Review



additional myoc plasmids  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    OriGene additional myoc plasmids
    Additional Myoc Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Tagged+ORF+Clone/pm29475084-100-0-7
    Average 90 stars, based on 5 article reviews
    additional myoc plasmids - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
    Article Snippet: Cell media was DMEM (Gibco, 11995–065) supplemented with 10% FBS (Gibco, 10082147) and 1% P/S (Gibco, 15140–122). .. NTM5 cells at 70 to 80% confluence were transiently-transfected with FLAG-tagged plasmids (8μg total cDNA per 10cm plate), human MYOC (Origene, RC206556; Accession number NM_000261), or mouse Myoc (Origene, MR224777; Accession NM_010865) using FuGENE6 transfection reagent (Promega, E2691). .. All plasmids had been purified using a Qiagen plasmid maxi kit (Qiagen, 12163) and for transfections FuGENE6 was used at a 5:1 ratio with the cDNA.



    Similar Products

    additional myoc plasmids  (OriGene)
    90
    OriGene additional myoc plasmids
    Additional Myoc Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Tagged+ORF+Clone/pm29475084-100-0-7
    Average 90 stars, based on 1 article reviews
    additional myoc plasmids - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    cellular debris  (OriGene)
    90
    OriGene cellular debris
    Cellular Debris, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Recombinant+Protein/pm29475084-144-2-22
    Average 90 stars, based on 1 article reviews
    cellular debris - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    myoc flag cdna  (OriGene)
    90
    OriGene myoc flag cdna
    Myoc Flag Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Tagged+ORF+Clone/pm29475084-137-6-8
    Average 90 stars, based on 1 article reviews
    myoc flag cdna - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    nm 000261  (OriGene)
    90
    OriGene nm 000261
    Nm 000261, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Mass+Spec+Standard/pmc06311499-569-11-15
    Average 90 stars, based on 1 article reviews
    nm 000261 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    human myoc  (OriGene)
    90
    OriGene human myoc
    ( A ) Western blots for human <t>MYOC</t> protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells <t>were</t> <t>transiently-transfected</t> with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.
    Human Myoc, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Tagged+ORF+Clone/pmc06218065-215-18-20
    Average 90 stars, based on 1 article reviews
    human myoc - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Journal: PLoS ONE

    Article Title: Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle

    doi: 10.1371/journal.pone.0206801

    Figure Lengend Snippet: ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Article Snippet: NTM5 cells at 70 to 80% confluence were transiently-transfected with FLAG-tagged plasmids (8μg total cDNA per 10cm plate), human MYOC (Origene, RC206556; Accession number NM_000261), or mouse Myoc (Origene, MR224777; Accession NM_010865) using FuGENE6 transfection reagent (Promega, E2691).

    Techniques: Western Blot, Transgenic Assay, SDS Page, Transfection, Plasmid Preparation, Glycoproteomics, Muscles