buffer 3 (New England Biolabs)

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New England Biolabs buffer 3
Buffer 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuffer+2+1+buffer/NEBuffer+3/pm41955813-97-24-26
Average 97 stars, based on 1150 article reviews

buffer 3 - by Bioz Stars, 2026-09

97/100 stars

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Purification:

Article Title: Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase From Schistosoma mansoni
Article Snippet: .. Bsu36I digestion a. Digest 10 × 6 μg aliquots of the bacmid each in 100 μL final volume containing 10 μL 10× NEBuffer TM 3 and 1 μL 100× BSA 1 μL Bsu36I. b. Incubate for 2 h at 37 °C. c. Add another 1 μL of Bsu36I per 6 μg of DNA. d. Incubate another 2–3 h at 37 °C. e. Heat at 72 °C for 20 min. No additional purification of the bacmid is required. f. Divide into 10 μL aliquots in PCR 8-well strip tubes and store the cut bacmid at -70 °C. ..

Polymerase Chain Reaction:

Article Title: Hypomorphic Protein Expression of DNA Polymerase Beta in Polβ L301R-V303R/L301R-V303R Knock-In Transgenic Mice Does Not Impact Global DNA Methylation Levels in the Midbrain.
Article Snippet: .. A total of 12 μL of each PCR amplicon was then digested with 0.8 μL XcmI Restriction Endonuclease (NEB), 3 μL NEBuffer Restriction Digest Buffer (NEB), and 15 μL qRT–PCR grade water (Invitrogen) by incubation at 37 ◦C for 50 min followed by a 5 min digestion at 85 ◦C. .. The samples were then separated by electrophoresis on a 2% agarose gel [2g SeaKem LE Agarose (Lonza, Lexington, MA, USA), 100 mL 1X Tris-Borate-EDTA (TBE) Buffer (Thermo Scientific), supplemented with ethidium bromide (0.5 μg/mL)] and electrophoresed in 1X TBE buffer (Thermo Scientific).

Article Title: Hypomorphic Protein Expression of DNA Polymerase Beta in Polβ L301R-V303R/L301R-V303R Knock-In Transgenic Mice Does Not Impact Global DNA Methylation Levels in the Midbrain
Article Snippet: .. A total of 12 μL of each PCR amplicon was then digested with 0.8 μL XcmI Restriction Endonuclease (NEB), 3 μL NEBuffer Restriction Digest Buffer (NEB), and 15 μL qRT–PCR grade water (Invitrogen) by incubation at 37 °C for 50 min followed by a 5 min digestion at 85 °C. .. The samples were then separated by electrophoresis on a 2% agarose gel [2g SeaKem LE Agarose (Lonza, Lexington, MA, USA), 100 mL 1X Tris-Borate-EDTA (TBE) Buffer (Thermo Scientific), supplemented with ethidium bromide (0.5 μg/mL)] and electrophoresed in 1X TBE buffer (Thermo Scientific).

Stripping Membranes:

Amplification:

Article Title: Hypomorphic Protein Expression of DNA Polymerase Beta in Polβ L301R-V303R/L301R-V303R Knock-In Transgenic Mice Does Not Impact Global DNA Methylation Levels in the Midbrain
Article Snippet: .. A total of 12 μL of each PCR amplicon was then digested with 0.8 μL XcmI Restriction Endonuclease (NEB), 3 μL NEBuffer Restriction Digest Buffer (NEB), and 15 μL qRT–PCR grade water (Invitrogen) by incubation at 37 °C for 50 min followed by a 5 min digestion at 85 °C. .. The samples were then separated by electrophoresis on a 2% agarose gel [2g SeaKem LE Agarose (Lonza, Lexington, MA, USA), 100 mL 1X Tris-Borate-EDTA (TBE) Buffer (Thermo Scientific), supplemented with ethidium bromide (0.5 μg/mL)] and electrophoresed in 1X TBE buffer (Thermo Scientific).

Incubation:

Article Title: Hypomorphic Protein Expression of DNA Polymerase Beta in Polβ L301R-V303R/L301R-V303R Knock-In Transgenic Mice Does Not Impact Global DNA Methylation Levels in the Midbrain
Article Snippet: .. A total of 12 μL of each PCR amplicon was then digested with 0.8 μL XcmI Restriction Endonuclease (NEB), 3 μL NEBuffer Restriction Digest Buffer (NEB), and 15 μL qRT–PCR grade water (Invitrogen) by incubation at 37 °C for 50 min followed by a 5 min digestion at 85 °C. .. The samples were then separated by electrophoresis on a 2% agarose gel [2g SeaKem LE Agarose (Lonza, Lexington, MA, USA), 100 mL 1X Tris-Borate-EDTA (TBE) Buffer (Thermo Scientific), supplemented with ethidium bromide (0.5 μg/mL)] and electrophoresed in 1X TBE buffer (Thermo Scientific).

Ligation:

Article Title: Hierarchical TBX6-FOXC Regulatory Logic Controls Human Trunk Mesoderm Diversification
Article Snippet: .. For ligation-based combinatorial barcoding the pooled nuclei were resuspended in 1 ml of 1x NEBuffer3.1 (NEB) and transferred to 2,910 μl of ligation buffer (1x T4 ligase buffer (NEB), 0.35mg/ml BSA, 25 mM NaCl 2 , 40,000U T4 ligase (NEB), and 0.25 U/μl ea Superase RNAse Inhibitor and RNAaseOUT). ..

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