nectin4 (Proteintech)
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94
Structured Review
Proteintech
nectin4
Nectin4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nectin4/PVRL4+Antibody/pmc12753226-159-52-54
Average 94 stars, based on 6 article reviews
Nectin4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nectin4/PVRL4+Antibody/pmc12753226-159-52-54
Average 94 stars, based on 6 article reviews
nectin4 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Integrin β4 drives immune evasion and therapeutic resistance to PD-1 blockade in bladder cancer via MEK/ERK signaling"
Article Title: Integrin β4 drives immune evasion and therapeutic resistance to PD-1 blockade in bladder cancer via MEK/ERK signaling
Journal: The Journal of Biological Chemistry
doi: 10.1016/j.jbc.2025.110941
Figure Legend Snippet: ITGB4 positively correlated with enfortumab vedotin target NECTIN4. A , the RNA-Seq analysis in the GSE268855 dataset after overexpressing ITGB4 ; B , the correlation analysis in the DepMap dataset. p values are shown in the figure. C , Pan-cancer data analysis in the DepMap database revealed the correlation between ITGB4 and NECTIN4 . p values were shown in the figure. D – G , HT-1376 and 5637 cells were transfected with shITGB4 or shControl for 72 h. The harvested cells were used for Western blot analysis ( D ) and RT-qPCR detection ( E – G ). H – K , HT-1376 and 5637 cells were transfected with overexpressed ITGB4 plasmid or empty vector for 72 h, and harvested cells were used for Western blot analysis ( H ) and RT-qPCR detection ( I–K ). Data presents as mean ± SD with three replicates. ns, not significant; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001. L , the tissue microarray of bladder cancer stained with ITGB4 and NECTIN4, respectively. The typical IHC images stained with ITGB4 and NECTIN4 are shown in the panel . M , the correlation of these two proteins is shown in the panel , and the p -value is indicated in the figure.
Techniques Used: RNA Sequencing, Transfection, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Microarray, Staining
Figure Legend Snippet: ITGB4 plays a vital role in BLCA sequential therapies. A mechanism diagram depicting that the highly expressed ITGB4 in cisplatin-resistant BLCA activated the MEK/ERK pathway through tyrosine-1510 phosphorylation, upregulated the expression level of PD-L1 in BLCA, and caused anti-PD-1 treatment resistance. Meanwhile, the overexpressed ITGB4 was positively correlated to NECTIN4, promoting the sensitivity of BLCA to enfortumab vedotin treatment.
Techniques Used: Phospho-proteomics, Expressing
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Incubation:Article Title: METTL3-mediated m 6 A modification facilitates Nectin-4-induced VNN1 upregulation and promotion of ESCC progression. Article Snippet: The proteins were separated by 10% SDS-PAGE and transferred onto PVDF membranes (Epizyme). .. The membranes were blocked with 5% nonfat milk for 1 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: GAPDH (10494-1-AP, 1:10,000; Proteintech), METTL3 (15073-1- AP, 1:1000; Proteintech), Article Title: METTL3-mediated m 6 A modification facilitates Nectin-4-induced VNN1 upregulation and promotion of ESCC progression Article Snippet: The proteins were separated by 10% SDS-PAGE and transferred onto PVDF membranes (Epizyme). .. The membranes were blocked with 5% nonfat milk for 1 h at room temperature, followed by overnight incubation at 4°C with primary antibodies: GAPDH (10494-1-AP, 1:10,000; Proteintech), METTL3 (15073-1-AP, 1:1000; Proteintech), Immunohistochemistry:Article Title: Integrin β4 Drives Immune Evasion and Therapeutic Resistance to PD-1 blockade in Bladder Cancer via MEK/ERK Signaling. Article Snippet: ImageJ software was used to evaluate the protein levels, and then plotted them into bar charts through Graphpad Prism 9. .. Immunohistochemistry was performed with primary antibodies against ITGB4 (#21738-1-AP, Proteintech, 1:300 dilution), Article Title: Integrin β4 drives immune evasion and therapeutic resistance to PD-1 blockade in bladder cancer via MEK/ERK signaling Article Snippet: ImageJ software was used to evaluate the protein levels, and then plotted them into bar charts through Graphpad Prism 9. .. Immunohistochemistry was performed with primary antibodies against ITGB4 (#21738-1-AP, Proteintech, 1:300 dilution), Virus:Article Title: Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion Article Snippet: Wheat Germ Agglutinin (WGA, Red, Invitrogen, W11262) and pHrodo Red dextran (Invitrogen, P10361) were purchased from Invitrogen. .. Rabbit anti-GAPDH (CST, 5174 S), other:Article Title: Integrin β4 drives immune evasion and therapeutic resistance to PD-1 blockade in bladder cancer via MEK/ERK signaling Article Snippet: The primary antibodies used were as follows: |