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normal brain controls  (TaKaRa)


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    TaKaRa normal brain controls
    Normal Brain Controls, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+brain+controls/Human+Brain%2C+Cerebellum+Total+RNA/pm25620587-54-0-14
    Average 94 stars, based on 71 article reviews
    normal brain controls - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37.
    Article Snippet: .. Characterization of DAB1 mRNA variants expression in hNSC The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. .. For PCR reaction, an initial denaturation, at 95◦C for 5 min, was followed by 32 cycles of amplification (94◦C 30 s, 60◦C 30 s and 72◦C 30 s) and a final extension, at 70◦C for 10 min. Immunofluorescence For iPSC lines, pluripotency markers OCT4, SOX2, TRA-1-60 and SSEA4 expression was confirmed with rabbit OCT4, rat SOX2, mouse SSEA4 and mouse TRA-1-60 primary antibodies combined with Alexa Fluor 594 Donkey anti-Rabbit, Alexa Fluor 488 Donkey anti-Rat, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse secondary antibodies, using PSC 4-Marker Immunocytochemistry Kit (#A24881, Thermo Fisher Scientific), following the manufacturer’s instructions.

    Article Title: The Insertion of an ATTTC Repeat in an Alu Element Hyperactivates a Primate-Specific Neurodevelopmental Enhancer in Spinocerebellar Ataxia Type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described ( ). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Polymerase Chain Reaction:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37.
    Article Snippet: .. Characterization of DAB1 mRNA variants expression in hNSC The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. .. For PCR reaction, an initial denaturation, at 95◦C for 5 min, was followed by 32 cycles of amplification (94◦C 30 s, 60◦C 30 s and 72◦C 30 s) and a final extension, at 70◦C for 10 min. Immunofluorescence For iPSC lines, pluripotency markers OCT4, SOX2, TRA-1-60 and SSEA4 expression was confirmed with rabbit OCT4, rat SOX2, mouse SSEA4 and mouse TRA-1-60 primary antibodies combined with Alexa Fluor 594 Donkey anti-Rabbit, Alexa Fluor 488 Donkey anti-Rat, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse secondary antibodies, using PSC 4-Marker Immunocytochemistry Kit (#A24881, Thermo Fisher Scientific), following the manufacturer’s instructions.

    Article Title: The Insertion of an ATTTC Repeat in an Alu Element Hyperactivates a Primate-Specific Neurodevelopmental Enhancer in Spinocerebellar Ataxia Type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described ( ). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Synthesized:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37.
    Article Snippet: .. Characterization of DAB1 mRNA variants expression in hNSC The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. .. For PCR reaction, an initial denaturation, at 95◦C for 5 min, was followed by 32 cycles of amplification (94◦C 30 s, 60◦C 30 s and 72◦C 30 s) and a final extension, at 70◦C for 10 min. Immunofluorescence For iPSC lines, pluripotency markers OCT4, SOX2, TRA-1-60 and SSEA4 expression was confirmed with rabbit OCT4, rat SOX2, mouse SSEA4 and mouse TRA-1-60 primary antibodies combined with Alexa Fluor 594 Donkey anti-Rabbit, Alexa Fluor 488 Donkey anti-Rat, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse secondary antibodies, using PSC 4-Marker Immunocytochemistry Kit (#A24881, Thermo Fisher Scientific), following the manufacturer’s instructions.

    Article Title: The Insertion of an ATTTC Repeat in an Alu Element Hyperactivates a Primate-Specific Neurodevelopmental Enhancer in Spinocerebellar Ataxia Type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described ( ). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Quantitative RT-PCR:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37.
    Article Snippet: .. Characterization of DAB1 mRNA variants expression in hNSC The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. .. For PCR reaction, an initial denaturation, at 95◦C for 5 min, was followed by 32 cycles of amplification (94◦C 30 s, 60◦C 30 s and 72◦C 30 s) and a final extension, at 70◦C for 10 min. Immunofluorescence For iPSC lines, pluripotency markers OCT4, SOX2, TRA-1-60 and SSEA4 expression was confirmed with rabbit OCT4, rat SOX2, mouse SSEA4 and mouse TRA-1-60 primary antibodies combined with Alexa Fluor 594 Donkey anti-Rabbit, Alexa Fluor 488 Donkey anti-Rat, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse secondary antibodies, using PSC 4-Marker Immunocytochemistry Kit (#A24881, Thermo Fisher Scientific), following the manufacturer’s instructions.

