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buffer 2 1  (New England Biolabs)


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    Structured Review

    New England Biolabs buffer 2 1
    Buffer 2 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebuffer+2+1+buffer/NEBuffer+2%2E1/pm31932773-231-35-37
    Average 97 stars, based on 177 article reviews
    buffer 2 1 - by Bioz Stars, 2026-09
    97/100 stars

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    other:

    Article Title: HIPSD&R-seq enables scalable genomic copy number and transcriptome profiling
    Article Snippet: PrePrint: 10 mM NaCl, 3 mM MgCl2, 0.1% igepal, 1x protease inhibitor cocktail (#5871S, Cell Signaling Technology)) and incubated on ice for 20 min with gentle mixing. Nuclei were centrifuged (500xg, 5 min, 4°C) and washed once with 1X NEBuffer 2.1 (NEB, #B7202). For nucleosome depletion, nuclei were resuspended in 1X NEBuffer 2.1 supplemented with 0.3% SDS (Serva, #20767) and incubated at 42°C, for 15 min with shaking. SDS was quenched by adding 2% Triton-



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