lentiviruses designed for overexpressing mettl16 and senp3 (Shanghai GenePharma)

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Shanghai GenePharma lentiviruses designed for overexpressing mettl16 and senp3
Lentiviruses Designed For Overexpressing Mettl16 And Senp3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+sequence/shrna+sequences+targeting+mettl16+and+ppar%CE%B3++and+non+targeting+negative+control++sh+nc+/pm39218945-61-11-29
Average 90 stars, based on 1 article reviews

lentiviruses designed for overexpressing mettl16 and senp3 - by Bioz Stars, 2026-11

90/100 stars

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Negative Control:

Article Title: Role of METTL16 in PPARγ methylation and osteogenic differentiation.
Article Snippet: .. Three sequences targeting METTL16 and PPARγ, along with a non-targeting negative control (sh-NC), were synthesized by GenePharma® (Shanghai, China). .. The lentivirus packaging system was established using the pLKO.1 lentiviral vector for gene silencing and the overexpression lentiviral vector LV-PDGFRA was constructed by Guangzhou RiboBio Co., Ltd. For lentivirus production, the packaged virus and target vector were transfected into 293 T cells using Lipofectamine 2000 (Thermo Fisher Scientific), achieving 80–90% transfection efficiency.

Synthesized:

Over Expression:

Article Title: m 6 A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression.
Article Snippet: NCM460, DLD-1, HCE8693, and Colo320 were maintained in RPMI-1640, RKO in MEM, HCT116 and HT29 in McCoy’s 5A, SW620, SW480 in Leibovitz’s L-15 medium that supplied with 10% FBS (BI, Israel) and 1% penicillin and streptomycin (Sigma, USA) and maintained in 37° C incubator with 5% CO2. .. METTL16 proteins siRNAs and overexpression vectors were obtained from GenePharma (Shanghai, China). .. Cells were transfected with indicated oligonucleotides using the Lipofectamine 2000 reagent (Thermo Fisher, USA) as per manufacturer’s protocol.

Knockdown:

Article Title: METTL16 regulates the mRNA stability of FBXO5 via m6A modification to facilitate the malignant behavior of breast cancer.
Article Snippet: The Lipofectamine 3000 (Invitrogen, USA) was applied to transfect these vectors into BC cells. .. For the METTL16 knockdown, METTL16 knockdown lentivirus (LV-sh-METTL16) and the matching negative lentivirus (LV-sh-NC) were established via GenePharma. .. The lentivirus was infected in BC cells and the infected cells were screened in DMEM with puromycin (3 μg/mL, SigmaAldrich) for 14 d to construct the stably cell of METTL16 knockdown.

shRNA:

Article Title: Investigating the mechanism of METTL16-dependent m6A modification regulating the SAMD11 protein signaling pathway to inhibit thyroid cancer phenotypes.
Article Snippet: Despite substantial progress in the research and treatment of thyroid cancer, many areas in the molecular mechanisms remain to be explored.. This study aims to comprehensively and deeply investigate the key role and potential molecular mechanisms of RNA methyltransferase METTL16 in the development and progression of thyroid cancer.. Firstly, through bioinformatics analysis of tumor databases, we examined the correlation between METTL16 expression levels and patient prognosis.

Control:

other:

Article Title: m 6 A modification of AC026356.1 facilitates hepatocellular carcinoma progression by regulating the IGF2BP1-IL11 axis.
Article Snippet: RNA immunoprecipitation (RIP) and methylated RNA immunoprecipitation (MeRIP) assays To enrich m6A-modified transcripts, MeRIP assays were performed in indicated tissues and cells using the Magna MeRIP m6A Kit (cat. no. 17-10,499, Millipore, Billerica, MA, USA).

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