pbabe nras 61k construct (Addgene inc)
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Pbabe Nras 61k Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+ras/pBabe+N-Ras+61K+(Plasmid+%2312543)/us10815484-577-7-16
Average 92 stars, based on 18 article reviews
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other:Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA Article Snippet: Amplification:Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA Article Snippet: .. Full-length human NRAS 61K was amplified from Article Title: Compositions and methods for treating cancer Article Snippet: .. Construct:Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA Article Snippet: .. Full-length human NRAS 61K was amplified from Article Title: Compositions and methods for treating cancer Article Snippet: .. Plasmid Preparation:Article Title: Oncogenic Role of THOR , a Conserved Cancer/Testis Long Noncoding RNA Article Snippet: .. Full-length human NRAS 61K was amplified from Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer. Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Article Title: Compositions and methods for treating cancer Article Snippet: .. Article Title: Genomic and pathological heterogeneity in clinically diagnosed small cell lung cancer in never/light smokers identifies therapeutically targetable alterations Article Snippet: .. pBabe NRAS Q61K plasmid was a gift from Channing Der (Addgene plasmid # 12543) ( Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells. Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation. Selection:Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer. Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Expressing:Article Title: Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer. Article Snippet: Twenty-four hour after infection, the growth medium was changed and 3 μg/ml (PC9) or 2 μg/ml (HCC827) puromycin (Sigma Aldrich, USA, Cat. No. p8833) was added for selection for 7 days. .. After selection, cells were analyzed for protein expression. pBABE-puro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764; RRID:Addgene_1764). pBabe-Puro-BRAFV600E was a gift from William Hahn (Addgene plasmid # 15269; RRID:Addgene_15269). pBabe-NRASQ61K was a gift from Clone Assay:Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells. Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation. Polymerase Chain Reaction:Article Title: NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells. Article Snippet: Gateway entry clone pENTR5-mitf was created by PCR amplifying full-length open reading frame using M24 Nac>Nac (Dorsky et al., 2000) (gift from Randall Moon, Addgene plasmid # 17174) and ligated to pENTR5-TOPO activated vector (Invitrogen) according to manufacturer's instructions (Khosravi-Far et al., 1996). .. Gateway middle entry clones pmiddle-NRASQ61K and pmiddleMAPK14 was created by PCR amplifying full-length open reading frame using Mutagenesis:Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation. Sequencing:Article Title: Constitutive Expression of NRAS with Q61R Driver Mutation Activates Processes of Epithelial–Mesenchymal Transition and Leads to Substantial Transcriptome Change of Nthy-ori 3–1 Thyroid Epithelial Cells Article Snippet: The Q61R mutation of the NRAS gene is one of the most frequent driver mutations of thyroid cancer.. Tumors with this mutation are characterized by invasion into blood vessels and formation of distant metastases.. To study the role of this mutation in the growth of thyroid cancer, we developed a model system on the basis of thyroid epithelial cell line Nthy ori 3 1 transduced by a lentiviral vector containing the NRAS gene with the Q61R mutation. |
