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pbabe nras 61k construct  (Addgene inc)


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    Structured Review

    Addgene inc pbabe nras 61k construct
    Pbabe Nras 61k Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+ras/pBabe+N-Ras+61K+(Plasmid+%2312543)/us10815484-577-7-16
    Average 92 stars, based on 18 article reviews
    pbabe nras 61k construct - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    other:

    Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA
    Article Snippet: pBabe NRAS 61K , Addgene , #12543.

    Amplification:

    Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid # 12543),( Khosravi-Far et al., 1996 )) subcloned into BglII and BamHI restriction enzyme sites. ..

    Article Title: Compositions and methods for treating cancer
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid #12543), (Khosravi-Far et al., Mol. ..

    Construct:

    Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid # 12543),( Khosravi-Far et al., 1996 )) subcloned into BglII and BamHI restriction enzyme sites. ..

    Article Title: Compositions and methods for treating cancer
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid #12543), (Khosravi-Far et al., Mol. ..

    Plasmid Preparation:

    Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid # 12543),( Khosravi-Far et al., 1996 )) subcloned into BglII and BamHI restriction enzyme sites. ..

    Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer.
    Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Channing Der (Addgene plasmid # 12543; RRID:Addgene_12543). ..

    Article Title: Compositions and methods for treating cancer
    Article Snippet: .. Full-length human NRAS 61K was amplified from pBabe NRAS 61K construct (a gift from Channing Der (Addgene plasmid #12543), (Khosravi-Far et al., Mol. ..

    Article Title: Genomic and pathological heterogeneity in clinically diagnosed small cell lung cancer in never/light smokers identifies therapeutically targetable alterations
    Article Snippet: .. pBabe NRAS Q61K plasmid was a gift from Channing Der (Addgene plasmid # 12543) (Khosravi‐Far et al. , ). pBabe NRAS Q61K plasmid or pBabe puro empty plasmid and a packaging plasmid pAmpho were co‐transfected into HEK293T cells. ..

    Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells.
    Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using pBabe N-Ras 61K (gift from Channing Der, Addgene plasmid # 12543) and pDONR223-MAPK14 (gift from William Hahn & David Root, Addgene plasmid # 23865) and ligated to pENTR/ D-TOPO TA (Invitrogen) using manufacturer's instructions (Hao et al., 2007; Johannessen et al., 2010). ..

    Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells
    Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation.

    Selection:

    Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer.
    Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Channing Der (Addgene plasmid # 12543; RRID:Addgene_12543). ..

    Expressing:

    Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer.
    Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Channing Der (Addgene plasmid # 12543; RRID:Addgene_12543). ..

    Clone Assay:

    Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells.
    Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using pBabe N-Ras 61K (gift from Channing Der, Addgene plasmid # 12543) and pDONR223-MAPK14 (gift from William Hahn & David Root, Addgene plasmid # 23865) and ligated to pENTR/ D-TOPO TA (Invitrogen) using manufacturer's instructions (Hao et al., 2007; Johannessen et al., 2010). ..

    Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells
    Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation.

    Polymerase Chain Reaction:

    Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells.
    Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using pBabe N-Ras 61K (gift from Channing Der, Addgene plasmid # 12543) and pDONR223-MAPK14 (gift from William Hahn & David Root, Addgene plasmid # 23865) and ligated to pENTR/ D-TOPO TA (Invitrogen) using manufacturer's instructions (Hao et al., 2007; Johannessen et al., 2010). ..

    Mutagenesis:

    Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells
    Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation.

    Sequencing:

    Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells
    Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation.



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    Image Search Results


    TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

    Journal: Aging Cell

    Article Title: Loss of p62 Binding Allows TIF ‐ IA Accumulation in Senescence, Which Promotes Phenotypic Changes to Nucleoli and the Senescence Associated Secretory Phenotype

    doi: 10.1111/acel.70334

    Figure Lengend Snippet: TIF‐IA protein accumulates in an animal model of OIS and in chronological and genetic models of ageing. (A) TIF‐IA and N‐ras immunohistochemistry were performed on liver sections from mice culled 6 days after hydrodynamic delivery of Nras G12V/D38A ( n = 5) or Nras G12V ( n = 5) transposons. Left: Representative images at 10× (scale bar 100 μm) and 40× (scale bar 20 μm) magnification. Right: QuPath software (v0.2.3) was used to quantify the percentage of TIF‐IA and N‐ras positive cells in five regions of interest (ROIs) per section. Pooled data (25 ROIs per condition) is shown. Mann–Whitney test determined significance. (B) Anti‐TIF‐IA immunohistochemistry was performed on colonic sections from young and old wild type (WT) and nfkb1 −/− mice ( n = 5 mice per condition). Left: Representative images at 10× and 40× magnification. Scale bar 20 μm. Right: QuPath software (v0.2.3) was used to quantify nuclear TIF‐IA intensity in five ROIs as above. Pooled data are shown. A Kruskal–Wallis test with Dunns multiple comparison was used to determine significance. ns, nonsignificant.

    Article Snippet: Sections were incubated overnight at 4°C with the primary antibodies: rabbit RRN3 (Abcam 251933) and mouse NRas (Santa Cruz Biotechnology sc‐31), diluted 1:500 in antibody diluent (Dako).

    Techniques: Animal Model, Immunohistochemistry, Software, MANN-WHITNEY, Comparison