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ncbi reference sequence nm 031476  (OriGene)


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    OriGene ncbi reference sequence nm 031476
    Ncbi Reference Sequence Nm 031476, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncbi+refseq+plasmid+database/CRISPLD2+(NM_031476)+Human+Tagged+ORF+Clone/pmc06258193-104-9-16
    Average 90 stars, based on 2 article reviews
    ncbi reference sequence nm 031476 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Crispld2 is Required for Neural Crest Cell Migration and Cell Viability During Zebrafish Craniofacial Development
    Article Snippet: .. A plasmid harboring the full-length human CRISPLD2 cDNA representing NCBI reference sequence NM_031476 was purchased from OriGene (#RC214912, OriGene Technologies.) .. The full length CRISPLD2 cDNA was subcloned into the pCS2 vector and CRISPLD2 mRNA was generated using the mMessage mMachine Sp6 kit (Ambion). mRNA was resuspended in nuclease-free water to 500 μg/mL stock concentration and diluted to 50 μg/mL in Danieu buffer for injection.

    Sequencing:

    Article Title: Crispld2 is Required for Neural Crest Cell Migration and Cell Viability During Zebrafish Craniofacial Development
    Article Snippet: .. A plasmid harboring the full-length human CRISPLD2 cDNA representing NCBI reference sequence NM_031476 was purchased from OriGene (#RC214912, OriGene Technologies.) .. The full length CRISPLD2 cDNA was subcloned into the pCS2 vector and CRISPLD2 mRNA was generated using the mMessage mMachine Sp6 kit (Ambion). mRNA was resuspended in nuclease-free water to 500 μg/mL stock concentration and diluted to 50 μg/mL in Danieu buffer for injection.

    other:

    Article Title: Crispld2 is Required for Neural Crest Cell Migration and Cell Viability During Zebrafish Craniofacial Development
    Article Snippet: Alexa Fluor 488 donkey anti-rabbit IgG (# A21206 , LifeTechnologies, Eugene) was used as secondary antibody at 1:500 dilution in blocking solution and 1 h incubation at room temperature.



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    Image Search Results


    The plasmid host distribution in NCBI validation dataset. The validation dataset was dominated by the Enterobacterales, Lactobacillales and Pseudomonadales, which make up 76% of the NCBI plasmid hosts.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: The plasmid host distribution in NCBI validation dataset. The validation dataset was dominated by the Enterobacterales, Lactobacillales and Pseudomonadales, which make up 76% of the NCBI plasmid hosts.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: Plasmid Preparation, Biomarker Discovery

    Model accuracy for the NCBI plasmids tested with the whole model that was trained with the PATRIC dataset. Each bar shows the number of bacterial orders in the validation data and corresponding model accuracy was color coded. The plot showed that the accuracy of the models changed roughly according to the availability of the host organisms in the training data which was indicated on top of the bars.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: Model accuracy for the NCBI plasmids tested with the whole model that was trained with the PATRIC dataset. Each bar shows the number of bacterial orders in the validation data and corresponding model accuracy was color coded. The plot showed that the accuracy of the models changed roughly according to the availability of the host organisms in the training data which was indicated on top of the bars.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: Biomarker Discovery

    The fragment models were validated with the NCBI plasmids. The fragments models that trained with the 500, 1,000 and 1,500 nucleotide (nt) fragments from the PATRIC plasmids were validated with the fragments that sub-sampled from the NCBI plasmids. Similar to the hold-out results, the best performance was obtained with the 1,500 nucleotide fragments and 8 -mers.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: The fragment models were validated with the NCBI plasmids. The fragments models that trained with the 500, 1,000 and 1,500 nucleotide (nt) fragments from the PATRIC plasmids were validated with the fragments that sub-sampled from the NCBI plasmids. Similar to the hold-out results, the best performance was obtained with the 1,500 nucleotide fragments and 8 -mers.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: