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mx3000 qpcr system  (Agilent technologies)


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    Structured Review

    Agilent technologies mx3000 qpcr system
    Mx3000 Qpcr System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mx3000/pmc11061197-252-11-14
    Average 90 stars, based on 1 article reviews
    mx3000 qpcr system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1B
    Article Snippet: .. Total RNA was extracted with Trizol (Roche Diagnostic GmbH, Germany) and retrotranscribed as previously described. cDNAs were quantified by quantitative real-time PCR on a MX3000 apparatus (Stratagene, La Jolla, CA, USA) by using specific primers. ..

    Article Title: A regulatory element associated to NAFLD in the promoter of DIO1 controls LDL-C, HDL-C and triglycerides in hepatic cells
    Article Snippet: Total RNA was isolated with RNeasy Mini kit (Qiagen) and quantified by Nanodrop spectrophotometer (ThermoScientific). .. Later cDNA synthesis was performed with SuperScript IV reverse trancriptase (Invitrogen) using random hexamers. qPCR reaction was performed with PowerUP Sybr Green Dye (Applied Biosystems) in a Mx3000 Pro (Stratagene). ..

    Article Title: Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells.
    Article Snippet: .. The reactions were performed in a MX3000 Multiplex Quantitative PCR system (Stratagene-Agilent) by using iTaq Universal SRYB Green 2X SuperMix kit (Bio-Rad) in duplicate in two independent experiments. ..

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    cDNA Synthesis:

    Article Title: A regulatory element associated to NAFLD in the promoter of DIO1 controls LDL-C, HDL-C and triglycerides in hepatic cells
    Article Snippet: Total RNA was isolated with RNeasy Mini kit (Qiagen) and quantified by Nanodrop spectrophotometer (ThermoScientific). .. Later cDNA synthesis was performed with SuperScript IV reverse trancriptase (Invitrogen) using random hexamers. qPCR reaction was performed with PowerUP Sybr Green Dye (Applied Biosystems) in a Mx3000 Pro (Stratagene). ..

    SYBR Green Assay:

    Article Title: A regulatory element associated to NAFLD in the promoter of DIO1 controls LDL-C, HDL-C and triglycerides in hepatic cells
    Article Snippet: Total RNA was isolated with RNeasy Mini kit (Qiagen) and quantified by Nanodrop spectrophotometer (ThermoScientific). .. Later cDNA synthesis was performed with SuperScript IV reverse trancriptase (Invitrogen) using random hexamers. qPCR reaction was performed with PowerUP Sybr Green Dye (Applied Biosystems) in a Mx3000 Pro (Stratagene). ..

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    other:

    Article Title: Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin
    Article Snippet: Amplifications were performed in an Agilent MX3000 real-time qPCR machine (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Redundancy of p75NTR neurotrophin receptor function in development, growth and fertility in the rat.
    Article Snippet: The reaction was performed on a Stratagene Mx3000 real-time PCR machine under the following conditions; 95 °C for 2 min followed by 40 cycles of 95 °C for 15 s, then 60 °C for 30 s, with a final, melting curve cycle consisting of 95 °C for 1 min, 60 °C for 30 s and 95 °C for 15 s. Western blot analysis The brains of E13.5 rats were lysed and 30 μg of protein combined with 5 μl 4 × LDS Sample Buffer (ThermoFisher, #NP0004) and 2 μl 10 × Sample Reducing Agent (ThermoFisher, #NP0007) in a final volume of 20 μl with water.

    Article Title: Inhibitor of inflammatory conditions
    Article Snippet: Real-time PCR was carried out using an Mx3000® QPCR System (Stratagene).

    Multiplex Assay:

    Article Title: Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells.
    Article Snippet: .. The reactions were performed in a MX3000 Multiplex Quantitative PCR system (Stratagene-Agilent) by using iTaq Universal SRYB Green 2X SuperMix kit (Bio-Rad) in duplicate in two independent experiments. ..

    Stripping Membranes:

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..

    Quantitative RT-PCR:

    Article Title: Differential expression and global analysis of miR156/ SQUAMOSA promoter binding-like proteins (SPL) module in oat
    Article Snippet: .. Optical strip tubes were used to perform qRT-PCR analysis using the Mx3000 qPCR system (Stratagene, USA) using 1 μl diluted cDNA, 10 μM gene-specific primers, and 10 μl Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent, USA). ..



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