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Sangon Biotech negative control sirna sequence
Negative Control Sirna Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+sequence/control+negative+sirna/pmc12890841-88-17-26
Average 86 stars, based on 1 article reviews
negative control sirna sequence - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Negative Control:

Article Title: miR-2110 orchestrates ERK–ELK1 transcriptional repression to induce cell-cycle arrest and enhance cytarabine sensitivity in acute myeloid leukemia
Article Snippet: .. miR-2110 mimics, negative control (NC) oligonucleotides, and ELK1 overexpression plasmids, along with their corresponding control vectors, were purchased from Sangon Biotech (Shanghai, China). ..

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression
Article Snippet: .. GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). ..

Article Title: Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane
Article Snippet: .. Three small interfering RNA sequences targeting TGF-β1 (si-TGF-β1 #1, si-TGF-β1 #2, si-TGF-β1 #3) and a negative control sequence (si-TGF-β1 NC) were synthesized separately by Sangon Biotech (China). .. The EPCs were seeded into culture plates, and when EPCs confluence reached 60% to 70%, transfection was performed using Lipofectamin 3000 reagent (Thermo Fisher Scientific).

Article Title: Neuronal PRRT3 coordinates amyloidogenic processing and Tau phosphorylation via distinct Ras-ERK-AP-1 and CaMKII/PP2A pathways.
Article Snippet: .. For transfection, PRRT3-specific siRNA (si-PRRT3: 5′-GAGUUUCUCCUGGGAGCUUTT-3′) and negative control siRNA (si-Con: 5′-UUCUCCGAACGUGUCACGUTT-3′), purchased from Sangon Biotech (Shanghai, China), were separately mixed with transfection reagent (CALNPTM RNAi in vitro; DN001-05; D-Nano Therapeutics, Beijing, China) in Opti-MEM (31985070; Gibco) serum-free medium and left at room temperature to form complexes, with reagent amounts added according to the instructions. ..

Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

Article Title: Fisetin alleviates osteoporosis by promoting osteogenesis and suppressing adipogenesis via the Wnt/β-catenin signaling pathway in mice.
Article Snippet: Osteoporosis (OP) is characterized by an imbalance in which bone marrow mesenchymal stem cells (BMSCs) preferentially differentiate into adipocytes rather than osteoblasts.. The natural polyhydroxyflavone fisetin functions as an antioxidant and multi-target signaling regulator with potential to modulate stem cell fate.. However, its role and underlying mechanisms in OP remain unclear.

Over Expression:

Article Title: miR-2110 orchestrates ERK–ELK1 transcriptional repression to induce cell-cycle arrest and enhance cytarabine sensitivity in acute myeloid leukemia
Article Snippet: .. miR-2110 mimics, negative control (NC) oligonucleotides, and ELK1 overexpression plasmids, along with their corresponding control vectors, were purchased from Sangon Biotech (Shanghai, China). ..

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression
Article Snippet: .. GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). ..

Control:

Article Title: miR-2110 orchestrates ERK–ELK1 transcriptional repression to induce cell-cycle arrest and enhance cytarabine sensitivity in acute myeloid leukemia
Article Snippet: .. miR-2110 mimics, negative control (NC) oligonucleotides, and ELK1 overexpression plasmids, along with their corresponding control vectors, were purchased from Sangon Biotech (Shanghai, China). ..

Article Title: Centromere protein I promotes hepatocellular carcinoma progression by activating PI3K/AKT/mTOR-CDK2 cascade
Article Snippet: .. CENPI-targeting siRNAs and a nontargeting control siRNA (si-NC) were synthesized by Sangon Biotech (Shanghai, China). .. HepG2 cells were seeded in 6-well plates at 2 × 10 5 cells/well and transfected at 60%–70% confluence using Lipofectamine 8000 (Beyotime, China, catalog number C0533) in DMEM medium, according to the manufacturer's instructions.

