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non-contact inkjet microarray  (Arrayjet Limited)

 
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    Arrayjet Limited non-contact inkjet microarray
    (A) An example slide in which immunoglobulins in sera from different children were assayed. Each <t>microarray</t> slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.
    Non Contact Inkjet Microarray, supplied by Arrayjet Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-contact+inkjet+microarray/arrayjet+microarrayer/bio_rxiv__462689-44-9-13
    Average 90 stars, based on 1 article reviews
    non-contact inkjet microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Comprehensive profiling of antibodies against multiple infectious diseases in serum and the airway mucosa using synthetic peptide-based linear epitope microarrays"

    Article Title: Comprehensive profiling of antibodies against multiple infectious diseases in serum and the airway mucosa using synthetic peptide-based linear epitope microarrays

    Journal: bioRxiv

    doi: 10.1101/462689

    (A) An example slide in which immunoglobulins in sera from different children were assayed. Each microarray slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.
    Figure Legend Snippet: (A) An example slide in which immunoglobulins in sera from different children were assayed. Each microarray slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.

    Techniques Used: Microarray, Incubation, Fluorescence, Selection, Positive Control, Negative Control

    The kinetics of maternal antibody decay were evaluated for five antigens on the peptide microarray chip. Changes in the levels of antigen-specific IgG in (A) serum and (B) airway mucosa, were analysed using loess curve fitting. For all antigens except SPNE, there was evidence of serum IgG decline in the first three months of life. In the airway mucosa similar changes in antigen specific IgG were observed. The dashed red lines indicate the 6-month age time point while the blue dashed line indicates the 12-month time point.
    Figure Legend Snippet: The kinetics of maternal antibody decay were evaluated for five antigens on the peptide microarray chip. Changes in the levels of antigen-specific IgG in (A) serum and (B) airway mucosa, were analysed using loess curve fitting. For all antigens except SPNE, there was evidence of serum IgG decline in the first three months of life. In the airway mucosa similar changes in antigen specific IgG were observed. The dashed red lines indicate the 6-month age time point while the blue dashed line indicates the 12-month time point.

    Techniques Used: Peptide Microarray

    Related Articles

    Modification:

    Article Title: Novel neutralizing chicken IgY antibodies targeting 17 potent conserved peptides identified by SARS-CoV-2 proteome microarray, and future prospects.
    Article Snippet: All SARS-CoV-2 N-, and S-proteins were obtained from Sino Biological, Inc. .. The peptides and proteins were printed onto a 3D modified slide surface in duplicate using an Arrayjet microarrayer (20). ..

    Article Title: Efficacy and safety of immunotherapy combined with single-agent chemotherapy as second- or later-line therapy for metastatic non-small cell lung cancer
    Article Snippet: .. Antibodies were diluted to 200 μg/ml with 5% glycerol and then printed onto a 3D modified slide surface (Capital Biochip Corp, Beijing, China) using an Arrayjet microarrayer (Roslin, UK). ..

    Article Title: Profiling of SARS‐CoV‐2 neutralizing antibody‐associated antigenic peptides signature using proteome microarray
    Article Snippet: SARS‐CoV‐2 proteins, including S (Val16–Pro1213), S1 (Val16‐Arg685), S2 (Ser686‐Pro1213), RBD (Arg319‐Phe541), and N (Met1‐Ala419), were expressed in insect cells or human HEK293 cells (Sino Biological). .. These peptides and proteins were printed onto a three‐dimensional modified slide surface (Capital Biochip Corp) in parallel and duplicated using an Arrayjet microarrayer (Arrayjet). .. PBS, BSA (100 μg/mL; Sigma–Aldrich), and HA peptides (500 μg/mL; Chinese Peptides) were used as negative controls.

    Article Title: Reduced IgG2 with thrombocytopenia predicts mortality in patients with influenza pneumonia.
    Article Snippet: Background: Thrombocytopenia is a common disorder during influenza that is related to high mortality.. Objectives: A prospective study was performed to investigate the association of immunoglobulin subclass changes accompanying incident thrombocytopenia with clinical outcomes in patients with severe influenza.. Methods: 96 influenza patients were recruited and divided into two groups, patients with thrombocytopenia (n = 30) and patients without thrombocytopenia (n = 66).

