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mutant dna sequences of chlopsid fp  (GenScript corporation)

 
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    GenScript corporation mutant dna sequences of chlopsid fp
    Mutant Dna Sequences Of Chlopsid Fp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+dna+sequence/mutant+dna+sequences+of+chlopsid+fp/pm32096074-40-4-9
    Average 90 stars, based on 1 article reviews
    mutant dna sequences of chlopsid fp - by Bioz Stars, 2026-11
    90/100 stars

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    Mutagenesis:

    Article Title: Disrupting Fluorescence by Mutagenesis in a Green Fluorescent Fatty Acid Binding Protein from a Marine Eel.
    Article Snippet: Biofluorescence has been found to be an increasingly widespread phenomenon in the ocean.. The reclusive Caribbean chlopsid eel, Kaupichthys hyoproroides displays bright green fluorescence in its native marine environment.. We have previously shown the fluorescence to be attributed to a fluorescent fatty acid-binding protein, Chlopsid FP, part of a larger family of fluorescent fatty acid-binding proteins, including the homologous UnaG.



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    Image Search Results


    Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Journal: Developmental cell

    Article Title: Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics.

    doi: 10.1016/j.devcel.2025.02.008

    Figure Lengend Snippet: Figure 1. Disruption of apical-basal polarity by dominant-negative CDH1 mutants in the Matrigel overlay culture system or by Cdc42 knockout in the developing mouse pancreas alters beta cell specification (A) Design of dominant-negative CDH1 mutants with deletions in the extracellular domain (CDH1DE) or the p120ctn-binding site (CDH1DP). mCh, internal ribosome entry site (IRES)-coupled mCh (red fluorescent protein) reporter. (B) Confocal images of S5 (day 13) differentiated cells (± 72 h Dox treatment). EZR, EZRIN, green; BCAT, beta-catenin, gray; mCh, mCherry, red. Scale bar, 50 mm. (C) Flow cytometry quantification of INS single-positive beta cells and GCG positive alpha cells in mCherry+ and mCherry subpopulations after Dox treatment (S4–S6). CDH1DE and CDH1DP mutations led to the same degree of reduction in the INS+GCG/GCG+ ratio in mCherry+ mutant cells compared with mCherry

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Latrunculin B Sigma Aldrich #L5288 Heparin Sigma Aldrich #H3149-100KU Bovine serum albumin (BSA) Proliant #7500804 Bovine serum albumin (BSA) Roche #10775835001 Bovine serum albumin (BSA) Sigma Aldrich #B4287 Y-27632 Merck Millipore #688000 45 % glucose solution in water Sigma Aldrich #G8769 7.5% sodium bicarbonate solution Thermo Fisher #25080094 Forskolin Sigma Aldrich #F6886 3-Isobutyl-1-methylxanthin Thermo Fisher #J64598.MC G418 Thermo Fisher #10131027 Hygromycin Thermo Fisher #10687010 Doxycycline Sigma-Aldrich #D3447 Critical commercial assays RNeasy Mini Kit Qiagen #74106 RNeasy Micro Kit Qiagen #74004 iScript cDNA Synthesis Kit BIO-RAD #1708891 KAPA HiFi HotStart Library Amplification Kit Roche #07958951001 cAMP XP@ Assay Kit Cell Signaling #4339 P3 Primary Cell Kit Lonza #V4XP-3024 LIVE/DEAD Fixable Violet Dead Cell Stain Kit Thermo Fisher #L34964 Deposited data scRNA-seq datasets This paper GSE224286 Experimental models: Cell lines SA121 Takara bio N/A H1 Beydag-Tasöz et al.34 (University of Copenhagen) N/A Experimental models: Organisms/strains Cdc42fl/fl R26-YFP+/+ mice In house N/A Pdx1-Cre+/- Cdc42fl/wt mice In house N/A Oligonucleotides siRNA against EGR1 Thermo Fisher #S4537; #S4538 Negative control siRNA Thermo Fisher #4390843 Recombinant DNA Mutant CDH1 sequences Addgene #45770; #45773 PiggyBac destination vector PB-TAC-ERN Addgene #80475 Cas9 expression vector (pX458-HF1) Addgene #48138 Software and algorithms Adobe Photoshop 2022 Adobe https://www.adobe.com Adobe Illustrator 2023 Adobe https://www.adobe.com Imaris 8.4, 9.0 Oxford Instruments https://imaris.oxinst.com/ Fiji 2.0/ImageJ NIH Image http://imagej.nih.gov/ij GraphPad Prism 10 GraphPad https://www.graphpad.com FlowJo 10 BD https://www.flowjo.com FCS Express 7 De Novo Software https://denovosoftware.com ZEN (blue edition) ZEISS https://www.micro-shop.zeiss.com Developmental Cell 60, 1–13.e1–e5, July 7, 2025 e2

    Techniques: Disruption, Dominant Negative Mutation, Knock-Out, Binding Assay, Flow Cytometry, Mutagenesis