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human irx2  (OriGene)


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    Structured Review

    OriGene human irx2
    Figure 1. Increased expression of <t>IRX2</t> was observed in osteosarcoma tissue samples, determined using western blot analysis. (A) Results of western blot analysis for the expression of IRX2 in eight paired osteosarcoma and corresponding normal tissue samples. (B) Proteins were extracted from primary and metastatic tissue samples. β‑actin was used as a loading control. N, normal; P, paired osteosarcoma.
    Human Irx2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncbi+refseq+plasmid+database/IRX2+(NM_033267)+Human+Tagged+ORF+Clone/pm26062523-48-4-10
    Average 90 stars, based on 1 article reviews
    human irx2 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner."

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.

    Journal: Molecular medicine reports

    doi: 10.3892/mmr.2015.3915

    Figure 1. Increased expression of IRX2 was observed in osteosarcoma tissue samples, determined using western blot analysis. (A) Results of western blot analysis for the expression of IRX2 in eight paired osteosarcoma and corresponding normal tissue samples. (B) Proteins were extracted from primary and metastatic tissue samples. β‑actin was used as a loading control. N, normal; P, paired osteosarcoma.
    Figure Legend Snippet: Figure 1. Increased expression of IRX2 was observed in osteosarcoma tissue samples, determined using western blot analysis. (A) Results of western blot analysis for the expression of IRX2 in eight paired osteosarcoma and corresponding normal tissue samples. (B) Proteins were extracted from primary and metastatic tissue samples. β‑actin was used as a loading control. N, normal; P, paired osteosarcoma.

    Techniques Used: Expressing, Western Blot, Control

    Figure 3. IRX2 induces the phosphorylation of Akt and increases the expression levels of MMP‑9 and VEGF in osteosarcoma cells. Western blot analysis of the protein expression of VEGF, MMP‑9, p‑AKT and AKT in the indicated cells. β‑actin was used as an internal control. p‑, phosphorylated.
    Figure Legend Snippet: Figure 3. IRX2 induces the phosphorylation of Akt and increases the expression levels of MMP‑9 and VEGF in osteosarcoma cells. Western blot analysis of the protein expression of VEGF, MMP‑9, p‑AKT and AKT in the indicated cells. β‑actin was used as an internal control. p‑, phosphorylated.

    Techniques Used: Phospho-proteomics, Expressing, Western Blot, Control

    Figure 2. Increased expression of IRX2 promotes osteosarcoma cell growth and invasion in vitro. (A and B) Protein and mRNA expression levels of IRX2 were determined in MG63 and SaOS2 osteosarcoma cells transfected with IRX2 or a control vector. (C) An MTT assay was performed to assess the cell growth of MG63 and SaOS2 cells transfected with IRX2 or the control vector. (D) Representative images of the invasion assay of IRX2‑transfected osteosarcoma cells. The MG63 and SaOS2 cells were transfected with IRX2 and exhibited increased invasive activity, compared with the control vector‑transfected cells (magnification, x100). Quantitative analysis of cell invasion was assessed following 48 h of chemo‑attraction. Values represent the mean ± standard deviation of triplicates in one representative experiment. *P<0.05, compared with the control vector. OD, optical density.
    Figure Legend Snippet: Figure 2. Increased expression of IRX2 promotes osteosarcoma cell growth and invasion in vitro. (A and B) Protein and mRNA expression levels of IRX2 were determined in MG63 and SaOS2 osteosarcoma cells transfected with IRX2 or a control vector. (C) An MTT assay was performed to assess the cell growth of MG63 and SaOS2 cells transfected with IRX2 or the control vector. (D) Representative images of the invasion assay of IRX2‑transfected osteosarcoma cells. The MG63 and SaOS2 cells were transfected with IRX2 and exhibited increased invasive activity, compared with the control vector‑transfected cells (magnification, x100). Quantitative analysis of cell invasion was assessed following 48 h of chemo‑attraction. Values represent the mean ± standard deviation of triplicates in one representative experiment. *P<0.05, compared with the control vector. OD, optical density.

