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Shanghai DingGuo Biotech Co np-40 lysis buffer
Np 40 Lysis Buffer, supplied by Shanghai DingGuo Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/np40+buffer/np+40+lysis+buffer/ppr0597142-39-36-42
Average 90 stars, based on 1 article reviews
np-40 lysis buffer - by Bioz Stars, 2026-09
90/100 stars

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Over Expression:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.

Lysis:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.

Marker:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.

Chromatin Immunoprecipitation:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.

Real-time Polymerase Chain Reaction:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.

Bicinchoninic Acid Protein Assay:

Article Title: ZBTB5 enhances the resistance of cervical cancer to paclitaxel by regulating BCL6
Article Snippet: Materials also included Dulbecco’s modi ed Eagle’s medium (DMEM) (HyClone, USA), fetal bovine serum (Evergreen, Hangzhou, China), PTX (Perilla frutescens, Beijing Union Pharmaceutical Factory, Lot number: 141101), ZBTB5 overexpression and ZBTB5 sgRNA lentiviruses (Shanghai Genechem, China), RIPA lysis buffer, NP-40 lysis buffer (Shanghai Dingguo Biotechnology), prestained protein marker (Thermo, USA), Apoptosis kit (eBioscienceTM Annexin V USA), paraformaldehyde (Sigma, USA), ChIP kit (Pierce, USA), qPCR kit (Promega, USA), BCA protein assay kit (Beyotime, China), ANTI-FLAG® M2 A nity Gel (Sigma, USA), 3X FLAG peptide (Sigma, USA), ECL-PLUS/kit (Thermo, USA), X-ray lm developer powder (Shanghai Guanlong Photographic Materials Company, China), iodoacetamide (Sigma, USA), dithiothreitol (Sigma, USA), methanol, formic acid (Thermo, USA), and ultra ltration centrifuge tubes (Sartorius, USA).

Article Title: The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
Article Snippet: RIPA lysate (WB-0071) and NP-40 lysate (P0013F) were purchased from Shanghai Dingguo Biotechnology Co. Prestained protein markers (00161543) and an ECL-PLUS/Kit (M3121/1859022) were provided by Thermo.



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