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next generation sequencing data  (Novogene)

 
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    Novogene next generation sequencing data
    Next Generation Sequencing Data, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/next+generation+sequencing+data/generation+next+sequencing/pmc13190337-440-0-7
    Average 86 stars, based on 1 article reviews
    next generation sequencing data - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: The surviving cells were then cultured with IL-2 to allow 389 expansion for an additional 7 days, after which they were harvested for genomic DNA 390 extraction (Thermo, K0721). .. The sgRNA inserts were PCR-amplified, purified, and 391 subjected to next-generation sequencing analysis (Novogene Co., Ltd.). ..

    Purification:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: The surviving cells were then cultured with IL-2 to allow 389 expansion for an additional 7 days, after which they were harvested for genomic DNA 390 extraction (Thermo, K0721). .. The sgRNA inserts were PCR-amplified, purified, and 391 subjected to next-generation sequencing analysis (Novogene Co., Ltd.). ..

    Next-Generation Sequencing:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: The surviving cells were then cultured with IL-2 to allow 389 expansion for an additional 7 days, after which they were harvested for genomic DNA 390 extraction (Thermo, K0721). .. The sgRNA inserts were PCR-amplified, purified, and 391 subjected to next-generation sequencing analysis (Novogene Co., Ltd.). ..

    Article Title: Development of a novel immune infiltration-based gene signature to predict prognosis and immunotherapy response of a novel anti-PD-L1/TGF-β bifunctional fusion protein in recurrent cervical cancer
    Article Snippet: Total RNA was isolated from PD patients before and after the 1st cycle of treatment, and from PR patients before and after the 1st, 15th, and 30th cycles of treatment, using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. .. Next-generation sequencing was performed by Novogene Co., LTD (Beijing, China). ..

    Article Title: Reduced ULK1 links impaired autophagy and mitophagy to Alzheimer’s disease pathology
    Article Snippet: .. Next-generation sequencing data (performed by the company Novogene) were obtained and analyzed. ..

    Article Title: Establishment of an in vitro pingyangmycin-induced mutagenesis system for litchi via embryogenic callus regeneration
    Article Snippet: .. High-quality DNA was sent to Novogene (Beijing, China) for next-generation sequencing library preparation. .. Paired-end whole-genome sequencing was performed on the Illumina NovaSeq 6000 platform.

    Article Title: The therapeutic potential of phage pT2784 against ST40-KL47 type Acinetobacter baumannii and bacterial fitness trade-offs
    Article Snippet: .. Phage and bacterial genomic DNA were subjected to next-generation sequencing at Novogene Bioinformatics Technology Co., Ltd. ..

    Analysis:

    Article Title: Capicua mediates interleukin-2 withdrawal–induced death of activated CD8+ T cells
    Article Snippet: The surviving cells were then cultured with IL-2 to allow 389 expansion for an additional 7 days, after which they were harvested for genomic DNA 390 extraction (Thermo, K0721). .. The sgRNA inserts were PCR-amplified, purified, and 391 subjected to next-generation sequencing analysis (Novogene Co., Ltd.). ..

    Isolation:

    Article Title: Burst of aneuploidy: a cost of adaptation driven by breakdown of cell cycle control.
    Article Snippet: .. DNA was isolated using Genomic Mini AX Yeast (A&A Biotechnology), and nextgeneration sequencing was performed by Novogene for high coverage sequencing (PE 150, expected read depth ~80X). .. The resulting reads were mapped using BWA-MEM (BurrowsWheeler Aligner for Maximum Exact Matches) (v0.717-r1188) (Li and Durbin, 2009) to a yeast reference genome: S. cerevisiae R64-11 supplemented with the kanMX4 cassette sequence.

    Sequencing:

    Article Title: Burst of aneuploidy: a cost of adaptation driven by breakdown of cell cycle control.
    Article Snippet: .. DNA was isolated using Genomic Mini AX Yeast (A&A Biotechnology), and nextgeneration sequencing was performed by Novogene for high coverage sequencing (PE 150, expected read depth ~80X). .. The resulting reads were mapped using BWA-MEM (BurrowsWheeler Aligner for Maximum Exact Matches) (v0.717-r1188) (Li and Durbin, 2009) to a yeast reference genome: S. cerevisiae R64-11 supplemented with the kanMX4 cassette sequence.

    other:

    Article Title: Mitochondrial genomes of Terebelliformia: gene rearrangements, phylogenetic relationships, and evolutionary insights
    Article Snippet: The samples were subsequently sent to Beijing Novogene Co., Ltd., where the complete mitochondrial genome sequences were obtained using next-generation sequencing (NGS).

    Article Title: Inhibiting glycan degradation prevents HIV-induced inflammaging and cognitive impairment.
    Article Snippet: Total RNA was sent to Novogene Co. for next-generation sequencing (mRNA sequencing).



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    Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

    Journal: Genome Biology

    Article Title: KPop: accurate and scalable comparative analysis of microbial genomes by sequence embeddings

    doi: 10.1186/s13059-025-03585-8

    Figure Lengend Snippet: Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

    Article Snippet: In order to do so, simulated next-generation sequencing (NGS) data was generated for each genome using ART [ ], emulating Illumina HiSeq 2500 paired-end reads of length 150 bp with an average coverage of 20-fold.

    Techniques: Sequencing