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cellular debris  (OriGene)


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    OriGene cellular debris
    Cellular Debris, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Recombinant+Protein/pm29475084-144-2-22
    Average 90 stars, based on 2 article reviews
    cellular debris - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Full-length myocilin protein is purified from mammalian cells as a dimer.
    Article Snippet: Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH) and mutations in the MYOC gene are the most common mutation observed in glaucoma patients.. Human AH analyzed under non-reducing conditions suggests that MYOC is not normally found in a monomeric form, but rather is predominantly dimeric.. Although MYOC was first reported almost 20 years ago, a technical challenge still faced by researchers is an inability to isolate full-length MYOC protein for experimental purposes.

    Isolation:

    Article Title: Full-length myocilin protein is purified from mammalian cells as a dimer.
    Article Snippet: Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH) and mutations in the MYOC gene are the most common mutation observed in glaucoma patients.. Human AH analyzed under non-reducing conditions suggests that MYOC is not normally found in a monomeric form, but rather is predominantly dimeric.. Although MYOC was first reported almost 20 years ago, a technical challenge still faced by researchers is an inability to isolate full-length MYOC protein for experimental purposes.



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    ( A ) Western blots for human <t>MYOC</t> protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells <t>were</t> <t>transiently-transfected</t> with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.
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    ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Journal: PLoS ONE

    Article Title: Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle

    doi: 10.1371/journal.pone.0206801

    Figure Lengend Snippet: ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Article Snippet: NTM5 cells at 70 to 80% confluence were transiently-transfected with FLAG-tagged plasmids (8μg total cDNA per 10cm plate), human MYOC (Origene, RC206556; Accession number NM_000261), or mouse Myoc (Origene, MR224777; Accession NM_010865) using FuGENE6 transfection reagent (Promega, E2691).

    Techniques: Western Blot, Transgenic Assay, SDS Page, Transfection, Plasmid Preparation, Glycoproteomics, Muscles