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Johns Hopkins HealthCare nonlin program
Nonlin Program, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonlin/nonlin+program/pm09220970-156-15-9
Average 90 stars, based on 1 article reviews
nonlin program - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Measurement of spontaneous transfer and transbilayer movement of BODIPY-labeled lipids in lipid vesicles.
Article Snippet: An assay was developed to study the spontaneous transfer and transbilayer movement (flipflop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC).. The assay is based on the concentration-dependent changes in fluorescence intensity that occur when donor vesicles containing a C5-DMB-lipid are mixed with nonfluorescent acceptor vesicles.. A kinetic model was developed to describe the time-dependent changes in concentration of a lipid undergoing both spontaneous transfer between unilamellar vesicles and transbilayer movement within the vesicle membranes, and a mathematical solution was obtained.



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