Knockdown:

Article Title: Gamma-aminobutyric acid affects the tumor microenvironment and angiogenesis, promoting breast cancer progression
Article Snippet: .. GAD1 overexpression, GAD1 knockdown, and negative control plasmids were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). ..

Small Interfering RNA:

Article Title: Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane
Article Snippet: .. Three small interfering RNA sequences targeting TGF-β1 (si-TGF-β1 #1, si-TGF-β1 #2, si-TGF-β1 #3) and a negative control sequence (si-TGF-β1 NC) were synthesized separately by Sangon Biotech (China). .. The EPCs were seeded into culture plates, and when EPCs confluence reached 60% to 70%, transfection was performed using Lipofectamin 3000 reagent (Thermo Fisher Scientific).

Article Title: Fisetin alleviates osteoporosis by promoting osteogenesis and suppressing adipogenesis via the Wnt/β-catenin signaling pathway in mice.
Article Snippet: Osteoporosis (OP) is characterized by an imbalance in which bone marrow mesenchymal stem cells (BMSCs) preferentially differentiate into adipocytes rather than osteoblasts.. The natural polyhydroxyflavone fisetin functions as an antioxidant and multi-target signaling regulator with potential to modulate stem cell fate.. However, its role and underlying mechanisms in OP remain unclear.

Sequencing:

Article Title: Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane
Article Snippet: .. Three small interfering RNA sequences targeting TGF-β1 (si-TGF-β1 #1, si-TGF-β1 #2, si-TGF-β1 #3) and a negative control sequence (si-TGF-β1 NC) were synthesized separately by Sangon Biotech (China). .. The EPCs were seeded into culture plates, and when EPCs confluence reached 60% to 70%, transfection was performed using Lipofectamin 3000 reagent (Thermo Fisher Scientific).

Synthesized:

Article Title: Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane
Article Snippet: .. Three small interfering RNA sequences targeting TGF-β1 (si-TGF-β1 #1, si-TGF-β1 #2, si-TGF-β1 #3) and a negative control sequence (si-TGF-β1 NC) were synthesized separately by Sangon Biotech (China). .. The EPCs were seeded into culture plates, and when EPCs confluence reached 60% to 70%, transfection was performed using Lipofectamin 3000 reagent (Thermo Fisher Scientific).

Article Title: Centromere protein I promotes hepatocellular carcinoma progression by activating PI3K/AKT/mTOR-CDK2 cascade
Article Snippet: .. CENPI-targeting siRNAs and a nontargeting control siRNA (si-NC) were synthesized by Sangon Biotech (Shanghai, China). .. HepG2 cells were seeded in 6-well plates at 2 × 10 5 cells/well and transfected at 60%–70% confluence using Lipofectamine 8000 (Beyotime, China, catalog number C0533) in DMEM medium, according to the manufacturer's instructions.

Article Title: Extracellular vesicle-encapsulated miRNAs mediate monocyte-endothelial cell crosstalk to exacerbate renal injury in diabetic kidney disease.
Article Snippet: Background: Diabetic kidney disease (DKD) is a major microvascular complication of diabetes, with microcirculatory dysfunction and immune injury as its core pathological features.. Extracellular vesicles (EVs) act as key mediators of intercellular communication, but it remains unclear whether EV-encapsulated microRNAs (miRNAs) are involved in the crosstalk between endothelial cells (ECs) and monocytes in DKD.. Methods: EVs were isolated from monocytes and ECs under normal-glucose and high-glucose conditions.

Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

Article Title: Fisetin alleviates osteoporosis by promoting osteogenesis and suppressing adipogenesis via the Wnt/β-catenin signaling pathway in mice.
Article Snippet: Osteoporosis (OP) is characterized by an imbalance in which bone marrow mesenchymal stem cells (BMSCs) preferentially differentiate into adipocytes rather than osteoblasts.. The natural polyhydroxyflavone fisetin functions as an antioxidant and multi-target signaling regulator with potential to modulate stem cell fate.. However, its role and underlying mechanisms in OP remain unclear.