    Techniques Used: Expressing, In Vitro, Transfection, Control, Plasmid Preparation, MTT Assay, Invasion Assay, Activity Assay, Standard Deviation

    Figure 4. PI3K/AKT are involved in IRX2‑induced cell proliferation, invasion and upregulation of MMP‑9 and VEGF. (A) Effects of Ly294002 on cell proliferation were measured using an MTT assay in MG63 and SaOS2 cells transfected with IRX2. (B) Effects of Ly294002 on cell invasion were assessed using an invasion assay in MG63 and SaOS2 cells transfected with IRX2. *P<0.05 and **P<0.01, vs. DMSO control. (C) MG63 and SaOS2 cells transfected with IRX2 were treated with or without Ly294002, and the expression levels of VEGF, MMP‑9, p‑Akt and AKT were detected using western blot analysis. p‑, phosphorylated. DMSO, dimethyl sulfoxide.
    Figure Legend Snippet: Figure 4. PI3K/AKT are involved in IRX2‑induced cell proliferation, invasion and upregulation of MMP‑9 and VEGF. (A) Effects of Ly294002 on cell proliferation were measured using an MTT assay in MG63 and SaOS2 cells transfected with IRX2. (B) Effects of Ly294002 on cell invasion were assessed using an invasion assay in MG63 and SaOS2 cells transfected with IRX2. *P<0.05 and **P<0.01, vs. DMSO control. (C) MG63 and SaOS2 cells transfected with IRX2 were treated with or without Ly294002, and the expression levels of VEGF, MMP‑9, p‑Akt and AKT were detected using western blot analysis. p‑, phosphorylated. DMSO, dimethyl sulfoxide.

    Techniques Used: MTT Assay, Transfection, Invasion Assay, Control, Expressing, Western Blot

    Related Articles

    Sequencing:

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).

    Amplification:

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).

    Polymerase Chain Reaction:

    Article Title: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner.
    Article Snippet: .. The coding sequence of human IRX2 (NM_033267.4; NCBI Reference Sequence, OriGene Technologies, Rockville, MD, USA) was amplified by performing polymerase chain reaction (PCR) using the forward pr imer, 5'-GGATCCATGTCCTACCCGCAGGGC-3', which introduced a BamHI site, and the reverse primer, 5'-GAATTCCTATAGGTAGGGCTGGACGC-3' (Sangon Biotech Co., Ltd., Shanghai, China), which introduced an EcoRI site. .. The PCR conditions were as follows: Initial denaturation at 98 ̊C for 2 min, followed by 30 cycles of denaturation at 98 ̊C for 15 sec, primer annealing at 55 ̊C for 15 sec, and a primer extension step at 72 ̊C for 2 min, using high‐fidelity polymerase PrimeStar (Takara Bio Inc., Otsu, Japan).



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    Image Search Results


    The plasmid host distribution in NCBI validation dataset. The validation dataset was dominated by the Enterobacterales, Lactobacillales and Pseudomonadales, which make up 76% of the NCBI plasmid hosts.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: The plasmid host distribution in NCBI validation dataset. The validation dataset was dominated by the Enterobacterales, Lactobacillales and Pseudomonadales, which make up 76% of the NCBI plasmid hosts.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: Plasmid Preparation, Biomarker Discovery

    Model accuracy for the NCBI plasmids tested with the whole model that was trained with the PATRIC dataset. Each bar shows the number of bacterial orders in the validation data and corresponding model accuracy was color coded. The plot showed that the accuracy of the models changed roughly according to the availability of the host organisms in the training data which was indicated on top of the bars.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: Model accuracy for the NCBI plasmids tested with the whole model that was trained with the PATRIC dataset. Each bar shows the number of bacterial orders in the validation data and corresponding model accuracy was color coded. The plot showed that the accuracy of the models changed roughly according to the availability of the host organisms in the training data which was indicated on top of the bars.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: Biomarker Discovery

    The fragment models were validated with the NCBI plasmids. The fragments models that trained with the 500, 1,000 and 1,500 nucleotide (nt) fragments from the PATRIC plasmids were validated with the fragments that sub-sampled from the NCBI plasmids. Similar to the hold-out results, the best performance was obtained with the 1,500 nucleotide fragments and 8 -mers.

    Journal: bioRxiv

    Article Title: PlasmidHostFinder: Prediction of plasmid hosts using random forest

    doi: 10.1101/2021.09.27.462084

    Figure Lengend Snippet: The fragment models were validated with the NCBI plasmids. The fragments models that trained with the 500, 1,000 and 1,500 nucleotide (nt) fragments from the PATRIC plasmids were validated with the fragments that sub-sampled from the NCBI plasmids. Similar to the hold-out results, the best performance was obtained with the 1,500 nucleotide fragments and 8 -mers.

    Article Snippet: The plasmid host models that were trained with the PATRIC plasmids at four different taxonomic levels were validated using plasmids from the National Center for Biotechnology Information (NCBI) Reference Sequence database (RefSeq) ( ).

    Techniques: