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nitrocefin (1 mg/ml solution freshly diluted with water from a 10 mg/ml dmso stock solution)  (BMG Labtech)


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    BMG Labtech nitrocefin (1 mg/ml solution freshly diluted with water from a 10 mg/ml dmso stock solution)
    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
    Nitrocefin (1 Mg/Ml Solution Freshly Diluted With Water From A 10 Mg/Ml Dmso Stock Solution), supplied by BMG Labtech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocefin+solution/nitrocefin++1+mg+ml+solution+freshly+diluted+with+water+from+a+10+mg+ml+dmso+stock+solution+/pmc11039730-342-8-57
    Average 90 stars, based on 1 article reviews
    nitrocefin (1 mg/ml solution freshly diluted with water from a 10 mg/ml dmso stock solution) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "In situ captured antibacterial action of membrane-incising peptide lamellae"

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae

    Journal: Nature Communications

    doi: 10.1038/s41467-024-47708-4

    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
    Figure Legend Snippet: Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Techniques Used: Staining, Cryo-EM Sample Prep, Membrane, Permeability, Incubation, Positive Control, Standard Deviation, Concentration Assay

    Related Articles

    Incubation:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Concentration Assay:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Staining:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Cryo-EM Sample Prep:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Membrane:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Permeability:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Positive Control:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Standard Deviation:

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae
    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).



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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Image Search Results


    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Journal: Nature Communications

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae

    doi: 10.1038/s41467-024-47708-4

    Figure Lengend Snippet: Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Techniques: Staining, Cryo-EM Sample Prep, Membrane, Permeability, Incubation, Positive Control, Standard Deviation, Concentration Assay