    Article Title: Novel neutralizing chicken IgY antibodies targeting 17 potent conserved peptides identified by SARS-CoV-2 proteome microarray, and future prospects
    Article Snippet: All SARS-CoV-2 N-, and S-proteins were obtained from Sino Biological, Inc. .. The peptides and proteins were printed onto a 3D modified slide surface in duplicate using an Arrayjet microarrayer ( ). ..

    Negative Control:

    Article Title: Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B
    Article Snippet: .. To measure cytokine levels, anti-human IFNγ capture antibody (Thermo Fisher, Waltham, MA), rabbit anti-SEA antibody (negative control; Toxin Technology), and Alexa647-labeled streptavidin (positive control; Invitrogen) were arrayed onto PATH Protein Microarray slides (Gentel Bioscience, Madison, WI) using an inkjet microarray spotter (ArrayJet, Roslin, Scotland, UK). ..

    Positive Control:

    Article Title: Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B
    Article Snippet: .. To measure cytokine levels, anti-human IFNγ capture antibody (Thermo Fisher, Waltham, MA), rabbit anti-SEA antibody (negative control; Toxin Technology), and Alexa647-labeled streptavidin (positive control; Invitrogen) were arrayed onto PATH Protein Microarray slides (Gentel Bioscience, Madison, WI) using an inkjet microarray spotter (ArrayJet, Roslin, Scotland, UK). ..

    Microarray:

    Article Title: Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B
    Article Snippet: .. To measure cytokine levels, anti-human IFNγ capture antibody (Thermo Fisher, Waltham, MA), rabbit anti-SEA antibody (negative control; Toxin Technology), and Alexa647-labeled streptavidin (positive control; Invitrogen) were arrayed onto PATH Protein Microarray slides (Gentel Bioscience, Madison, WI) using an inkjet microarray spotter (ArrayJet, Roslin, Scotland, UK). ..

    Article Title: Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism.
    Article Snippet: Myoblast proliferation and differentiation are highly dynamic and regulated processes in skeletal muscle development.. Given that proteins serve as the executors for the majority of biological processes, exploring key regulatory factors and mechanisms at the protein level offers substantial opportunities for understanding the skeletal muscle development.. In this study, a total of 607 differentially expressed proteins between proliferation and differentiation in myoblasts were screened out using our chicken muscle antibody array.

    Microscopy:

    Article Title: Antibody array-based proteome approach reveals proteins involved in grape seed development.
    Article Snippet: .. Each antibody was added (10 nL spot−1) to the surface of nitrocellulose-coated microscope slides (FAST chip, CapitalBio, Beijing, China) using an Inkjet Microarrayer (Arrayjet Ltd., Roslin, UK) at 4 °C. ..

    Article Title: Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism.
    Article Snippet: Myoblast proliferation and differentiation are highly dynamic and regulated processes in skeletal muscle development.. Given that proteins serve as the executors for the majority of biological processes, exploring key regulatory factors and mechanisms at the protein level offers substantial opportunities for understanding the skeletal muscle development.. In this study, a total of 607 differentially expressed proteins between proliferation and differentiation in myoblasts were screened out using our chicken muscle antibody array.

    Clinical Proteomics:

    Article Title: Reduced IgG2 with thrombocytopenia predicts mortality in patients with influenza pneumonia.
    Article Snippet: Background: Thrombocytopenia is a common disorder during influenza that is related to high mortality.. Objectives: A prospective study was performed to investigate the association of immunoglobulin subclass changes accompanying incident thrombocytopenia with clinical outcomes in patients with severe influenza.. Methods: 96 influenza patients were recruited and divided into two groups, patients with thrombocytopenia (n = 30) and patients without thrombocytopenia (n = 66).

