napi-iib (slc34a1; #vmps-6022)  (Biomol GmbH)

Journal: Poultry Science

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

doi: 10.1016/j.psj.2026.106762

Figure Lengend Snippet: Effects of dietary vitamin D 3 supplementation on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (19 d) (Experiment 1). The control and vitamin D 3 diets contained 0 and 1000 IU/kg vitamin D 3 , respectively. (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Techniques: Expressing, Control, Western Blot, Quantitative Proteomics

Journal: Poultry Science

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

doi: 10.1016/j.psj.2026.106762

Figure Lengend Snippet: Effects of the PKA inhibitor H-89 on jejunal NaPi-IIb mRNA and protein expression in broiler chickens (10–15 d) (Experiment 2). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6). NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot, Quantitative Proteomics

Journal: Poultry Science

Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens

doi: 10.1016/j.psj.2026.106762

Figure Lengend Snippet: Effects of the PKC inhibitor staurosporine on duodenal NaPi-IIb mRNA and protein expression in broiler chickens (9–13 d) (Experiment 3). (a) NaPi-IIb mRNA expression. (b, c) Representative immunoblots and quantitative analysis of NaPi-IIb protein expression. NaPi-IIb mRNA expression values are means ± SD of six replicates per treatment (1 broiler per replicate) (n = 6.) NaPi-IIb protein abundance values are means ± SD of four replicates per treatment (1 broiler per replicate) (n = 4). Values with different letters differ between treatments ( P < 0.05).

Article Snippet: PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China).

Techniques: Expressing, Western Blot, Quantitative Proteomics

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Genotype and gender from pups from 9 litters born from two independent heterozygous Slc34a2 breedings. ( B ) body weight, ( C ) food intake, ( D ) water intake, ( E ) fecal output and ( F ) urinary output of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Fecal Pi, ( B ) fecal Ca 2+ , ( C ) urinary Pi, ( D ) urinary Ca 2+ and ( E ) urinary creatinine of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05 and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques:

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: ( A ) Plasma Pi, ( B ) plasma Ca 2+ , ( C ) plasma intact FGF-23 and ( D ) plasma 1,25(OH) 2 vitamin D 3 of wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats fed standard chow (NPD) or challenged for 3 days with low Pi diet (LPD). Data are presented as means ± SEM. Statistical significance was calculated by ANOVA-Bonferroni. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Clinical Proteomics

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Expression of NaPi-IIb in total membranes isolated from mucosa of ( A ) duodenum and ( B ) jejunum from wild type (n = 4) and heterozygous (n = 5) Slc34a2 rats challenged for 3 days with low Pi diet. The abundance of the cotransporter was normalize to the expression of actin. The ratio in WT rats was considered as 100%.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Expressing, Isolation

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Intestines from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Lungs from ( A , C ) wild type and ( B , D ) Slc34a2 homozygous E18 embryos stained with ( A , B ) H&E or with ( C , D ) a NaPi-IIb antibody. Scale bars: 100 µm in ( A , B ); 50 µm in ( C , D ).

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Liver ( A , B ) and pancreas ( C , D ) from wild type ( A , C ) and Slc34a2 homozygous E18 embryos ( B , D ) stained with a NaPi-IIb antibody. Scale bars: 50 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining

Journal: Scientific Reports

Article Title: Constitutive depletion of Slc34a2 /NaPi-IIb in rats causes perinatal mortality

doi: 10.1038/s41598-021-86874-z

Figure Lengend Snippet: Kidneys from ( A ) wild type and ( B ) Slc34a2 homozygous E18 embryos stained with H&E. Scale bars: 250 µm.

Article Snippet: Rats with constitutive depletion of NaPi-IIb/ Slc34a2 were generated by Charles River using a Crispr-Cas approach.

Techniques: Staining