other:Article Title:
Article Snippet: Rabbit polyclonal anti-Aurora A , Novus Biologicals , NB100-267, RRID:AB_10002481.
Recombinant:Article Title: Chromosome Segregation Is Biased by Kinetochore Size
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human anti - centromere (CREST) Fitzgerald Cat#90C-CS1058 Mouse anti - a-tubulin (clone B512) Sigma-Aldrich Cat#T5168 Mouse anti - CENP-A [22] Lars E.T. .. Jansen Rabbit anti - Hec1 Abcam Cat#ab3613 Mouse anti - cMad2 Santa Cruz Cat#sc-65492 Rabbit anti - Mad2: Bethyl Laboratories Cat#A300-301A Rabbit anti - pAuroraB, (pT232) Rockland Cat#600-401-677 Rabbit anti – AuroraA Novus Biologicals CatNB100-267 Mouse anti – GAPDH Proteintech Cat#60004-1-Ig Rabbit anti - phospho-histone H3 (S10) Cell Signaling Cat#3377 Rabbit anti – survivin Novus Biologicals Cat#NB500-201 Rabbit anti - pKNL1 (clone 58A) [31] Iain Cheeseman Sheep anti - CENP-E [56] William C. Earnshaw Rabbit anti - kif4a Thermo Fisher Scientific Cat#pa5-30492 Abberior donkey anti-human IgG STAR 580 Abberior Instruments Cat#D-08-2015Hp Abberior goat anti- mouse IgG STAR 635p Abberior Instruments Cat#S-11-2015Hp SiR-Tubulin Spirochrome Cat#CY-SC002 Chemicals, Peptides, and Recombinant Proteins Monastrol Tocris bioscience Cat#1305 ZM447439 (Aurora B inhibitor) Selleckchem.com Cat#S1103 MNL8054 (Aurora A inhibitor) Selleck Cat#869363-13-3 AZ 3146 (Mps1 inhibitor) Tocris bioscience Cat#3994 GSK923295 (CENP-E inhibitor) Selleckchem.com Cat#S7090 BSA 98% Sigma-Aldrich Cat#A3294-50G Experimental Models: Cell Lines Immortalized female Indian muntjac fibroblasts [29] Jerry W Shay Indian muntjac- H2B-GFP (female) In this paper N/A Indian muntjac- CENP-A-GFP (female) In this paper N/A Oligonucleotides 50-GCAGACATTGAGAGAATAA-30 (Ndc80) Sigma-Aldrich N/A 50-GCAGAATGGTTATACAAGT-30 (Mad2) Sigma-Aldrich N/A 50-GCTGCTGTTGCTGATGCTT-30 (CLASP1) Sigma-Aldrich N/A 50-GCGTCTCCACGTTTAAGAA-30 (Survivin) Sigma-Aldrich N/A 50-GAACCGTCAGCAAGACAA-30 (Kif4a) Sigma-Aldrich N/A 50-GCCAGGGACCTCATTTCAA-30 (Aurora A) Sigma-Aldrich N/A Recombinant DNA H2B-GFP Addgene plasmid # 11680 Geoff Wahl lab pSV-IRESneo3-CENP-A-EGFP [57] Patrick Meraldi Software and Algorithms Fiji/ImageJ ImageJ N/A CellProfiler [58] CellProfiler (http://cellprofiler.org) CellProfilerAnalyzer [58] CellProfiler (http://cellprofiler.org) MatLab8.1 The MathWorks https://www.mathworks.com/products/matlab.html Current Biology 28, 1344–1356.e1–e5, May 7, 2018 e1 ..
Plasmid Preparation:Article Title: Chromosome Segregation Is Biased by Kinetochore Size
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human anti - centromere (CREST) Fitzgerald Cat#90C-CS1058 Mouse anti - a-tubulin (clone B512) Sigma-Aldrich Cat#T5168 Mouse anti - CENP-A [22] Lars E.T. .. Jansen Rabbit anti - Hec1 Abcam Cat#ab3613 Mouse anti - cMad2 Santa Cruz Cat#sc-65492 Rabbit anti - Mad2: Bethyl Laboratories Cat#A300-301A Rabbit anti - pAuroraB, (pT232) Rockland Cat#600-401-677 Rabbit anti – AuroraA Novus Biologicals CatNB100-267 Mouse anti – GAPDH Proteintech Cat#60004-1-Ig Rabbit anti - phospho-histone H3 (S10) Cell Signaling Cat#3377 Rabbit anti – survivin Novus Biologicals Cat#NB500-201 Rabbit anti - pKNL1 (clone 58A) [31] Iain Cheeseman Sheep anti - CENP-E [56] William C. Earnshaw Rabbit anti - kif4a Thermo Fisher Scientific Cat#pa5-30492 Abberior donkey anti-human IgG STAR 580 Abberior Instruments Cat#D-08-2015Hp Abberior goat anti- mouse IgG STAR 635p Abberior Instruments Cat#S-11-2015Hp SiR-Tubulin Spirochrome Cat#CY-SC002 Chemicals, Peptides, and Recombinant Proteins Monastrol Tocris bioscience