    Antibody Purification:

    Article Title: Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism.
    Article Snippet: Myoblast proliferation and differentiation are highly dynamic and regulated processes in skeletal muscle development.. Given that proteins serve as the executors for the majority of biological processes, exploring key regulatory factors and mechanisms at the protein level offers substantial opportunities for understanding the skeletal muscle development.. In this study, a total of 607 differentially expressed proteins between proliferation and differentiation in myoblasts were screened out using our chicken muscle antibody array.



    Similar Products

    non-contact inkjet microarray  (Arrayjet Limited)
    90
    Arrayjet Limited non-contact inkjet microarray
    (A) An example slide in which immunoglobulins in sera from different children were assayed. Each <t>microarray</t> slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.
    Non Contact Inkjet Microarray, supplied by Arrayjet Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-contact+inkjet+microarray/arrayjet+microarrayer/bio_rxiv__462689-44-9-13
    Average 90 stars, based on 1 article reviews
    non-contact inkjet microarray - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) An example slide in which immunoglobulins in sera from different children were assayed. Each microarray slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.

    Journal: bioRxiv

    Article Title: Comprehensive profiling of antibodies against multiple infectious diseases in serum and the airway mucosa using synthetic peptide-based linear epitope microarrays

    doi: 10.1101/462689

    Figure Lengend Snippet: (A) An example slide in which immunoglobulins in sera from different children were assayed. Each microarray slide comprised 24 mini-arrays (shown using the white boxes). Two example mini-arrays are enlarged: in the first mini-array a sample obtained from a one-day old neonate was analysed while in the second, serum from a two-month old infant was analysed. On each mini-array, peptide epitopes were printed and serum/mucosal samples incubated. Antibodies in patient samples that bound to peptide epitopes were identified using a cocktail of two detection antibodies: anti human IgG conjugated to Alex Fluor (AF) 647 (red fluorescent signal) and anti-human IgA conjugated to AF-555(green fluorescence). Spots with a yellow hue indicate antigens which were bound by both IgA and IgG in patient samples. Each peptide epitope was printed in duplicate: the locations of a selection of duplicate antigen pairs are shown. (B) The distribution of fluorescence signals from control spots. Each mini-array contained a set of positive control spots (comprising of an anti-human IgG that bound to all IgG immunoglobulins in the patient sample irrespective of antigenic specificity) and negative control spots (microarray printing buffer). Almost all negative control spots did not yield a fluorescent signal - i.e. median fluorescent intensity (MFI)=0. The positive control spots on the other hand yielded much higher MFIs, with most spots resulting in MFIs >10,000.

    Article Snippet: Peptides were printed onto the epoxy slides using a non-contact inkjet microarray printer (Arrayjet, Scotland).

    Techniques: Microarray, Incubation, Fluorescence, Selection, Positive Control, Negative Control

    The kinetics of maternal antibody decay were evaluated for five antigens on the peptide microarray chip. Changes in the levels of antigen-specific IgG in (A) serum and (B) airway mucosa, were analysed using loess curve fitting. For all antigens except SPNE, there was evidence of serum IgG decline in the first three months of life. In the airway mucosa similar changes in antigen specific IgG were observed. The dashed red lines indicate the 6-month age time point while the blue dashed line indicates the 12-month time point.

    Journal: bioRxiv

    Article Title: Comprehensive profiling of antibodies against multiple infectious diseases in serum and the airway mucosa using synthetic peptide-based linear epitope microarrays

    doi: 10.1101/462689

    Figure Lengend Snippet: The kinetics of maternal antibody decay were evaluated for five antigens on the peptide microarray chip. Changes in the levels of antigen-specific IgG in (A) serum and (B) airway mucosa, were analysed using loess curve fitting. For all antigens except SPNE, there was evidence of serum IgG decline in the first three months of life. In the airway mucosa similar changes in antigen specific IgG were observed. The dashed red lines indicate the 6-month age time point while the blue dashed line indicates the 12-month time point.

    Article Snippet: Peptides were printed onto the epoxy slides using a non-contact inkjet microarray printer (Arrayjet, Scotland).

    Techniques: Peptide Microarray