Review



tissue microarrays  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Novus Biologicals tissue microarrays
    Expression of SORT1 in ovarian healthy and tumoral tissues. Immunohistochemical staining of SORT1 in tissue <t>microarrays</t> (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in ovarian tumors. With the immunohistochemical (IHC) scoring system used, the level of expressed SORT1 ranged from 0 to a maximum of 12. ( A ) Representative SORT1 staining by IHC in ovarian healthy, primary and metastases biopsies. Nuclei are stained blue with hematoxylin. Arrows point to ovarian healthy epithelia. Black bars represent 50 µm. ( B ) IHC scores from healthy tissues ( n = 20), benign tumors ( n = 18), malignant tumors ( n = 45) and metastases ( n = 10). ( C ) RT-qPCR was used to quantify the transcript levels of SORT1 in healthy and cancerous ovarian tissues. The SORT1 transcript levels in tumor samples were segregated according to tumor grade and then compared to the values from healthy ovarian tissues. n = 8 for healthy and for Grade I tumor samples, n = 9 for Grade II, n = 17 for Grade III and n = 7 for Grade IV tumor samples. ( D ) The mean IHC scores for SORT1 is shown for healthy ovarian tissue ( n = 20), benign tumors ( n = 18), LGSC ( n = 21), HGSC ( n = 6), CCC ( n = 5), MC ( n = 3), EC ( n = 13), TCC ( n = 5) and GC and NE ( n = 20). All scatter plots include lines showing the means ± SEM, and where each point represents an individual tissue sample.
    Tissue Microarrays, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Multi-Organ+Tissue+MicroArray+(Normal)/pmc09031804-76-1-19
    Average 93 stars, based on 5 article reviews
    tissue microarrays - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers"

    Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers

    Journal: Cancers

    doi: 10.3390/cancers14081877

    Expression of SORT1 in ovarian healthy and tumoral tissues. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in ovarian tumors. With the immunohistochemical (IHC) scoring system used, the level of expressed SORT1 ranged from 0 to a maximum of 12. ( A ) Representative SORT1 staining by IHC in ovarian healthy, primary and metastases biopsies. Nuclei are stained blue with hematoxylin. Arrows point to ovarian healthy epithelia. Black bars represent 50 µm. ( B ) IHC scores from healthy tissues ( n = 20), benign tumors ( n = 18), malignant tumors ( n = 45) and metastases ( n = 10). ( C ) RT-qPCR was used to quantify the transcript levels of SORT1 in healthy and cancerous ovarian tissues. The SORT1 transcript levels in tumor samples were segregated according to tumor grade and then compared to the values from healthy ovarian tissues. n = 8 for healthy and for Grade I tumor samples, n = 9 for Grade II, n = 17 for Grade III and n = 7 for Grade IV tumor samples. ( D ) The mean IHC scores for SORT1 is shown for healthy ovarian tissue ( n = 20), benign tumors ( n = 18), LGSC ( n = 21), HGSC ( n = 6), CCC ( n = 5), MC ( n = 3), EC ( n = 13), TCC ( n = 5) and GC and NE ( n = 20). All scatter plots include lines showing the means ± SEM, and where each point represents an individual tissue sample.
    Figure Legend Snippet: Expression of SORT1 in ovarian healthy and tumoral tissues. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in ovarian tumors. With the immunohistochemical (IHC) scoring system used, the level of expressed SORT1 ranged from 0 to a maximum of 12. ( A ) Representative SORT1 staining by IHC in ovarian healthy, primary and metastases biopsies. Nuclei are stained blue with hematoxylin. Arrows point to ovarian healthy epithelia. Black bars represent 50 µm. ( B ) IHC scores from healthy tissues ( n = 20), benign tumors ( n = 18), malignant tumors ( n = 45) and metastases ( n = 10). ( C ) RT-qPCR was used to quantify the transcript levels of SORT1 in healthy and cancerous ovarian tissues. The SORT1 transcript levels in tumor samples were segregated according to tumor grade and then compared to the values from healthy ovarian tissues. n = 8 for healthy and for Grade I tumor samples, n = 9 for Grade II, n = 17 for Grade III and n = 7 for Grade IV tumor samples. ( D ) The mean IHC scores for SORT1 is shown for healthy ovarian tissue ( n = 20), benign tumors ( n = 18), LGSC ( n = 21), HGSC ( n = 6), CCC ( n = 5), MC ( n = 3), EC ( n = 13), TCC ( n = 5) and GC and NE ( n = 20). All scatter plots include lines showing the means ± SEM, and where each point represents an individual tissue sample.

