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np40 lysis buffer  (MedChemExpress)


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    Structured Review

    MedChemExpress np40 lysis buffer
    Np40 Lysis Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/np40+buffer/NP-40+Lysis+Buffer/pm42012885-268-14-27
    Average 97 stars, based on 129 article reviews
    np40 lysis buffer - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Co-Immunoprecipitation Assay:

    Article Title: UGGT1 mediates N-glycosylation of LGALS3BP to induce angiogenesis in liver cancer via the NOTCH signaling pathway
    Article Snippet: Cells were co-cultured for 48 h in DMEM containing 5% 186 FBS (Procell, China) before HUVECs were harvested for subsequent tube formation assays. .. 187 2.11 Co-Immunoprecipitation (Co-IP) 188 Huh7 cells were lysed on ice for 40 min using a mild NP-40–based lysis buffer (MCE, 189 USA). .. After centrifugation, the supernatant of the lysate was divided into two groups: the 190 experimental group was treated with specific antibodies against LGALS3BP (10281-1-AP, 191 Proteintech, China) or UGGT1 (ab241357, Abcam, UK); the control group was treated with an 192 equivalent amount of rabbit anti-IgG (ab172730, Abcam, UK).

    Lysis:

    Article Title: UGGT1 mediates N-glycosylation of LGALS3BP to induce angiogenesis in liver cancer via the NOTCH signaling pathway
    Article Snippet: Cells were co-cultured for 48 h in DMEM containing 5% 186 FBS (Procell, China) before HUVECs were harvested for subsequent tube formation assays. .. 187 2.11 Co-Immunoprecipitation (Co-IP) 188 Huh7 cells were lysed on ice for 40 min using a mild NP-40–based lysis buffer (MCE, 189 USA). .. After centrifugation, the supernatant of the lysate was divided into two groups: the 190 experimental group was treated with specific antibodies against LGALS3BP (10281-1-AP, 191 Proteintech, China) or UGGT1 (ab241357, Abcam, UK); the control group was treated with an 192 equivalent amount of rabbit anti-IgG (ab172730, Abcam, UK).

    Article Title: Bisphenol A exacerbates chronic non-bacterial prostatitis by upregulating ITGAX and promoting M1 macrophage polarization
    Article Snippet: Chronic non-bacterial prostatitis (CNP) is a common urological disease, whereas the effects of bisphenol A (BPA) on CNP remain unclear.. We systematically evaluated the associations between BPA and CNP by combining dietary exposure assessment, urinary BPA detection, and animal model validation, and performed integrated multi‐omics analyses to decipher the underlying molecular mechanisms.. Dietary questionnaire data from 1,256 individuals showed that BPA exposure was associated with increased CNP risk (OR = 1.46, 95% CI: 1.08–1.98), particularly among individuals with BMI < 24 kg/m2.

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: Dietary myo-inositol exerts pleiotropic effects on T cell activation, antioxidant defense and fatty acid metabolism to enhance anti-bacterial immunity in tilapia.
    Article Snippet: Bacterial diseases cause major economic losses in aquaculture, underscoring the need for feed additives that enhance fish immunity.. Myo-inositol (MI), an essential nutrient for fish growth, possesses antioxidant and lipidmetabolism modulating properties.. However, the role of MI in regulating T cell function remains unclear.

    Article Title: An orally bioavailable pan-αv/α5β1 integrin antagonist prevents aggressive prostate cancer progression via suppressing both oncogenic signals and CD47-mediated immune escape.
    Article Snippet: .. Cells were collected and lysed in NP-40 lysis buffer (50 mM Tris-HCl pH 7.4, 10% glycerol, 50 mM NaCl, 0.5% sodium deoxycholate, 1% NP-40, 20 mM NaF) supplemented with 1 mM PMSF and protease inhibitor cocktail (MedChemExpress, China), subjected to two freeze-thaw cycles using liquid nitrogen and a 37°C water bath. ..

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: .. For immunoprecipitation (IP), magnetic beads were washed three times with NP-40 lysis buffer, then incubated overnight at 4°C with primary antibodies (HY-K0205,MCE). ..

    Transfection:

    Article Title: Parabacteroides goldsteinii and its metabolite 7-KLCA attenuate endometriosis via TGR5 to reprogram macrophages by modulating the PPARγ/GPR132 axis
    Article Snippet: HEK 293 T cells were transfected with the TGR5-Flag plasmids using Lipofectamine 3000 (L3000001, Thermo Fisher, USA). .. Following transfection, the cells were lysed with NP-40 buffer (HY-Y1884, MCE, USA) and subjected to three freeze-thaw cycles in liquid nitrogen. ..

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Protease Inhibitor:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: Dietary myo-inositol exerts pleiotropic effects on T cell activation, antioxidant defense and fatty acid metabolism to enhance anti-bacterial immunity in tilapia.
    Article Snippet: Bacterial diseases cause major economic losses in aquaculture, underscoring the need for feed additives that enhance fish immunity.. Myo-inositol (MI), an essential nutrient for fish growth, possesses antioxidant and lipidmetabolism modulating properties.. However, the role of MI in regulating T cell function remains unclear.

    Article Title: An orally bioavailable pan-αv/α5β1 integrin antagonist prevents aggressive prostate cancer progression via suppressing both oncogenic signals and CD47-mediated immune escape.
    Article Snippet: .. Cells were collected and lysed in NP-40 lysis buffer (50 mM Tris-HCl pH 7.4, 10% glycerol, 50 mM NaCl, 0.5% sodium deoxycholate, 1% NP-40, 20 mM NaF) supplemented with 1 mM PMSF and protease inhibitor cocktail (MedChemExpress, China), subjected to two freeze-thaw cycles using liquid nitrogen and a 37°C water bath. ..

    Magnetic Beads:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: .. For immunoprecipitation (IP), magnetic beads were washed three times with NP-40 lysis buffer, then incubated overnight at 4°C with primary antibodies (HY-K0205,MCE). ..

    cDNA Synthesis:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Real-time Polymerase Chain Reaction:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    SYBR Green Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    CCK-8 Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Multiple Displacement Amplification:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Protein Extraction:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Bicinchoninic Acid Protein Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Immunoprecipitation:

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: .. For immunoprecipitation (IP), magnetic beads were washed three times with NP-40 lysis buffer, then incubated overnight at 4°C with primary antibodies (HY-K0205,MCE). ..

    Incubation:

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: .. For immunoprecipitation (IP), magnetic beads were washed three times with NP-40 lysis buffer, then incubated overnight at 4°C with primary antibodies (HY-K0205,MCE). ..



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