Transfection:

Article Title: Neuronal PRRT3 coordinates amyloidogenic processing and Tau phosphorylation via distinct Ras-ERK-AP-1 and CaMKII/PP2A pathways.
Article Snippet: .. For transfection, PRRT3-specific siRNA (si-PRRT3: 5′-GAGUUUCUCCUGGGAGCUUTT-3′) and negative control siRNA (si-Con: 5′-UUCUCCGAACGUGUCACGUTT-3′), purchased from Sangon Biotech (Shanghai, China), were separately mixed with transfection reagent (CALNPTM RNAi in vitro; DN001-05; D-Nano Therapeutics, Beijing, China) in Opti-MEM (31985070; Gibco) serum-free medium and left at room temperature to form complexes, with reagent amounts added according to the instructions. ..

Article Title: Single-cell multiomics gene regulatory landscape reveals impaired spermatogonial stem cells and macrophage-driven inflammaging during testicular aging.
Article Snippet: 29 Testicular aging is a key driver of declining male reproductive health, but a comprehensive 30 understanding of its underlying epigenetic drivers is lacking.. To address this, we construct a 31 multiomics aging atlas by integrating single-cell RNA sequencing, single-cell assay for 32 transposase-accessible chromatin sequencing (scATAC-seq), and spatial transcriptomics of 33 young and aged mouse testes.. Our analysis reveals that altered chromatin accessibility 34 accompanies transcriptional dysregulation and identifies spermatogonial stem cells (SSCs) as 35 the most epigenetically vulnerable population.

Article Title: Fisetin alleviates osteoporosis by promoting osteogenesis and suppressing adipogenesis via the Wnt/β-catenin signaling pathway in mice.
Article Snippet: Osteoporosis (OP) is characterized by an imbalance in which bone marrow mesenchymal stem cells (BMSCs) preferentially differentiate into adipocytes rather than osteoblasts.. The natural polyhydroxyflavone fisetin functions as an antioxidant and multi-target signaling regulator with potential to modulate stem cell fate.. However, its role and underlying mechanisms in OP remain unclear.

In Vitro:

Article Title: Neuronal PRRT3 coordinates amyloidogenic processing and Tau phosphorylation via distinct Ras-ERK-AP-1 and CaMKII/PP2A pathways.
Article Snippet: .. For transfection, PRRT3-specific siRNA (si-PRRT3: 5′-GAGUUUCUCCUGGGAGCUUTT-3′) and negative control siRNA (si-Con: 5′-UUCUCCGAACGUGUCACGUTT-3′), purchased from Sangon Biotech (Shanghai, China), were separately mixed with transfection reagent (CALNPTM RNAi in vitro; DN001-05; D-Nano Therapeutics, Beijing, China) in Opti-MEM (31985070; Gibco) serum-free medium and left at room temperature to form complexes, with reagent amounts added according to the instructions. ..

Article Title: Fisetin alleviates osteoporosis by promoting osteogenesis and suppressing adipogenesis via the Wnt/β-catenin signaling pathway in mice.
Article Snippet: Osteoporosis (OP) is characterized by an imbalance in which bone marrow mesenchymal stem cells (BMSCs) preferentially differentiate into adipocytes rather than osteoblasts.. The natural polyhydroxyflavone fisetin functions as an antioxidant and multi-target signaling regulator with potential to modulate stem cell fate.. However, its role and underlying mechanisms in OP remain unclear.



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KEY RESOURCES TABLE
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Image Search Results


Journal: Neuron

Article Title: Major Depressive Disorder associated dysregulation of ZBTB7A in orbitofrontal cortex promotes astrocyte-mediated stress susceptibility

doi: 10.1016/j.neuron.2025.05.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Negative control sequence without 5’ overhangs: GAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT , ThermoFisher , Cat # K493600.

Techniques: Virus, Generated, Cloning, Expressing, Recombinant, Blocking Assay, Plasmid Preparation, Negative Control, Sequencing, Software