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image labtm software  (Bio-Rad)


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    Bio-Rad image labtm software
    Image Labtm Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nescs/Image+Lab+Software/pm24028773-46-14-17
    Average 99 stars, based on 35737 article reviews
    image labtm software - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad).

    Software:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution. .. Band intensities were quantified using Image Lab software (Bio-Rad). ..

    Article Title: Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann Cells.
    Article Snippet: Membranes were probed with the following antibodies: anti-NF1 #sc-376886 (1:200) by Santa Cruz Biotechnology (Dallas, TX, USA), anti-PARP #10872500 (1:1000) by Roche, anti-NRF2 #PA58804 (1:1000) by Life Technologies, anti-TRX #EPR6110 (1:1000) by Abcam (Cambridge, UK), anti-SOD1 #TA50495 (1:1000) by OriGene Technologies, (Rockville, MD, USA), anti-cleaved caspase-3 (Asp175) #9661 (1:1000) by Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH-HRP #MA5-15738-HRP (1:5000) by Life Technologies. .. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Image Lab software 5.0 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Staining:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Imaging:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Incubation:

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..



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    Establishment of cell polarity in cerebral cortex development. (A) The early neuroepithelium is composed of <t>highly</t> <t>polarized</t> neuroepithelial stem cells <t>(NESCs,</t> apical-basal polarity is indicated). NESCs give rise to radial glia progenitor stem cells (RGPCs) which exhibit even more polarized cellular morphology with an extended basal process. During neurogenesis symmetric radial glia progenitor (RGP) divisions may generate two RGPs but asymmetric divisions produce a renewing RGP and a neuron or an intermediate progenitor (IP). IPs further divide symmetrically in the subventricular zone (SVZ) to produce neurons. The basal processes of RGPs serve as a scaffold for nascent post-mitotic neurons, which migrate in a step-wise fashion coupled with changes in cell polarity, from the ventricular zone (VZ)/SVZ through the intermediate zone (IZ) in order to reach the cortical plate (CP). After nascent cortical projection neurons have delaminated from the neuroepithelium at the ventricular surface they move radially away to the SVZ exhibiting bipolar (BP) morphology. Within the SVZ/IZ, neurons “sojourn” for about 24 h or longer and most adopt a multipolar (MP) morphology, extending and retracting processes in all directions. At one point fundamental cellular polarization events take place that predetermine the future axon of the neuron before the neuron again adopts a bipolar morphology and starts locomoting along the radial glial fiber through the IZ. Once reaching the subplate (SP), neurons enter the CP and migrate towards the marginal zone (MZ) where they detach from the radial glial fiber. Finally, neurons settle in their appropriate position in the CP and the leading process will eventually become the dendrite. (B) This panel depicts the migrating neuron from panel (A) in higher detail with the leading and trailing processes which eventually become the dendrite and axon, respectively.
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    Image Search Results


    Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

    Journal: Human Mutation

    Article Title: Single‐Cell Transcriptomic Profiling and Machine Learning Integration Unveil Stromal Cell Heterogeneity in Endometriosis

    doi: 10.1155/humu/5565366

    Figure Lengend Snippet: Differential expression of key genes in ectopic endometrial cells. qRT‐PCR validation of four key genes (HOXA10, ESR1, MMP9, and SPP1) in normal endometrial stromal cells (NESCs) and ectopic endometrial stromal cells (EESCs). Gene expression was normalized to GAPDH and presented as fold change relative to NESCs. Data are shown as mean ± SD ( n = 3). ∗∗∗ p < 0.001 versus NESCs.

    Article Snippet: NESCs (ATCC Cat# CRL‐4003, RRID:CVCL_D697) and EESCs (ATCC Cat# CRL‐7566, RRID:CVCL_IW41) were authenticated by short tandem repeat (STR) profiling, showing ≥ 95% match to the reference profile in the ATCC database.

    Techniques: Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Gene Expression

    Establishment of cell polarity in cerebral cortex development. (A) The early neuroepithelium is composed of highly polarized neuroepithelial stem cells (NESCs, apical-basal polarity is indicated). NESCs give rise to radial glia progenitor stem cells (RGPCs) which exhibit even more polarized cellular morphology with an extended basal process. During neurogenesis symmetric radial glia progenitor (RGP) divisions may generate two RGPs but asymmetric divisions produce a renewing RGP and a neuron or an intermediate progenitor (IP). IPs further divide symmetrically in the subventricular zone (SVZ) to produce neurons. The basal processes of RGPs serve as a scaffold for nascent post-mitotic neurons, which migrate in a step-wise fashion coupled with changes in cell polarity, from the ventricular zone (VZ)/SVZ through the intermediate zone (IZ) in order to reach the cortical plate (CP). After nascent cortical projection neurons have delaminated from the neuroepithelium at the ventricular surface they move radially away to the SVZ exhibiting bipolar (BP) morphology. Within the SVZ/IZ, neurons “sojourn” for about 24 h or longer and most adopt a multipolar (MP) morphology, extending and retracting processes in all directions. At one point fundamental cellular polarization events take place that predetermine the future axon of the neuron before the neuron again adopts a bipolar morphology and starts locomoting along the radial glial fiber through the IZ. Once reaching the subplate (SP), neurons enter the CP and migrate towards the marginal zone (MZ) where they detach from the radial glial fiber. Finally, neurons settle in their appropriate position in the CP and the leading process will eventually become the dendrite. (B) This panel depicts the migrating neuron from panel (A) in higher detail with the leading and trailing processes which eventually become the dendrite and axon, respectively.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Cell Polarity in Cerebral Cortex Development—Cellular Architecture Shaped by Biochemical Networks

    doi: 10.3389/fncel.2017.00176

    Figure Lengend Snippet: Establishment of cell polarity in cerebral cortex development. (A) The early neuroepithelium is composed of highly polarized neuroepithelial stem cells (NESCs, apical-basal polarity is indicated). NESCs give rise to radial glia progenitor stem cells (RGPCs) which exhibit even more polarized cellular morphology with an extended basal process. During neurogenesis symmetric radial glia progenitor (RGP) divisions may generate two RGPs but asymmetric divisions produce a renewing RGP and a neuron or an intermediate progenitor (IP). IPs further divide symmetrically in the subventricular zone (SVZ) to produce neurons. The basal processes of RGPs serve as a scaffold for nascent post-mitotic neurons, which migrate in a step-wise fashion coupled with changes in cell polarity, from the ventricular zone (VZ)/SVZ through the intermediate zone (IZ) in order to reach the cortical plate (CP). After nascent cortical projection neurons have delaminated from the neuroepithelium at the ventricular surface they move radially away to the SVZ exhibiting bipolar (BP) morphology. Within the SVZ/IZ, neurons “sojourn” for about 24 h or longer and most adopt a multipolar (MP) morphology, extending and retracting processes in all directions. At one point fundamental cellular polarization events take place that predetermine the future axon of the neuron before the neuron again adopts a bipolar morphology and starts locomoting along the radial glial fiber through the IZ. Once reaching the subplate (SP), neurons enter the CP and migrate towards the marginal zone (MZ) where they detach from the radial glial fiber. Finally, neurons settle in their appropriate position in the CP and the leading process will eventually become the dendrite. (B) This panel depicts the migrating neuron from panel (A) in higher detail with the leading and trailing processes which eventually become the dendrite and axon, respectively.

    Article Snippet: NESCs are highly polarized and their nuclei exhibit interkinetic nuclear migration whereby they translocate from the ventricular (apical) side to the more basal side in concert with the cell cycle (Lee and Norden, ).

    Techniques: