Review




Structured Review

SCHOTT nexterion® slide h microarray slides
Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) <t> microarray, </t> the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Nexterion® Slide H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc10062637-101-0-8
Average 90 stars, based on 1 article reviews
nexterion® slide h microarray slides - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time"

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

Journal: PLOS ONE

doi: 10.1371/journal.pone.0283537

Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag)  microarray,  the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Figure Legend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Techniques Used: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

(A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).
Figure Legend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Techniques Used: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining

Related Articles

Modification:

Article Title: Vaccine and therapeutic compositions comprising antigen-conjugated viral capsids
Article Snippet: Amine-reactive, NHS-ester modified Nexterion® slide H (Schott, Mainz, Germany) microarray slides were used. .. Amine-reactive, NHS-ester modified Nexterion® slide H (Schott, Mainz, Germany) microarray slides were used. ..

Microarray:

Article Title: Vaccine and therapeutic compositions comprising antigen-conjugated viral capsids
Article Snippet: Amine-reactive, NHS-ester modified Nexterion® slide H (Schott, Mainz, Germany) microarray slides were used. .. Amine-reactive, NHS-ester modified Nexterion® slide H (Schott, Mainz, Germany) microarray slides were used. ..

Article Title: A Tympanic Piezo‐Bioreactor Modulates Ion Channel‐Associated Mechanosignaling to Stabilize Phenotype and Promote Tenogenesis in Human Tendon‐Derived Cells.
Article Snippet: .. Nexterion slide H microarray slides were purchased from Schott AG (Mainz, Germany). .. Alexa Fluor 555 carboxylic acid succinimidyl ester was obtained from Life Technologies (Carlsbad, CA, USA).

Article Title: Micromotion Derived Fluid Shear Stress Mediates Peri-Electrode Gliosis through Mechanosensitive Ion Channels.
Article Snippet: .. Protein Antibody Microarray: Nexterion slide H microarray slides were acquired from Schott AG (Mainz, Germany). .. CF 555, succinimidyl ester was purchased from Sigma-Aldrich (SCJ4600022, Dublin, Ireland).

other:

Article Title: Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels
Article Snippet: Sample protein concentrations were measured using Pierce BCA Protein Assay Kit (23227, Thermo Scientific, Waltham, USA) and 4× Laemmli sample buffer containing 200 m m of DTT (11583786001, Sigma‐Aldrich, Dublin, Ireland) was added to 10 μg of protein before being denatured at 95 °C for 5 min. All samples were then separated through a 10% SDS‐PAGE gel at 120 V and then transferred to 0.45 μm pore size nitrocellulose blotting membranes (10600002, Amersham Protran) using a Trans‐Blot Turbo Transfer System (Bio‐Rad, Watford, UK).

Article Title: Enrichment of sweat-derived extracellular vesicles of human and bacterial origin for biomarker identification
Article Snippet: Labeled samples were then cleared by centrifugation (12,000× g for 10 min in RT) and applied onto pre-printed and pre-quenched (with 50 mM ethanolamine) Nexterion H microarray slides (Schott, Germany), and further incubated in a humidified chamber with constant agitation for 2 h in RT.


Article Title: Micromotion derived fluid shear stress mediates peri-electrode gliosis through mechanosensitive ion channels
Article Snippet: CF 555, succinimidyl ester was purchased from Sigma-Aldrich (SCJ4600022, Dublin, Ireland).

Article Title: Lectin microarray profiling demonstrates equivalent global glycosylation for whey protein ingredients enriched with α-lactalbumin and milk fat globule membrane.
Article Snippet: Human milk fat globule membrane (MFGM) and whey proteins are nutritionally and functionally valuable, with many beneficial bioactivities associated with their glycosylation.. However glycosylation of milk components other than free milk oligosaccharides are underinvestigated.. Whey protein concentrate (WPC) ingredients with various enrichments or depletions are used in infant formula (IF) formulations to contribute to human milk equivalence and bioactivity benefits, but their overall or global glycosylation has not been compared.



Similar Products

90
SCHOTT nexterion slide h microarray slides
Nexterion Slide H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc11615817-469-0-8
Average 90 stars, based on 1 article reviews
nexterion slide h microarray slides - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
SCHOTT nexterion h microarray slides
Nexterion H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc10750121-71-17-28
Average 90 stars, based on 1 article reviews
nexterion h microarray slides - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
SCIENION nexterion® h microarray slides
Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) <t> microarray, </t> the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Nexterion® H Microarray Slides, supplied by SCIENION, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc10062637-145-12-36
Average 90 stars, based on 1 article reviews
nexterion® h microarray slides - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
SCHOTT nexterion® slide h microarray slides
Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) <t> microarray, </t> the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Nexterion® Slide H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc10062637-101-0-8
Average 90 stars, based on 1 article reviews
nexterion® slide h microarray slides - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Microarrays Inc nexterion slide h-3d hydrogel coated glass microarray slides
Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) <t> microarray, </t> the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
Nexterion Slide H 3d Hydrogel Coated Glass Microarray Slides, supplied by Microarrays Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nexterion+h+microarray+slides/glass+slides/pmc09931048__SC___014___D2SC06925C___s001-119-22-31
Average 90 stars, based on 1 article reviews
nexterion slide h-3d hydrogel coated glass microarray slides - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag)  microarray,  the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

(A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining

Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag)  microarray,  the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

(A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining