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anti acsa 2 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti acsa 2 microbeads
    Anti Acsa 2 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuron+isolation+kit/Neuron+Isolation+Kit%2C+mouse+-+small+size/pmc13115098-75-3-5
    Average 96 stars, based on 38 article reviews
    anti acsa 2 microbeads - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Glial-specific mitochondrial failure and redox imbalance contribute to regional vulnerability in Friedreich's ataxia.
    Article Snippet: Specific cell populations were then isolated from this suspension using MACS technology (Miltenyi Biotec) according to the manufacturer's instructions. .. Neurons were isolated using the Neuron Isolation Kit Mouse (Miltenyi Biotec, ref. 130-115-390), and astrocytes were selected using the Anti-ACSA-2 MicroBead Kit, Mouse (Miltenyi Biotec, ref. 130-097-678). ..

    Article Title: Astrocyte Senescence Impairs Synaptogenesis due to Thrombospondin-1 Loss.
    Article Snippet: Embryos were decapitated and hippocampal cell suspension was obtained using the Neural Tissue Dissociation Kit (Miltenyi, 130- 092- 628) in combination with the gentleMACS Octo Dissociator (Miltenyi, 130- 095- 937). .. Neurons were isolated by MACS using the Neuron Isolation Kit (Miltenyi Biotec, 130- 115- 390). ..

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Article Title: RIPK3 acts as a molecular switch between neuroprotection and neuropathology during herpes simplex encephalitis
    Article Snippet: .. Primary cortical neurons were isolated using Neuron cell Isolation kit (Cat130-115-390, Miltenyi) and maintained in Neurobasal media supplied with B-27 (Cat#17504044, Gibco)40,41. ..

    Blocking Assay:

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: .. In total, 10 μL of FcR blocking reagent (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 10 min, Anti-ACSA-2 MicroBeads (Miltenyi #130-115-390) were added and again incubated at 2–8 °C for 15 min.

    Suspension:

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: .. In total, 10 μL of FcR blocking reagent (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 10 min, Anti-ACSA-2 MicroBeads (Miltenyi #130-115-390) were added and again incubated at 2–8 °C for 15 min.

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: .. In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns.

    Incubation:

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: .. In total, 10 μL of FcR blocking reagent (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 10 min, Anti-ACSA-2 MicroBeads (Miltenyi #130-115-390) were added and again incubated at 2–8 °C for 15 min.

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: In total, 10 μL of FcR blocking reagent (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 10 min, Anti-ACSA-2 MicroBeads (Miltenyi #130-115-390) were added and again incubated at 2–8 °C for 15 min. ..

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: .. In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns.

    Magnetic Cell Separation:

    Article Title: Astrocyte Senescence Impairs Synaptogenesis due to Thrombospondin-1 Loss.
    Article Snippet: Embryos were decapitated and hippocampal cell suspension was obtained using the Neural Tissue Dissociation Kit (Miltenyi, 130- 092- 628) in combination with the gentleMACS Octo Dissociator (Miltenyi, 130- 095- 937). .. Neurons were isolated by MACS using the Neuron Isolation Kit (Miltenyi Biotec, 130- 115- 390). ..

    Single Cell:

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Cell Isolation:

    Article Title: RIPK3 acts as a molecular switch between neuroprotection and neuropathology during herpes simplex encephalitis
    Article Snippet: .. Primary cortical neurons were isolated using Neuron cell Isolation kit (Cat130-115-390, Miltenyi) and maintained in Neurobasal media supplied with B-27 (Cat#17504044, Gibco)40,41. ..



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