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normal osteoblast cell line  (ATCC)


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    Structured Review

    ATCC normal osteoblast cell line
    Normal Osteoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+osteoblasts/hFOB+1%2E19/pm41952154-127-12-37
    Average 98 stars, based on 840 article reviews
    normal osteoblast cell line - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..

    Article Title: Nitrogen-doped carbon nanodots as a biocompatible FRET donor for caspase-3 detection and chemotherapy response evaluation in osteosarcoma cell lysates.
    Article Snippet: 1 Department of Orthopedics, Affiliated Hospital of Yangzhou University, Yangzhou University, No. 368 Hanjiang Middle Road, Hanjiang District, Yangzhou City, Jiangsu Province, China Abstract Osteosarcoma is a highly aggressive bone malignancy requiring precise tools for early diagnosis and effective evaluation of chemotherapy efficacy.. Here, we report an “off–on” fluorescent nanosensor based on nitrogen-doped carbon nanodots (N-CNDs) for the sensitive and selective detection of caspase-3 activity, a key apoptosis executor.. The sensor is constructed by conjugating carboxyl-functionalized N-CNDs with a BHQ1-labeled peptide substrate containing the caspase3-specific DEVD cleavage motif.

    Article Title: Porphyromonas gingivalis lipopolysaccharide induces osteoblast senescence and defective mineralization associated with indoleamine 2,3-dioxygenase 1-kynurenine-aryl hydrocarbon receptor signaling.
    Article Snippet: Aim: To determine whether Porphyromonas gingivalis-derived lipopolysaccharide (P.g-LPS) is associated with osteoblast senescence and defective mineralization involving inflammation-related indoleamine 2,3-dioxygenase 1 (IDO1)–kynurenine (Kyn)–aryl hydrocarbon receptor (AhR) pathway and to examine its upstream regulation.. Methods: hFOB1.19 human osteoblasts and zebrafish were exposed to P.g-LPS.. Osteogenic differentiation, senescence, IDO1/Kyn/AhR signaling, Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB)/RELA regulation, and rescue by the IDO1 inhibitor epacadostat or AhR inhibitor CH223191 were assessed using staining, qPCR, western blotting, ELISA, gene silencing, and dual-luciferase reporter assays.

    Concentration Assay:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Incubator:

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..

    In Vitro:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..

    Activity Assay:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..

    Functional Assay:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..



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    ATCC normal human fetal osteoblast cell line
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Normal Human Fetal Osteoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC normal osteoblasts
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Normal Osteoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Journal: Biomedicines

    Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

    doi: 10.3390/biomedicines13123039

    Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

    Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence