nelf-e antibody (Millipore)
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nelf-e antibody
Nelf E Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nelf+e/anti+nelf+e/pm37117180-412-0-2
Average 90 stars, based on 1 article reviews
Nelf E Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nelf+e/anti+nelf+e/pm37117180-412-0-2
Average 90 stars, based on 1 article reviews
nelf-e antibody - by Bioz Stars,
2026-11
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Western Blot:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Transfection:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Injection:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Two Tailed Test:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Expressing:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Wound Healing Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Tube Formation Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Invasion Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Staining:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Incubation:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Flow Cytometry:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Labeling:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Transduction:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Migration:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. shRNA:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Binding Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. ChIP-sequencing:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Immunoprecipitation:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Activation Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Quantitative RT-PCR:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Over Expression:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Plasmid Preparation:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Isolation:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Derivative Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Purification:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Polyacrylamide Gel Electrophoresis:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. SDS Page:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Molecular Weight:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Marker:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Mass Spectrometry:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Generated:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Fluorescence:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Construct:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Mutagenesis:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Produced:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Standard Deviation:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Variant Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Sequencing:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. In Vivo:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. RNA Binding Assay:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Biomarker Discovery:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. Sonication:Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis. Article Snippet: Article Title: Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis Article Snippet: The slides were then blocked in 10% goat serum for 30 min. Article Title: Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes Article Snippet: An amount of 10 μg of Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: 293FT cells were maintained in DMEM (Gibco) with 10% FBS and Glutamax. Article Title: Rapid activity-induced transcription of Arc and other IEGs relies on poised RNA polymerase II. Article Snippet: Sonicated samples were pre-cleared for 1.5 h with 50% A/G bead slurry (Santa Cruz Biotechnology), then immunoprecipitated overnight at 4 °C with 5 μg of 8WG16 antibody (Abcam), antibody to Rpb1 pSer5 CTD (Abcam), antibody to H3 (Abcam), antibody to Article Title: CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing. Article Snippet: Antibodies were diluted in TBS-T (Tris-HCl, pH 8, 150 mM NaCl and Tween 20 0.1%), with 5% dehydrated milk, at the following concentrations: house-made mouse monoclonal anti-PHAX (clones 17E11 and 10E3, 1:5,000 in western blots; validation in Supplementary Fig. 1); rabbit polyclonal anti-ARS2 (Abcam ab88392, 1:1,000 in western blots; validation on manufacturer’s website); rabbit anti-GAPDH (Santa Cruz FL335, 1:1,000 in western blots; validation in ref. 44), Article Title: Architecture and RNA binding of the human negative elongation factor Article Snippet: Cells were routinely checked for mycoplasma contamination using the PlasmoTest Mycoplasma Detection Kit (InvivoGen, 12K06-MM). Article Title: Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia. Article Snippet: Contents lists available at ScienceDirect Neurobiology of Disease j ourna l homepage: www.e lsev ie r .com/ locate /ynbd i Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia Matthieu F. Bastide, Simone Bido, Nathalie Duteil, Erwan Bézard ⁎ a Univ. de Bordeaux, Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France b CNRS,Institut des Maladies Neurodégénératives,UMR 5293,33000 Bordeaux,France ⁎ Corresponding author at: Institut des Maladies Neu Bordeaux,UMR 5293, Bât 3B 1er étage, 146 rue Léo Sai France.. E-mail address: erwan.bezard@u-bordeaux.fr (E. Béza Available online on ScienceDirect (www.sciencedir http://dx.doi.org/10.1016/j.nbd.2015.10.013 0969-9961/© 2015 Elsevier Inc. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 17 September 2015 Revised 7 October 2015 Accepted 14 October 2015 Available online 19 October 2015 Keywords: Immediate-early genes Transcription Stalling Parkinson's disease Rat model Long-term L-3,4-dihydroxyphenylalanine (L-Dopa) treatment in Parkinson's disease leads to involuntary movements called dyskinesia, notably through an overexpression of immediate-early genes (IEG).. Their rapid transcription involves the stalling of RNA polymerase II on IEG promoters, a mechanism that critically depends on the presence of the negative elongation factor (NELF) protein complex. |