Cat#1305 ZM447439 (Aurora B inhibitor) Selleckchem.com Cat#S1103 MNL8054 (Aurora A inhibitor) Selleck Cat#869363-13-3 AZ 3146 (Mps1 inhibitor) Tocris bioscience Cat#3994 GSK923295 (CENP-E inhibitor) Selleckchem.com Cat#S7090 BSA 98% Sigma-Aldrich Cat#A3294-50G Experimental Models: Cell Lines Immortalized female Indian muntjac fibroblasts [29] Jerry W Shay Indian muntjac- H2B-GFP (female) In this paper N/A Indian muntjac- CENP-A-GFP (female) In this paper N/A Oligonucleotides 50-GCAGACATTGAGAGAATAA-30 (Ndc80) Sigma-Aldrich N/A 50-GCAGAATGGTTATACAAGT-30 (Mad2) Sigma-Aldrich N/A 50-GCTGCTGTTGCTGATGCTT-30 (CLASP1) Sigma-Aldrich N/A 50-GCGTCTCCACGTTTAAGAA-30 (Survivin) Sigma-Aldrich N/A 50-GAACCGTCAGCAAGACAA-30 (Kif4a) Sigma-Aldrich N/A 50-GCCAGGGACCTCATTTCAA-30 (Aurora A) Sigma-Aldrich N/A Recombinant DNA H2B-GFP Addgene plasmid # 11680 Geoff Wahl lab pSV-IRESneo3-CENP-A-EGFP [57] Patrick Meraldi Software and Algorithms Fiji/ImageJ ImageJ N/A CellProfiler [58] CellProfiler (http://cellprofiler.org) CellProfilerAnalyzer [58] CellProfiler (http://cellprofiler.org) MatLab8.1 The MathWorks https://www.mathworks.com/products/matlab.html Current Biology 28, 1344–1356.e1–e5, May 7, 2018 e1 ..
Software:Article Title: Chromosome Segregation Is Biased by Kinetochore Size
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human anti - centromere (CREST) Fitzgerald Cat#90C-CS1058 Mouse anti - a-tubulin (clone B512) Sigma-Aldrich Cat#T5168 Mouse anti - CENP-A [22] Lars E.T. .. Jansen Rabbit anti - Hec1 Abcam Cat#ab3613 Mouse anti - cMad2 Santa Cruz Cat#sc-65492 Rabbit anti - Mad2: Bethyl Laboratories Cat#A300-301A Rabbit anti - pAuroraB, (pT232) Rockland Cat#600-401-677 Rabbit anti – AuroraA Novus Biologicals CatNB100-267 Mouse anti – GAPDH Proteintech Cat#60004-1-Ig Rabbit anti - phospho-histone H3 (S10) Cell Signaling Cat#3377 Rabbit anti – survivin Novus Biologicals Cat#NB500-201 Rabbit anti - pKNL1 (clone 58A) [31] Iain Cheeseman Sheep anti - CENP-E [56] William C. Earnshaw Rabbit anti - kif4a Thermo Fisher Scientific Cat#pa5-30492 Abberior donkey anti-human IgG STAR 580 Abberior Instruments Cat#D-08-2015Hp Abberior goat anti- mouse IgG STAR 635p Abberior Instruments Cat#S-11-2015Hp SiR-Tubulin Spirochrome Cat#CY-SC002 Chemicals, Peptides, and Recombinant Proteins Monastrol Tocris bioscience Cat#1305 ZM447439 (Aurora B inhibitor) Selleckchem.com Cat#S1103 MNL8054 (Aurora A inhibitor) Selleck Cat#869363-13-3 AZ 3146 (Mps1 inhibitor) Tocris bioscience Cat#3994 GSK923295 (CENP-E inhibitor) Selleckchem.com Cat#S7090 BSA 98% Sigma-Aldrich Cat#A3294-50G Experimental Models: Cell Lines Immortalized female Indian muntjac fibroblasts [29] Jerry W Shay Indian muntjac- H2B-GFP (female) In this paper N/A Indian muntjac- CENP-A-GFP (female) In this paper N/A Oligonucleotides 50-GCAGACATTGAGAGAATAA-30 (Ndc80) Sigma-Aldrich N/A 50-GCAGAATGGTTATACAAGT-30 (Mad2) Sigma-Aldrich N/A 50-GCTGCTGTTGCTGATGCTT-30 (CLASP1) Sigma-Aldrich N/A 50-GCGTCTCCACGTTTAAGAA-30 (Survivin) Sigma-Aldrich N/A 50-GAACCGTCAGCAAGACAA-30 (Kif4a) Sigma-Aldrich N/A 50-GCCAGGGACCTCATTTCAA-30 (Aurora A) Sigma-Aldrich N/A Recombinant DNA H2B-GFP Addgene plasmid # 11680 Geoff Wahl lab pSV-IRESneo3-CENP-A-EGFP [57] Patrick Meraldi Software and Algorithms Fiji/ImageJ ImageJ N/A CellProfiler [58] CellProfiler (http://cellprofiler.org) CellProfilerAnalyzer [58] CellProfiler (http://cellprofiler.org) MatLab8.1 The MathWorks https://www.mathworks.com/products/matlab.html Current Biology 28, 1344–1356.e1–e5, May 7, 2018 e1 ..
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