nexterion® slide h microarray slides (SCHOTT)
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Nexterion® Slide H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc10062637-101-0-8
Average 90 stars, based on 1 article reviews
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1) Product Images from "A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time"
Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time
Journal: PLOS ONE
doi: 10.1371/journal.pone.0283537
Figure Legend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Techniques Used: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane
Figure Legend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).
Techniques Used: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining
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