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR

    Expression of SORT1 in endometrial healthy tissues and endometrial tumor biopsies. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in endometrial tumors. ( A ) Representative SORT1 staining by IHC in endometrial healthy tissue and endometrial tumor biopsies. Nuclei are stained blue with hematoxylin. Black bars represent 50 µm. ( B ) SORT1 IHC scores between healthy endometrial tissues ( n = 2) and endometrial cancerous tissues ( n = 12). Scatter plot include lines showing the means ± SEM, and where each point represents an individual tissue sample.
    Figure Legend Snippet: Expression of SORT1 in endometrial healthy tissues and endometrial tumor biopsies. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in endometrial tumors. ( A ) Representative SORT1 staining by IHC in endometrial healthy tissue and endometrial tumor biopsies. Nuclei are stained blue with hematoxylin. Black bars represent 50 µm. ( B ) SORT1 IHC scores between healthy endometrial tissues ( n = 2) and endometrial cancerous tissues ( n = 12). Scatter plot include lines showing the means ± SEM, and where each point represents an individual tissue sample.

    Techniques Used: Expressing, Immunohistochemical staining, Staining

    Related Articles

    Expressing:

    Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers
    Article Snippet: The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).. The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).. Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).

    Immunohistochemical staining:

    Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers
    Article Snippet: The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).. The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).. Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).

    Staining:

    Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers
    Article Snippet: The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).. The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).. Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).

    Quantitative RT-PCR:

    Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers
    Article Snippet: The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).The siRNAs against a random scrambled sequence (siSCR; AllStar Negative Control siRNA, 1027281) and SORT1 (siSORT1; Hs_SORT_4 FlexiTube siRNA: SI00073640) were obtained from Qiagen (Toronto, ON, Canada).. The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).. Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).Respective and detailed clinical data of the tissue samples used can be found at the following vendors’ web site ( https://www.novusbio.com/products/multi-organ-tissue-micro-array_nbp2-30170 ; https:// www.novusbio.com/products/ovary-tissue-micro-array_nbp2-30290 ; https:// www.novusbio.com/products/various-tissue-micro-array_nbp2-30330 ; all web sites accessed on 5 April 2022).



    Similar Products

    normal brain tissue microarrays  (Biomax Inc)
    86
    Biomax Inc normal brain tissue microarrays
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Normal Brain Tissue Microarrays, supplied by Biomax Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/microarrays+tissue/pmc13052156-395-6-11
    Average 86 stars, based on 1 article reviews
    normal brain tissue microarrays - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    cancer research icr mice  (Novus Biologicals)
    94
    Novus Biologicals cancer research icr mice
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Cancer Research Icr Mice, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Mouse+Multi-Organ+Tissue+MicroArray+(Normal)/bio_rxiv__64898__2026__02__27__708441-22-14-22
    Average 94 stars, based on 1 article reviews
    cancer research icr mice - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    nbp2  (Novus Biologicals)
    94
    Novus Biologicals nbp2
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Multi-tissue+Tissue+MicroArray+(Normal)/pm41579862-796-162-159
    Average 94 stars, based on 1 article reviews
    nbp2 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    ffpe human normal tissue microarray nbp2 78113 novus biologicals  (Novus Biologicals)
    94
    Novus Biologicals ffpe human normal tissue microarray nbp2 78113 novus biologicals
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Ffpe Human Normal Tissue Microarray Nbp2 78113 Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Multi-tissue+Tissue+MicroArray+(Normal)/pm41579862-999-14-19
    Average 94 stars, based on 1 article reviews
    ffpe human normal tissue microarray nbp2 78113 novus biologicals - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    nbp2 78113 tma  (Novus Biologicals)
    94
    Novus Biologicals nbp2 78113 tma
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Nbp2 78113 Tma, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Multi-tissue+Tissue+MicroArray+(Normal)/pm41406940-214-147-144
    Average 94 stars, based on 1 article reviews
    nbp2 78113 tma - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    normal tissue microarray  (Novus Biologicals)
    93
    Novus Biologicals normal tissue microarray
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Normal Tissue Microarray, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Normal+Tissue+MicroArray+(Normal)/pm41406940-220-2-13
    Average 93 stars, based on 1 article reviews
    normal tissue microarray - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human multi tissue tissue microarray  (Novus Biologicals)
    94
    Novus Biologicals human multi tissue tissue microarray
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Human Multi Tissue Tissue Microarray, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Multi-tissue+Tissue+MicroArray+(Normal)/pmc12765945-19-0-6
    Average 94 stars, based on 1 article reviews
    human multi tissue tissue microarray - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    human brain tissue  (Novus Biologicals)
    93
    Novus Biologicals human brain tissue
    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue <t>microarrays</t> with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.
    Human Brain Tissue, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Brain+Tissue+MicroArray+(Normal)/pmc12826922-58-5-10
    Average 93 stars, based on 1 article reviews
    human brain tissue - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Inhibition of cell surface GRP78 on brain tumors reverses drug resistance and stops cancer stem cell expansion

    doi: 10.1016/j.jbc.2026.111146

    Figure Lengend Snippet: Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Article Snippet: Briefly, three human brain tumors and normal brain tissue microarrays (T174c; Biomax. us) were deparaffinized with xylene and washed with PBS.

    Techniques: Staining, Microarray