed in 4% paraformaldehyde were embedded in paraffin and cut into 5 μm thick slices and subjected to H&E (C0105) and alcian blue staining as per manufacturer's instructions (C0155, Beyotime, China).



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