negative control mir sequences (Shanghai GenePharma)

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Shanghai GenePharma negative control mir sequences

Upregulation of <t>miR-150</t> suppresses human papillary thyroid carcinoma cell proliferation and metastasis. (A) Relative miR-150 expression in TPC-1 cells following transfection. (B) Proliferation of TPC-1 cells following transfection. (C) Representative micrographs of a wound-healing assay in TPC-1 cells following transfection (Scale bars=50 µm). (D) Representative micrographs of Transwell invasion experiments in TPC-1 cells following transfection (Scale bars=50 µm). (E) Statistical analysis of the wound-healing assay results. (F) Statistical analysis of the Transwell invasion assay results. *P<0.05 vs. mimic-Con, **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, <t>negative</t> <t>control</t> transfection; OD, optical density.

Negative Control Mir Sequences, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+sequence/negative+control+mir+sequences/pmc05995047-33-4-12
Average 90 stars, based on 1 article reviews

negative control mir sequences - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4"

Article Title: MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4

Journal: Experimental and Therapeutic Medicine

doi: 10.3892/etm.2018.6197

Upregulation of miR-150 suppresses human papillary thyroid carcinoma cell proliferation and metastasis. (A) Relative miR-150 expression in TPC-1 cells following transfection. (B) Proliferation of TPC-1 cells following transfection. (C) Representative micrographs of a wound-healing assay in TPC-1 cells following transfection (Scale bars=50 µm). (D) Representative micrographs of Transwell invasion experiments in TPC-1 cells following transfection (Scale bars=50 µm). (E) Statistical analysis of the wound-healing assay results. (F) Statistical analysis of the Transwell invasion assay results. *P<0.05 vs. mimic-Con, **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; OD, optical density.

Figure Legend Snippet: Upregulation of miR-150 suppresses human papillary thyroid carcinoma cell proliferation and metastasis. (A) Relative miR-150 expression in TPC-1 cells following transfection. (B) Proliferation of TPC-1 cells following transfection. (C) Representative micrographs of a wound-healing assay in TPC-1 cells following transfection (Scale bars=50 µm). (D) Representative micrographs of Transwell invasion experiments in TPC-1 cells following transfection (Scale bars=50 µm). (E) Statistical analysis of the wound-healing assay results. (F) Statistical analysis of the Transwell invasion assay results. *P<0.05 vs. mimic-Con, **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; OD, optical density.

Techniques Used: Expressing, Transfection, Wound Healing Assay, Transwell Invasion Assay, Negative Control

MUC4 is a direct target of miR-150 in papillary thyroid carcinoma cells. (A) miR-150 and its target site in MUC4 3′-UTR. The mutant bases are indicated by boxes. (B) Luciferase reporter assay in TPC-1 cells transfected with reporter vectors containing WT or MUT MUC4 3′-UTR. (C) MUC4 mRNA expression was evaluated by reverse transcription-quantitative polymerase chain reaction in TPC-1 cells transfected with miR-150 mimics and mimic-Con. (D) MUC4 protein expression was evaluated by western blot analysis in TPC-1 cells transfected with miR-150 mimics and mimic-Con. (E) Densitometric analysis of MUC4 protein level. *P<0.05 vs. control; **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; MUC4, mucin 4; 3′-UTR, 3′-untranslated region; NC, negative control; WT, wild type; MUT, mutated.

Figure Legend Snippet: MUC4 is a direct target of miR-150 in papillary thyroid carcinoma cells. (A) miR-150 and its target site in MUC4 3′-UTR. The mutant bases are indicated by boxes. (B) Luciferase reporter assay in TPC-1 cells transfected with reporter vectors containing WT or MUT MUC4 3′-UTR. (C) MUC4 mRNA expression was evaluated by reverse transcription-quantitative polymerase chain reaction in TPC-1 cells transfected with miR-150 mimics and mimic-Con. (D) MUC4 protein expression was evaluated by western blot analysis in TPC-1 cells transfected with miR-150 mimics and mimic-Con. (E) Densitometric analysis of MUC4 protein level. *P<0.05 vs. control; **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; MUC4, mucin 4; 3′-UTR, 3′-untranslated region; NC, negative control; WT, wild type; MUT, mutated.

Techniques Used: Mutagenesis, Luciferase, Reporter Assay, Transfection, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control, Negative Control

Effect of miR-150 on HER2 and downstream FAK/ERK signaling. (A) Protein levels were evaluated by western blot analysis. (B) Densitometric analysis of each protein level. **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; HER2, human epidermal growth factor receptor 2; FAK, focal adhesion kinase; ERK, extracellular signal-regulated kinase.

Figure Legend Snippet: Effect of miR-150 on HER2 and downstream FAK/ERK signaling. (A) Protein levels were evaluated by western blot analysis. (B) Densitometric analysis of each protein level. **P<0.01 vs. mimic-Con. miR-150, microRNA-150; mimic-Con, negative control transfection; HER2, human epidermal growth factor receptor 2; FAK, focal adhesion kinase; ERK, extracellular signal-regulated kinase.

Techniques Used: Western Blot, Negative Control, Transfection

other:

Article Title: PEP06 polypeptide 30 exerts antitumour effect in colorectal carcinoma via inhibiting epithelial–mesenchymal transition
Article Snippet: MiR‐146b‐5p‐expressing lentiviruses (miR‐146b‐OE) and its negative control (miR‐Ctl) containing the GFP were packaged by Shanghai GenePharma Co., Ltd. SW620 cells (5 × 10 4 ) were seeded in six‐well plates and cultured to about 90% confluence.

Article Title: microRNA-155 silencing inhibits proliferation and migration and induces apoptosis by upregulating BACH1 in renal cancer cells.
Article Snippet: ACHN cells were transfected with antisense-miR-155 oligonucleotide (As-miR-155), a negative control (miR-CON) (Shanghai GenePharma Co, Ltd. China) or an empty vector (blank) using Lipofectamine 2000TM (Invitrogen) following 24 h of cell seeding.

Negative Control:

Article Title: MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4
Article Snippet: .. miR-150 mimics (5′-UCUCCCAACCCUUGUACCAGUG-3′) and negative control miR sequences (5′-CCGAAACCUCGGUUGAUUGCGG-3′) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). .. Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) was used to perform TPC-1 cell transfection, according to the manufacturer's protocol.

Article Title: MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4.
Article Snippet: .. Cell transfection. miR-150 mimics (5'-UCU CCC AAC CCU UGU ACC AGUG-3') and negative control miR sequences (5'-CCG AAA CCU CGG UUG AUU GCGG-3') were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). .. Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) was used to perform TPC-1 cell transfection, according to the manufacturer's protocol.

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negative control mir sequences (Shanghai GenePharma)

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