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nitrocefin stock solution  (BMG Labtech)


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    BMG Labtech nitrocefin stock solution
    Nitrocefin Stock Solution, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 99/100, based on 20307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocefin+solution/FLUOstar+Omega/pm36200825-83-13-41
    Average 99 stars, based on 20307 article reviews
    nitrocefin stock solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Interactive Effects of Seminal Plasma and Sonchus oleraceus L. Aqueous Extract on Post-Thaw Sperm Kinematics, Oxidative Status, and In Vitro Fertilization Outcomes in Ram Semen.
    Article Snippet: .. Following a 30 min incubation period at 37 ◦C, the fluorescence signal corresponding to DCF formation was recorded using a FLUO star Omega microplate reader (BMG LABTECH, Ortenberg, Germany). ..

    Article Title: Genomic and functional characterisation of exopolysaccharide biosynthesis in marine bacterium Alteromonas sp. PRIM-28 with emulsifying and anti-inflammatory activities.
    Article Snippet: Bacterial exopolysaccharides (EPS) are structurally heterogeneous biopolymers governed by speciesand strain-specific biosynthetic pathways and with diverse biotechnological applications.. The study investigated EPS biosynthesis in a marine bacterium, Alteromonas sp. PRIM-28 through an integrated genomic and experimental approach.. Whole-genome sequencing and functional annotation were performed to identify the gene clusters and regulatory elements associated with EPS biosynthesis.

    Fluorescence:

    Article Title: Interactive Effects of Seminal Plasma and Sonchus oleraceus L. Aqueous Extract on Post-Thaw Sperm Kinematics, Oxidative Status, and In Vitro Fertilization Outcomes in Ram Semen.
    Article Snippet: .. Following a 30 min incubation period at 37 ◦C, the fluorescence signal corresponding to DCF formation was recorded using a FLUO star Omega microplate reader (BMG LABTECH, Ortenberg, Germany). ..

    Article Title: Effects of Alphitobius diaperinus and Tenebrio molitor Larvae Digestates on Intestinal Epithelial Barrier and Antioxidant-Related Gene Expression in Differentiated Caco-2 Cells: An In Vitro Study.
    Article Snippet: The inserts were incubated at 37 ◦C for 30 min. At the end of the incubation period, an aliquot was collected from the basolateral compartment and transferred to a black, clear-bottom 96-well microplate (Gibco ThermoFisher Scientific, Waltham, MA, USA). .. LY fluorescence was measured using a FLUOstar Omega microplate (BMG Labtech, Ortenberg, Germany) reader at an excitation wavelength of 485 nm and an emission wavelength of 520 nm. ..

    Article Title: A Peculiar Phospholipid-Binding Specificity and Cell Death-Related Properties of Tubic ( Nicotiana tabacum Tubby-like F-Box Protein 8).
    Article Snippet: .. The FLUOstar OPTIMA reader (BMG Labtech, Ortenberg, Germany) equipped with 405 nm excitation and 520 nm emission filters was used to quantify fluorescence intensities. ..

    Construct:

    Article Title: Nutritional Composition, Essential Oil Profiling, and Post-Digestion Phytochemical and Antioxidant Properties of Fennel Pignut ( Bunium ferulaceum ) Seeds.
    Article Snippet: .. In all assays, calibration curves were constructed using the corresponding standard compounds and a microplate reader (FLUOstar Omega, BMG Labtech, Ortenberg, Germany) was used [28]. ..

    Sensitivity:

    Article Title: The chromosomal genome sequence of the sponge, Rhabdastrella globostellata (Carter, 1883) (Tetractinellida: Ancorinidae) and its associated microbial metagenome sequences.
    Article Snippet: .. Libraries were quantified using the AccuClear Ultra High Sensitivity dsDNA Standards Assay Kit (Biotium) and a FLUOstar Omega plate reader (BMG Labtech). ..

    Cell Culture:

    Article Title: Genomic and functional characterisation of exopolysaccharide biosynthesis in marine bacterium Alteromonas sp. PRIM-28 with emulsifying and anti-inflammatory activities.
    Article Snippet: Bacterial exopolysaccharides (EPS) are structurally heterogeneous biopolymers governed by speciesand strain-specific biosynthetic pathways and with diverse biotechnological applications.. The study investigated EPS biosynthesis in a marine bacterium, Alteromonas sp. PRIM-28 through an integrated genomic and experimental approach.. Whole-genome sequencing and functional annotation were performed to identify the gene clusters and regulatory elements associated with EPS biosynthesis.



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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of <t>nitrocefin</t> degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .
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    Image Search Results


    Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Journal: Nature Communications

    Article Title: In situ captured antibacterial action of membrane-incising peptide lamellae

    doi: 10.1038/s41467-024-47708-4

    Figure Lengend Snippet: Bacterial cells were treated with 5 μM 3K in water for 120 min, and negative-stained with uranyl acetate. a NS-TEM image of a single bacterial cell highlighting the supramolecular structures formed within the cell. Scale bar: 200 nm (i–ii) The striped lamellae are observed protruding up to 100−200 nm deep into the cytoplasm from the bacterial cell wall. The inner and outer membranes (IM and OM) are also marked. Disrupted and diffuse OM and IM regions, and leakage of cell content can be clearly observed at positions where the lamellae enter the cell. Scale bar: 50 nm (for more details, see Supplementary Fig. and Fig. ). Measurements were repeated three times and similar results were obtained. b Cryo-EM image of a single bacterial cell with the supramolecular structures formed. Lamellar morphologies are highlighted by arrows. Scale bar: 200 nm. Measurements were repeated three times and similar results were obtained. c Normalized changes in outer membrane permeability induced by 3K. Normalized initial velocities of nitrocefin degradation following a 20 min incubation of bacterial cells with 3K in PBS at 1, 5, 10, 20, and 50 μM concentrations. Lysozyme was used as a positive control. Data points are the average of four biological replicates. Error bars indicate standard deviation. Normalization was done to the 0 μM nitrocefin concentration. For more details, see Supplementary Fig. .

    Article Snippet: Following incubation of the cells with the peptide, nitrocefin (1 mg/mL solution freshly diluted with water from a 10 mg/mL DMSO stock solution) was added to a 50 μg/mL final concentration, and the chromogenic hydrolysis of nitrocefin was monitored by reading absorbance at 490 nm every 15 s for 100 cycles in a CLARIOstar plus plate reader (BMG Labtech).

    Techniques: Staining, Cryo-EM Sample Prep, Membrane, Permeability, Incubation, Positive Control, Standard Deviation, Concentration Assay