deconvolution software (Nikon)
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Deconvolution Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nis+elements+deconvolution+software/NIS-Elements/pm38844629-174-32-34
Average 99 stars, based on 40098 article reviews
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1) Product Images from "Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images."
Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
Journal: Nature methods
doi: 10.1038/s41592-024-02294-7
Figure Legend Snippet: Fig. 1 | Implementation and benchmarking of Deconwolf. a, Schematic Deconwolf workflow. WSI, whole-slide image. b,c, In silico generated microtubule images before (ground truth) (b) and after adding artificial noise to simulate a real image (c). Maximum z-projection is shown. d, MSE after deconvolving the image in c using the default Deconwolf mode with scaled heavy ball15 acceleration (DW_SHB), or Deconwolf based on the classic Richardson–Lucy deconvolution method (DW_RL)2,3. The dashed vertical lines indicate the number of iterations needed to reach the minimum MSE. e, As in c after deconvolution with Deconwolf (DW) using default settings. it, number of iterations. t, deconvolution time measured on an 8-Core AMD Ryzen 7 3700X machine. f, As in c using DeconvolutionLab2 (DL2) with default settings at 115 iterations. g, As in
Techniques Used: In Silico, Generated
Figure Legend Snippet: Fig. 5 | Deconwolf enables robust detection of individual transcripts in low-magnification smFISH images. a, Breast adenocarcinoma tissue section stained with an smFISH probe targeting MKI67 transcripts (white) and imaged on a widefield microscope using a ×20 air objective, after deconvolution with Deconwolf (DW). Maximum z-projection is shown. Blue, DNA. Scale bars, 200 μm in the large left panel; 10 μm in the two small panels on the right. b,c, Zoom-in of the regions (R1 and R2) marked by the white dashed squares in a. Scale bars, 10 μm. d, Left plot: probability density function (PDF) of smFISH dot fluorescence
Techniques Used: Staining, Microscopy, Fluorescence
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