    Article Title: The Insertion of an ATTTC Repeat in an Alu Element Hyperactivates a Primate-Specific Neurodevelopmental Enhancer in Spinocerebellar Ataxia Type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described ( ). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Amplification:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37.
    Article Snippet: .. Characterization of DAB1 mRNA variants expression in hNSC The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. .. For PCR reaction, an initial denaturation, at 95◦C for 5 min, was followed by 32 cycles of amplification (94◦C 30 s, 60◦C 30 s and 72◦C 30 s) and a final extension, at 70◦C for 10 min. Immunofluorescence For iPSC lines, pluripotency markers OCT4, SOX2, TRA-1-60 and SSEA4 expression was confirmed with rabbit OCT4, rat SOX2, mouse SSEA4 and mouse TRA-1-60 primary antibodies combined with Alexa Fluor 594 Donkey anti-Rabbit, Alexa Fluor 488 Donkey anti-Rat, Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse secondary antibodies, using PSC 4-Marker Immunocytochemistry Kit (#A24881, Thermo Fisher Scientific), following the manufacturer’s instructions.

    Article Title: The Insertion of an ATTTC Repeat in an Alu Element Hyperactivates a Primate-Specific Neurodevelopmental Enhancer in Spinocerebellar Ataxia Type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described (Table S2). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: .. The expression of total DAB1 mRNA and DAB1 mRNA variants in hNSC was confirmed by semiquantitative PCR using the primers described ( ). cDNA was synthesized from 1 μg total RNA of hNSC or human cerebellum (#636535, Clontech), using SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (#11752050, Invitrogen, Thermo Fisher Scientific), following the manufacturer’s procedure. cDNA was amplified by standard PCR using 6.25 μM of each primer, 1x Supreme NZYTaq II Green Master Mix (#MB36001, NZYtech), and water up to 50 μL. ..

    Isolation:

    Article Title: Gastrodin ameliorates postoperative cognitive dysfunction in aged mice by promoting nuclear translocation of Nrf2 through activation of AMPK.
    Article Snippet: Background: Perioperative neurocognitive disorder (PND) remains a prevalent complication following anesthesia and surgery.. Recent studies have revealed the therapeutic potential of gastrodin (GAS) in treating cognitive disturbances.. This study delves deeper into the mechanisms through which GAS impacts PND.

    Reverse Transcription:

    Article Title: Gastrodin ameliorates postoperative cognitive dysfunction in aged mice by promoting nuclear translocation of Nrf2 through activation of AMPK.
    Article Snippet: Background: Perioperative neurocognitive disorder (PND) remains a prevalent complication following anesthesia and surgery.. Recent studies have revealed the therapeutic potential of gastrodin (GAS) in treating cognitive disturbances.. This study delves deeper into the mechanisms through which GAS impacts PND.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Gastrodin ameliorates postoperative cognitive dysfunction in aged mice by promoting nuclear translocation of Nrf2 through activation of AMPK.
    Article Snippet: Background: Perioperative neurocognitive disorder (PND) remains a prevalent complication following anesthesia and surgery.. Recent studies have revealed the therapeutic potential of gastrodin (GAS) in treating cognitive disturbances.. This study delves deeper into the mechanisms through which GAS impacts PND.

    Real-time Polymerase Chain Reaction:

    Article Title: Gastrodin ameliorates postoperative cognitive dysfunction in aged mice by promoting nuclear translocation of Nrf2 through activation of AMPK.
    Article Snippet: Background: Perioperative neurocognitive disorder (PND) remains a prevalent complication following anesthesia and surgery.. Recent studies have revealed the therapeutic potential of gastrodin (GAS) in treating cognitive disturbances.. This study delves deeper into the mechanisms through which GAS impacts PND.



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