human neuroblastoma imr 32 cells (ATCC)

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ATCC human neuroblastoma imr 32 cells

Calcium-mediated changes in transcript abundance are enriched for functional gene categories. (a) Hierarchical clustering of the potassium chloride (KCl) transcript dataset identifies six major patterns of gene expression over the 24-hour time course. The number of transcripts contained in each cluster is designated in parentheses. Shown in each plot is the averaged expression behavior of all transcripts in the indicated cluster, with error bars indicating the deviation from the mean for the entire cluster. Expression behavior of each transcript was obtained by subtracting the mean of all points at all times from each expression value in the original time series, and dividing by the standard deviation. KCl-treated time points are indicated in blue. Thapsigargin (TPG) treatment is indicated in red. (b) Gene Ontology (GO) analyses of the transcripts in each cluster reveals functional enrichment in clusters T1 to T4. (c) Polymerase chain reaction performed on cDNA from treated and untreated <t>IMR-32</t> cells validates changes in transcript abundance predicted by the microarray data. PREX1 , a gene whose abundance was not predicted to change, served as a normalization control. (d) Transcripts regulated by KCl stimulation at 24 hours are similarly affected by TPG treatment. Numbers indicate upregulated and downregulated transcripts. Among those transcripts affected by both KCl and TPG, 116 were upregulated and 573 were downregulated. ARRDC3 , arrestin domain containing 3; BNIP3 , BCL2 adenovirus E1B 19 kDa interacting protein 3; ETV5 , ets variant gene 5; GLI3 , GLI-Kruppel family member 3; KCNK7 , potassium channel, subfamily K, member 7; PREX1 , phosphatidylinositol 3,4,5-trisphosphate-dependent RAC exchanger 1; OVGP1 , oviductal glycoprotein 1; STC1 , stanniocalcin 1; ZNF143 , zinc finger protein 143.

Human Neuroblastoma Imr 32 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurobooster+microarray/IMR-32/pmc02374990-255-0-4
Average 97 stars, based on 1290 article reviews

human neuroblastoma imr 32 cells - by Bioz Stars, 2026-11

97/100 stars

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1) Product Images from "Exon expression profiling reveals stimulus-mediated exon use in neural cells"

Article Title: Exon expression profiling reveals stimulus-mediated exon use in neural cells

Journal: Genome Biology

doi: 10.1186/gb-2007-8-8-r159

Figure Legend Snippet: Calcium-mediated changes in transcript abundance are enriched for functional gene categories. (a) Hierarchical clustering of the potassium chloride (KCl) transcript dataset identifies six major patterns of gene expression over the 24-hour time course. The number of transcripts contained in each cluster is designated in parentheses. Shown in each plot is the averaged expression behavior of all transcripts in the indicated cluster, with error bars indicating the deviation from the mean for the entire cluster. Expression behavior of each transcript was obtained by subtracting the mean of all points at all times from each expression value in the original time series, and dividing by the standard deviation. KCl-treated time points are indicated in blue. Thapsigargin (TPG) treatment is indicated in red. (b) Gene Ontology (GO) analyses of the transcripts in each cluster reveals functional enrichment in clusters T1 to T4. (c) Polymerase chain reaction performed on cDNA from treated and untreated IMR-32 cells validates changes in transcript abundance predicted by the microarray data. PREX1 , a gene whose abundance was not predicted to change, served as a normalization control. (d) Transcripts regulated by KCl stimulation at 24 hours are similarly affected by TPG treatment. Numbers indicate upregulated and downregulated transcripts. Among those transcripts affected by both KCl and TPG, 116 were upregulated and 573 were downregulated. ARRDC3 , arrestin domain containing 3; BNIP3 , BCL2 adenovirus E1B 19 kDa interacting protein 3; ETV5 , ets variant gene 5; GLI3 , GLI-Kruppel family member 3; KCNK7 , potassium channel, subfamily K, member 7; PREX1 , phosphatidylinositol 3,4,5-trisphosphate-dependent RAC exchanger 1; OVGP1 , oviductal glycoprotein 1; STC1 , stanniocalcin 1; ZNF143 , zinc finger protein 143.

Techniques Used: Functional Assay, Gene Expression, Expressing, Standard Deviation, Polymerase Chain Reaction, Microarray, Control, Variant Assay

Control:

Article Title: LncRNA GAS6-AS1 enhances the progression of pediatric neuroblastoma by modulating miR-3200-3p.
Article Snippet: .. Human neuroblastoma cells (SK-N-BE(2), SK-N-SH, SK-N-AS, IMR-32, and SH-SY5Y) were obtained from Procell (China), while the human cortical neuronal cell line (HCN-2, control) was acquired from ATCC (USA). .. All cells were maintained in DMEM (Gibco, USA) containing 1% penicillin/streptomycin (Solarbio, China) and 10% serum (Gibco, USA) at 37°C in a 5% CO2 atmosphere.

other:

Article Title: Bistable type II opsins and uses thereof
Article Snippet: Suitable cell lines include, but are not limited to, a human glioma cell line, e.g., SVGp12 (ATCC CRL-8621), CCF-STTG1 (ATCC CRL-1718), SW 1088 (ATCC HTB-12), SW 1783 (ATCC HTB-13), LLN-18 (ATCC CRL-2610), LNZTA3WT4 (ATCC CRL-11543), LNZTA3WT11 (ATCC CRL-11544), U-138 MG (ATCC HTB-16), U-87 MG (ATCC HTB-14), H4 (ATCC HTB-148), and LN-229 (ATCC CRL-2611); a human medulloblastoma-derived cell line, e.g., D342 Med (ATCC HTB-187), Daoy (ATCC HTB-186), D283 Med (ATCC HTB-185); a human tumor-derived neuronal-like cell, e.g., PFSK-1 (ATCC CRL-2060), SK-N-DZ (ATCCCRL-2149), SK-N-AS (ATCC CRL-2137), SK-N-FI (ATCC CRL-2142), IMR-32 (ATCC CCL-127), etc.

Amplification:

Article Title: DNA-PKcs promotes therapy resistance and metastatic recurrence in neuroblastoma
Article Snippet: .. MYCN -amplified human NB cell lines (BE(2)-C (CVCL_V006), IMR-32 (CVCL_0346), LAN-1 (CVCL_1827) and SK-N-DZ (CVCL_1701)) and MYCN -non-amplified cells (SK-N-AS (CVCL_1700), and SK-N-SH (CVCL_0531)) were purchased from ATCC. ..

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