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Shanghai Genesky Biotech Co next-generation sequencing ngs
Next Generation Sequencing Ngs, supplied by Shanghai Genesky Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+sequencing+data/next+generation+sequencing/pmc08334725-232-0-22
Average 90 stars, based on 1 article reviews
next-generation sequencing ngs - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Effect of SYTL3 - SLC22A3 Variants, Their Haplotypes, and G × E Interactions on Serum Lipid Levels and the Risk of Coronary Artery Disease and Ischaemic Stroke
Article Snippet: Next-generation sequencing technology (NGS) was used to analyse the genotypes of the 6 selected SNPs at the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co. Ltd., China ( ). depicts the relevant primer sequences.

Article Title: Effects of USF1 SNPs and SNP–Environment Interactions on Serum Lipid Profiles and the Risk of Early-Onset Coronary Artery Disease in the Chinese Population
Article Snippet: The six SNPs were genotyped using next-generation sequencing (NGS) technology at the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co. Ltd., China ( ).

Article Title: SYTL3 – SLC22A3 Single-Nucleotide Polymorphisms and Gene–Gene/Environment Interactions on the Risk of Hyperlipidemia
Article Snippet: Next-generation sequencing technology (NGS) was used to analyze the genotypes of the six selected SNPs at the Center for Human Genetics Research, Shanghai Genesky Bio-Tech Co. Ltd., Shanghai, China ( ). depicts all relevant primer sequences.

Article Title: Compound Heterozygous Variants of the CPAMD8 Gene Co-Segregating in Two Chinese Pedigrees With Pigment Dispersion Syndrome/Pigmentary Glaucoma
Article Snippet: Whole-exome sequencing (WES) was performed on three patients in pedigree 1 and the proband in pedigree 2 by Shanghai Genesky Biotechnologies, Inc., and Shanghai WeHealth BioMedical Technology, Co., Ltd, respectively, according to previously described methods ( ).

Construct:

Article Title: Allergen-specific sublingual immunotherapy altered gut microbiota in patients with allergic rhinitis
Article Snippet: .. Subsequently, the libraries were constructed using the NEBNext ® UltraTM DNA Libraries prep kit for Illumina (NEB, USA), and subjected to 2 × 150 bp paired-end metagenomic shotgun sequencing using the NovaSeq 6000 platform at Shanghai GeneSky Biological Technology Co., Ltd. (China). ..

Article Title: Allergen-specific sublingual immunotherapy altered gut microbiota in patients with allergic rhinitis.
Article Snippet: .. Subsequently, the libraries were constructed using the NEBNext® UltraTM DNA Libraries prep kit for Illumina (NEB, USA), and subjected to 2 × 150 bp paired-end metagenomic shotgun sequencing using the NovaSeq 6000 platform at Shanghai GeneSky Biological Technology Co., Ltd. (China). ..

Shotgun Sequencing:

Article Title: Allergen-specific sublingual immunotherapy altered gut microbiota in patients with allergic rhinitis
Article Snippet: .. Subsequently, the libraries were constructed using the NEBNext ® UltraTM DNA Libraries prep kit for Illumina (NEB, USA), and subjected to 2 × 150 bp paired-end metagenomic shotgun sequencing using the NovaSeq 6000 platform at Shanghai GeneSky Biological Technology Co., Ltd. (China). ..

Article Title: Allergen-specific sublingual immunotherapy altered gut microbiota in patients with allergic rhinitis.
Article Snippet: .. Subsequently, the libraries were constructed using the NEBNext® UltraTM DNA Libraries prep kit for Illumina (NEB, USA), and subjected to 2 × 150 bp paired-end metagenomic shotgun sequencing using the NovaSeq 6000 platform at Shanghai GeneSky Biological Technology Co., Ltd. (China). ..

Next-Generation Sequencing:

Article Title: The polysaccharides from the fruits of Lycium barbarum ameliorate high-fat and high-fructose diet-induced cognitive impairment via regulating blood glucose and mediating gut microbiota.
Article Snippet: High-fat and high-fructose diet (HFFD) consumption can induce cognitive dysfunction and gut microbiota disorder.. In the present study, the effects of the polysaccharides from the fruits of Lycium barbarum L. (LBPs) on HFFD-induced cognitive deficits and gut microbiota dysbiosis were investigated.. The results showed that intervention of LBPs (200 mg/kg/day) for 14 weeks could significantly prevent learning and memory deficits in HFFD-fed mice, evidenced by a reduction of latency and increment of crossing parameters of platform quadrant in Morris water maze test.

Sequencing:

Article Title: Gut microbiome impact on childhood allergic rhinitis and house dust mite IgE responses.
Article Snippet: BACKGROUND: The correlation between the gut microbiota and airway inflammation in childhood allergic rhinitis (AR), particularly concerning allergen exposure, remains insufficiently explored.. This study aimed to link gut microbiota changes with house dust mite (HDM)-specific IgE responses in pediatric AR.. METHODS: Using metagenomic shotgun sequencing, we compared the fecal microbiota of 60 children with HDM-AR to 48 healthy controls (HC), analyzing the link to IgE reactions.

Control:

Article Title: Gut microbiome impact on childhood allergic rhinitis and house dust mite IgE responses.
Article Snippet: BACKGROUND: The correlation between the gut microbiota and airway inflammation in childhood allergic rhinitis (AR), particularly concerning allergen exposure, remains insufficiently explored.. This study aimed to link gut microbiota changes with house dust mite (HDM)-specific IgE responses in pediatric AR.. METHODS: Using metagenomic shotgun sequencing, we compared the fecal microbiota of 60 children with HDM-AR to 48 healthy controls (HC), analyzing the link to IgE reactions.

Software:

Article Title: Gut microbiome impact on childhood allergic rhinitis and house dust mite IgE responses.
Article Snippet: BACKGROUND: The correlation between the gut microbiota and airway inflammation in childhood allergic rhinitis (AR), particularly concerning allergen exposure, remains insufficiently explored.. This study aimed to link gut microbiota changes with house dust mite (HDM)-specific IgE responses in pediatric AR.. METHODS: Using metagenomic shotgun sequencing, we compared the fecal microbiota of 60 children with HDM-AR to 48 healthy controls (HC), analyzing the link to IgE reactions.



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Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

Journal: Genome Biology

Article Title: KPop: accurate and scalable comparative analysis of microbial genomes by sequence embeddings

doi: 10.1186/s13059-025-03585-8

Figure Lengend Snippet: Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

Article Snippet: In order to do so, simulated next-generation sequencing (NGS) data was generated for each genome using ART [ ], emulating Illumina HiSeq 2500 paired-end reads of length 150 bp with an average coverage of 20-fold.

Techniques: Sequencing