gene exp gclc rn00563101 m1 (Thermo Fisher)
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gene exp gclc rn00563101 m1
Gene Exp Gclc Rn00563101 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncbi+refseq+transcripts/pmc03547088-108-34--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
Gene Exp Gclc Rn00563101 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncbi+refseq+transcripts/pmc03547088-108-34--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp gclc rn00563101 m1 - by Bioz Stars,
2026-08
85/100 stars
Images
1) Product Images from "Age-Specific Effects on Rat Lung Glutathione and Antioxidant Enzymes after Inhaling Ultrafine Soot"
Article Title: Age-Specific Effects on Rat Lung Glutathione and Antioxidant Enzymes after Inhaling Ultrafine Soot
Journal: American Journal of Respiratory Cell and Molecular Biology
doi: 10.1165/rcmb.2012-0108OC
Figure Legend Snippet: TAQMAN GENE EXPRESSION ASSAY CATALOG NUMBERS
Techniques Used: Gene Expression
Figure Legend Snippet: GCLc and GCLm gene expression. RT-PCR expression in airway and parenchyma compartments in neonates and adult rats exposed to PFPs. Basal GCLc was consistently expressed in higher abundance than GCLm, and its highest expression was seen in adult airways (A). After PFP exposure, a transient drop in neonatal airway GCLc expression was observed in PFP24 compared with PFP2 (B). No treatment effects were detected in adult animals (C). Data are plotted as means ± SEM (n = 5–7 rats per group, per compartment, per gene). P < 0.05 are denoted as follows: *significantly different from neonates in the same compartment, and †significantly different from airways in the same age. PFP2, PFP24, and PFP48 refer to PFP exposure for 4, 24, and 48 hours, respectively.
Techniques Used: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Expressing
Figure Legend Snippet: GCLc and GCLm protein analysis through Western blotting and immunohistochemistry. Representative GCLc and GCLm Western blots with actin loading control (A). GCLc/m Western blots were quantified: while neonatal GCLc/m expression remained unchanged after exposure (B), a significant up-regulation in GCLc was detected in PFP2 adult rats compared against FA controls (C). Data are plotted as means ± SEM (n = 6 rats per group). P < 0.05 is denoted as follows: ‡significantly different from FA in the same age. GCL immunohistochemical images in neonatal (D–G) and adult (H–K) rats reared in FA (D and H) and exposed to PFP: PFP2 (E and I), PFP24 (F and J), and PFP48 (G and K). Intense GCL staining was observed in adult and neonatale PFP2. In contrast to neonates, staining in adult PFP48 was continually up-regulated. High magnification inserts highlight GCL-positive cells in treated groups. Scale bars for D–K (shown in K) are 50 μm.
Techniques Used: Western Blot, Immunohistochemistry, Control, Expressing, Immunohistochemical staining, Staining