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BioIVT Inc human serum complement preserved
Human Serum Complement Preserved, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+complement-preserved+human+serum/normal+complement+preserved+human+serum/us10864268-903-25-27
Average 90 stars, based on 1 article reviews
human serum complement preserved - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: CD47 antibodies, methods, and uses
Article Snippet: 50,000 Wil2-S cells were plated in opaque white 96-well plates in 25 μl RPMI-1640 supplemented with 10% heat inactivated FBS and 0.1 mM NEAA (all reagents from Invitrogen). .. Upon addition of 25 μl of medium supplemented with or without antibodies cells were incubated for 30 minutes at 37° C. Subsequently, 50 μl of human serum (Bioreclamation, complement preserved) was added and cells were incubated at 37° C. for 2 hours. ..

Article Title: The Human Complement Receptor Type 2 (CR2)/CR1 Fusion Protein TT32, a Novel Targeted Inhibitor of the Classical and Alternative Pathway C3 Convertases, Prevents Arthritis in Active Immunization and Passive Transfer Mouse Models
Article Snippet: These kits were used to assess the effect of TT32, TT30, sCR1 (R&D Systems), CR1 1–10 (Alexion) and anti-CR2 mAbs (clones 171, 629 (Alexion) and 1048 (Green Mountain)) on each pathway, according to manufacturers’ instructions. .. TT32 and other compounds were diluted in normal complement-preserved human serum (Bioreclamation, Westbury, NY) and incubated in presence of AP/CP pathway-specific buffer diluent at 37°C. ..

other:

Article Title: Systemic delivery of a splice-switching oligonucleotide heteroduplex corrects splicing in central nervous system and muscle in spinal muscular atrophy mice
Article Snippet: Serum from BALB/C male mice, unfiltered, and complement preserved, was purchased from BioIVT.

Article Title: Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer.
Article Snippet: Vector genomes (vg) and were then quantified by quantitative PCR using a Lightcycler 480 (Roche Applied Sciences).

Article Title: Heterovalent Click Reactions on DNA Origami.
Article Snippet: Nucleic acid nanoparticles (NANPs) fabricated by using the DNA origami method have broad utility in materials science and bioengineering.. Their site-specific, heterovalent functionalization with secondary molecules such as proteins or fluorophores is a unique feature of this technology that drives its utility.. Currently, however, there are few chemistries that enable fast, efficient covalent functionalization of NANPs with a broad conjugate scope and heterovalency.

Serial Dilution:

Article Title: Discovery of APL-1030, a Novel, High-Affinity Nanofitin Inhibitor of C3-Mediated Complement Activation
Article Snippet: .. Then, a serial dilution of APL-1030 (0.001–10 μM) was added to diluted, complement-preserved human serum (individual healthy donors represented in n = 3, BioIVT, Westbury, NY, USA). ..



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SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
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SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
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Average 90 stars, based on 1 article reviews
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Dunn Labortechnik GmbH pooled complement-preserved normal human serum
SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
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Average 90 stars, based on 1 article reviews
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Asbach Medical Products GmbH pooled complement preserved normal human serum
SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different <t>complement</t> pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% <t>NHS</t> containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.
Pooled Complement Preserved Normal Human Serum, supplied by Asbach Medical Products GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+complement-preserved+human+serum/pooled+complement+preserved+normal+human+serum/10__1097_slash_txd__0000000000000533-82-17-24
Average 90 stars, based on 1 article reviews
pooled complement preserved normal human serum - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

Journal: Blood Advances

Article Title: SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb

doi: 10.1182/bloodadvances.2022009028

Figure Lengend Snippet: SAR443809 specifically inhibits AP without inhibiting CP or LP. SAR443809 and its corresponding IgG isotype control were tested in a series of assays to assess their ability to inhibit the different complement pathways. A dose-dilution series of SAR443809 (●) or IgG control antibody (○) were tested in the commercial Wieslab EIA assays for (A) AP, (C) CP, and (D) LP, in which the inhibition of MAC deposition upon pathway specific activation was assessed per manufacturer’s protocol. (B) Antibodies were also tested in AP hemolysis assay. Rabbit erythrocytes were incubated with 10% NHS containing Mg 2+ -EGTA in the presence of varying concentrations of either SAR443809 (●) or the isotype control (○). The extent of hemolysis was quantified using the absorbance of the released hemoglobin in the supernatant. The data reported here is a mean of 3 independent experiments ± standard deviation, for all 4 data sets.

Article Snippet: Complement preserved normal human serum (NHS) was sourced from Complement Technology Inc (Tyler, TX), BioIVT (Westbury, NY), or Innovative Research (Novi, MI).

Techniques: Control, Inhibition, Activation Assay, Hemolysis Assay, Incubation, Standard Deviation