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adult normal human brain tissue lysates  (Novus Biologicals)


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    Novus Biologicals adult normal human brain tissue lysates
    a Comparison of CHSY1 gene expression in glioma subtypes and <t>normal</t> <t>brain</t> <t>tissue</t> in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal <t>human</t> brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.
    Adult Normal Human Brain Tissue Lysates, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Human+Brain+Tissue+MicroArray+(Normal)/pmc07000683-223-0-9
    Average 93 stars, based on 2 article reviews
    adult normal human brain tissue lysates - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability"

    Article Title: Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability

    Journal: Oncogenesis

    doi: 10.1038/s41389-020-0197-0

    a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.
    Figure Legend Snippet: a Comparison of CHSY1 gene expression in glioma subtypes and normal brain tissue in the REMBRANDT glioma microarray database. ** P < 0.01, **** P < 0.0001. b High expression of CHSY1 was associated with worse overall survival in glioma patients. The high and low expression groups were divided by median expression level of CHSY1 in 329 cases. These data were from the REMBRANDT database ( http://www.betastasis.com/glioma/rembrandt/ ). c Immunohistochemistry of CHSY1 (upper panel) and CS56 (lower panel) on tissue array contains 85 primary glioma cases. The staining was visualized in brown color with a 3,3-diaminobenzidine liquid substrate system. All sections were counterstained with hematoxylin. Representative images of four glioma cases with different staining intensities are shown. Amplified images are shown at the bottom right of each image. Scale bars, 50 μm. Arrows indicate positive stained glioma cells. d Representative images of CHSY1 staining on normal brain tissue ( n = 5). e Statistical analysis of immunohistochemistry in glioma tissue array. Mann–Whitney U -test was used. P -values are shown at top. f Expression of CHSY1 in glioma cell lines and normal human brain tissue. The protein expression was analyzed by western blotting. Total loading protein is shown at bottom. Relative expression levels to total brain tissue form three independent blots are shown at the right.

    Techniques Used: Comparison, Gene Expression, Microarray, Expressing, Immunohistochemistry, Staining, Amplification, MANN-WHITNEY, Western Blot

    Related Articles

    In Situ Hybridization:

    Article Title: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296). .. Chromogenic double in situ hybridization was performed for the human brain tissue using the RNAscope 2.5 HD Duplex Assay (Advanced Cell Diagnostics) according to the manufacturer's protocol. .. A Fluorescent Multiplex assay was used for the human retina tissue according to the manufacturer's protocol (Advanced Cell Diagnostics).

    RNAscope:

    Article Title: FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296). .. Chromogenic double in situ hybridization was performed for the human brain tissue using the RNAscope 2.5 HD Duplex Assay (Advanced Cell Diagnostics) according to the manufacturer's protocol. .. A Fluorescent Multiplex assay was used for the human retina tissue according to the manufacturer's protocol (Advanced Cell Diagnostics).

    Article Title: Transcriptomic analysis of the human habenula in schizophrenia
    Article Snippet: .. smFISH experiments were performed on postmortem human brain tissue using RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) and 4-Plex Ancillary Kit as previously described . ..

    Labeling:

    Article Title: Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease
    Article Snippet: .. In human brain tissue, the goat antiserum sc-1784 and the mouse monoclonal antibody MAB2791 (R&D Systems) demonstrated distinct neuronal CCL2 labeling comparable to the staining pattern observed in mouse brain. ..

    Staining:

    Article Title: Isoglutaminyl cyclase contributes to CCL2-driven neuroinflammation in Alzheimer’s disease
    Article Snippet: .. In human brain tissue, the goat antiserum sc-1784 and the mouse monoclonal antibody MAB2791 (R&D Systems) demonstrated distinct neuronal CCL2 labeling comparable to the staining pattern observed in mouse brain. ..

    Multiplex Assay:

    Article Title: Transcriptomic analysis of the human habenula in schizophrenia
    Article Snippet: .. smFISH experiments were performed on postmortem human brain tissue using RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) and 4-Plex Ancillary Kit as previously described . ..

    other:

    Article Title: FIN-Seq: Transcriptional profiling of specific cell types in frozen archived tissue from the human central nervous system
    Article Snippet: FFPE adult human cerebral cortex tissue from a 54-year-old female was obtained from Abcam (ab4296).



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    Image Search Results


    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Inhibition of cell surface GRP78 on brain tumors reverses drug resistance and stops cancer stem cell expansion

    doi: 10.1016/j.jbc.2026.111146

    Figure Lengend Snippet: Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Article Snippet: Briefly, three human brain tumors and normal brain tissue microarrays (T174c; Biomax. us) were deparaffinized with xylene and washed with PBS.

    Techniques: Staining, Microarray