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normal human fetal osteoblasts  (ATCC)


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    ATCC normal human fetal osteoblasts
    Normal Human Fetal Osteoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+osteoblasts/Hfob+1%2E19%3B+Osteoblast%3B+Human/pm41598115-53-0-6
    Average 96 stars, based on 352 article reviews
    normal human fetal osteoblasts - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Crosslinked Gelatin‐Polycaprolactone Scaffolds: Balancing Biocompatibility and Inflammatory Response for Tissue Engineering Applications
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19 ATCC CRL11372) was cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F12, supplemented with 10% FBS and an antibiotic solution containing 1000 IU/mL of penicillin, 100 μg/ mL of streptomycin, and 0.3 μg/mL of fungizone. ..

    Article Title: ZnO‐Hydroxyapatite‐Coated Ti‐6Al‐4V With Curcumin and Ginger Extract for Load‐Bearing Implants
    Article Snippet: .. Human fetal osteoblast cells (hFOB 1.19, obtained from ATCC, Manassas, VA, USA) were cultured on the fabricated samples for 3, 7, and 11 days [ 33 ]. .. The cell culture media was made using a 1:1 mixture of Dulbecco’s modified Eagle’s medium and Ham’s F12 medium (DMEM/F12, Sigma, St. Louis, MO, USA).

    Modification:

    Article Title: Crosslinked Gelatin‐Polycaprolactone Scaffolds: Balancing Biocompatibility and Inflammatory Response for Tissue Engineering Applications
    Article Snippet: .. The human fetal osteoblast cell line (hFOB 1.19 ATCC CRL11372) was cultured in Dulbecco's Modified Eagle Medium: Nutrient Mixture F12, supplemented with 10% FBS and an antibiotic solution containing 1000 IU/mL of penicillin, 100 μg/ mL of streptomycin, and 0.3 μg/mL of fungizone. ..

    other:

    Article Title: Multifunctional POSS-based nanoparticles functionalized with silver, SPIONs, and rhamnolipid for antibacterial applications.
    Article Snippet: Nano-engineered materials, particularly those featuring bio-based surface modifications, are emerging as effective tools in combating bacterial infections.. In this study, polyhedral oligomeric silsesquioxane (POSS) nanoparticles were functionalized with silver nanoparticles (Ag), superparamagnetic iron oxide nanoparticles (SPIONs), and the biosurfactant rhamnolipid (RL)—either individually or in combination—to evaluate their antibacterial and antibiofilm activities against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa).. The modified nanoparticles exhibited sizes ranging from 127 to 227 nm and demonstrated superparamagnetic behavior, offering potential for magnetic targeting.

    Suspension:

    Article Title: Crosslinked Gelatin‐Polycaprolactone Scaffolds: Balancing Biocompatibility and Inflammatory Response for Tissue Engineering Applications
    Article Snippet: .. To evaluate the viability of fetal human osteoblast cells (hFOB 1.19; ATCC CRL- 11372), a suspension containing 5 × 104 cells in 300 μL of culture medium was carefully seeded onto the scaffolds corresponding to each experimental group. ..



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    normal human fetal osteoblast cell line  (ATCC)
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    ATCC normal human fetal osteoblast cell line
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
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    normal osteoblasts  (ATCC)
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    ATCC normal osteoblasts
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
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    Image Search Results


    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Journal: Biomedicines

    Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

    doi: 10.3390/biomedicines13123039

    Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

    Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence