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next-generation sequencing workflow  (Oxford Nanopore)

 
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    Oxford Nanopore next-generation sequencing workflow
    Next Generation Sequencing Workflow, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/next-generation+sequencing+data+analysis+workflow/workflow/pm39491969-36-4-9
    Average 90 stars, based on 1 article reviews
    next-generation sequencing workflow - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Using Vesicular Stomatitis Virus as a Platform for Directed Protease Evolution.
    Article Snippet: .. We therefore established a Nanopore (Oxford Nanopore Technologies) sequencing workflow, which is size-independent and allows up to 96 samples per run and sequencer. .. VSV plates (see Basic Protocol) E.Z.N.A. viral RNA kit (Omega Bio-Tek Inc, cat. no. R6874, or equivalent) NUCLISENS easyMAG high-throughput nucleic acid extraction device RevertAid cDNA synthesis kit (Thermo Fisher Scientific, cat. no. K1622, or equivalent) Q5 polymerase (New England Biolabs, cat. no. M0491S) H2O, sterile, molecular biology grade Rapid barcoding kit (Oxford Nanopore, cat. no. SQK-RBK110.96, or later versions) 1.5-ml microcentrifuge tubes 0.2-ml thin-walled PCR tubes Thermal cycler or heat block Spectrophotometer (e.g., Nanodrop) MinION Mk1B sequencer (Oxford Nanopore) MinKNOW software Geneious Prime (or equivalent clone manager software) Costacurta et al. 11 of 23 Current Protocols nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70074, W iley O nline L ibrary on [25/12/2024].

    Article Title: Using Vesicular Stomatitis Virus as a Platform for Directed Protease Evolution
    Article Snippet: .. We therefore established a Nanopore (Oxford Nanopore Technologies) sequencing workflow, which is size‐independent and allows up to 96 samples per run and sequencer. .. VSV plates (see Basic Protocol) E.Z.N.A. viral RNA kit (Omega Bio‐Tek Inc, cat. no. R6874, or equivalent) NUCLISENS easyMAG high‐throughput nucleic acid extraction device RevertAid cDNA synthesis kit (Thermo Fisher Scientific, cat. no. K1622, or equivalent) Q5 polymerase (New England Biolabs, cat. no. M0491S) H 2 O, sterile, molecular biology grade Rapid barcoding kit (Oxford Nanopore, cat. no. SQK‐RBK110.96, or later versions) 1.5‐ml microcentrifuge tubes 0.2‐ml thin‐walled PCR tubes Thermal cycler or heat block Spectrophotometer (e.g., Nanodrop) MinION Mk1B sequencer (Oxford Nanopore) MinKNOW software Geneious Prime (or equivalent clone manager software)

    other:

    Article Title: Nanopore sequencing enables novel detection of deuterium incorporation in DNA
    Article Snippet: Top center: Bacterial DNA is sequenced using canonical Oxford nanopore sequencing workflow.

    Article Title: Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses.
    Article Snippet: Here the outlook is very positive, since long read sequencing technologies, such as the Oxford Nanopore Technology (ONT) workflow implemented in our study, will allow the recovery of many more complete viral genomes over the next decade.

    Article Title: Detection of DNA methylation from buccal swabs using nanopore sequencing to study stunting.
    Article Snippet: Adaptive sampling is a nextgeneration sequencing workflow developed by Oxford Nanopore Technology.

    Article Title: Advances in forensic genetics: Exploring the potential of long read sequencing.
    Article Snippet: DNA-based technologies have been used in forensic practice since the mid-1980s.. While PCR-based STR genotyping using Capillary Electrophoresis remains the gold standard for generating DNA profiles in routine casework worldwide, the research community is continually seeking alternative methods capable of providing additional information to enhance discrimination power or contribute with new investigative leads.. Oxford Nanopore Technologies (ONT) and PacBio third-generation sequencing have revolutionized the field, offering real-time capabilities, single-molecule resolution, and long-read sequencing (LRS).

    Article Title: Evaluation of Oxford Nanopore Technologies workflows for genomic epidemiology of outbreak‐associated bacterial isolates in the clinical setting
    Article Snippet: Here, we investigate the potential of a new Oxford Nanopore Technologies (ONT) workflow for WGS of clinically relevant bacterial isolates.

    Sampling:

    Article Title: Detection of DNA methylation from buccal swabs using nanopore sequencing to study stunting
    Article Snippet: .. Adaptive sampling is a next-generation sequencing workflow developed by Oxford Nanopore Technology. ..

    Next-Generation Sequencing:

    Article Title: Detection of DNA methylation from buccal swabs using nanopore sequencing to study stunting
    Article Snippet: .. Adaptive sampling is a next-generation sequencing workflow developed by Oxford Nanopore Technology. ..



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