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Vertis Biotechnologie whole-genome sequencing
Whole Genome Sequencing, supplied by Vertis Biotechnologie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/next+generation+sequencing+data/next+generation+sequencing/pm24725406-212-3-8
Average 90 stars, based on 1 article reviews
whole-genome sequencing - by Bioz Stars, 2026-09
90/100 stars

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Next-Generation Sequencing:

Article Title: Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea
Article Snippet: .. Ten micrograms of DNA-free RNA was sequenced with next generation sequencing by Vertis Biotechnologie AG. ..

Article Title: A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.
Article Snippet: .. NGS sequencing of RNase A-digested and gel purified RNA fragments from an in vitro transcribed ITS2 fragment (45S rRNA, nt 7143-7500) was performed by Vertis Biotechnologie AG (Weihenstephan, Germany). ..

Article Title: Resource Allocation During the Transition to Diazotrophy in Klebsiella oxytoca
Article Snippet: .. Whole-cell RNA extraction, library preparation and quality control, next-generation sequencing, and read alignment was performed commercially by Vertis Biotechnologie AG (Freising, Germany). .. Cell samples were treated with lysozyme for 15 min in order to extract total RNA, which was then isolated using the mirVana microRNA isolation kit (Ambion).

Article Title: The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.
Article Snippet: .. To test transcriptomic responses to different stresses, exponential phase cultures (OD730 ≈ 0.5) were transferred to three different conditions: (i) high light, 1000 μmol photons/m2/s for 30 min; (ii) high temperature, 45 °C for 30 min; and (iii) darkness, no illumination for 2 h. For transcriptomic sequencing, 30 mL of cultures with or without treatments was collected, and total RNA was extracted, treated by DNase I, and sent to vertis Biotechnologie AG (Freising, Germany) for primary and random cDNA library constructions and next-generation sequencing. ..

other:

Article Title: Unraveling the interplay between a small RNA and RNase E in bacteria
Article Snippet: Whole transcriptome libraries were prepared and sequenced at Vertis Biotechnologie AG (Munich, Germany), applying a standard protocol described previously ( , ).

Article Title: Unraveling the interplay between a small RNA and RNase E in bacteria.
Article Snippet: Whole transcriptome libraries were prepared and sequenced at Vertis Biotechnologie AG (Munich, Germany), applying a standard protocol described previously ( 34 ,35 ).

Article Title: Conditional Hfq Association with Small Noncoding RNAs in Pseudomonas aeruginosa Revealed through Comparative UV Cross-Linking Immunoprecipitation Followed by High-Throughput Sequencing
Article Snippet: For total RNA sequencing, high-throughput sequencing was performed at Vertis Biotechnologie AG, Freising, Germany. cDNA libraries were prepared from total RNA and pooled on an Illumina NextSeq 500 mid-output flow cell and sequenced in single-end mode (1 × 75 cycles).

Article Title: Design, development and application of whole-cell based antibiotic-specific biosensor.
Article Snippet: Synthetic biology techniques hold great promise for optimising the production of natural products by microorganisms.. However, evaluating the phenotype of a modified bacterium represents a major bottleneck to the engineering cycle – particularly for antibiotic-producing actinobacteria strains, which grow slowly and are challenging to genetically manipulate.. Here, 1 These authors equally contributed to this work 2 we report the generation and application of antibiotic-specific whole-cell biosensor derived from TetR transcriptional repressor for use in identifying and optimising antibiotic producers.

Sequencing:

Article Title: A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.
Article Snippet: .. NGS sequencing of RNase A-digested and gel purified RNA fragments from an in vitro transcribed ITS2 fragment (45S rRNA, nt 7143-7500) was performed by Vertis Biotechnologie AG (Weihenstephan, Germany). ..

Article Title: The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.
Article Snippet: .. To test transcriptomic responses to different stresses, exponential phase cultures (OD730 ≈ 0.5) were transferred to three different conditions: (i) high light, 1000 μmol photons/m2/s for 30 min; (ii) high temperature, 45 °C for 30 min; and (iii) darkness, no illumination for 2 h. For transcriptomic sequencing, 30 mL of cultures with or without treatments was collected, and total RNA was extracted, treated by DNase I, and sent to vertis Biotechnologie AG (Freising, Germany) for primary and random cDNA library constructions and next-generation sequencing. ..

Purification:

Article Title: A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.
Article Snippet: .. NGS sequencing of RNase A-digested and gel purified RNA fragments from an in vitro transcribed ITS2 fragment (45S rRNA, nt 7143-7500) was performed by Vertis Biotechnologie AG (Weihenstephan, Germany). ..

In Vitro:

Article Title: A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.
Article Snippet: .. NGS sequencing of RNase A-digested and gel purified RNA fragments from an in vitro transcribed ITS2 fragment (45S rRNA, nt 7143-7500) was performed by Vertis Biotechnologie AG (Weihenstephan, Germany). ..

RNA Extraction:

Article Title: Resource Allocation During the Transition to Diazotrophy in Klebsiella oxytoca
Article Snippet: .. Whole-cell RNA extraction, library preparation and quality control, next-generation sequencing, and read alignment was performed commercially by Vertis Biotechnologie AG (Freising, Germany). .. Cell samples were treated with lysozyme for 15 min in order to extract total RNA, which was then isolated using the mirVana microRNA isolation kit (Ambion).

Control:

Article Title: Resource Allocation During the Transition to Diazotrophy in Klebsiella oxytoca
Article Snippet: .. Whole-cell RNA extraction, library preparation and quality control, next-generation sequencing, and read alignment was performed commercially by Vertis Biotechnologie AG (Freising, Germany). .. Cell samples were treated with lysozyme for 15 min in order to extract total RNA, which was then isolated using the mirVana microRNA isolation kit (Ambion).

cDNA Library Assay:

Article Title: The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973.
Article Snippet: .. To test transcriptomic responses to different stresses, exponential phase cultures (OD730 ≈ 0.5) were transferred to three different conditions: (i) high light, 1000 μmol photons/m2/s for 30 min; (ii) high temperature, 45 °C for 30 min; and (iii) darkness, no illumination for 2 h. For transcriptomic sequencing, 30 mL of cultures with or without treatments was collected, and total RNA was extracted, treated by DNase I, and sent to vertis Biotechnologie AG (Freising, Germany) for primary and random cDNA library constructions and next-generation sequencing. ..



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Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

Journal: Genome Biology

Article Title: KPop: accurate and scalable comparative analysis of microbial genomes by sequence embeddings

doi: 10.1186/s13059-025-03585-8

Figure Lengend Snippet: Data preprocessing workflow. When analyzing NGS datasets with KPop, one can optionally pre-process sequencing reads in order to eliminate biases and/or have the method focus on specific parts of the genome. For instance, one might align reads to a (pan-)genome and separate them into reads that align (likely to originate from the organism being studied) and reads that do not (likely to come from contaminations). Furthermore, reads that do map to the pan-genome might be separated into groups specific to different genomic features; for instance, one might align them to a set of MLST genes or AMR genes. Full k -mer spectra would then be separately obtained from each group of reads (contaminations, pan-genomic, MLST genes, AMR genes) and given as input to downstream/classification methods. The choice of the group of reads from which spectra are computed determines the set of sequences seen by the method, and hence the scope of the classification

Article Snippet: In order to do so, simulated next-generation sequencing (NGS) data was generated for each genome using ART [ ], emulating Illumina HiSeq 2500 paired-end reads of length 150 bp with an average coverage of 20-fold.

Techniques: Sequencing