multiscribe reverse transcriptase (Promega)
90
Structured Review
Promega
multiscribe reverse transcriptase
Multiscribe Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+reverse+transcriptase+using+random+hexamers/multiscribe+reverse+transcriptase/pmc02875325-78-55-58
Average 90 stars, based on 1 article reviews
Multiscribe Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+reverse+transcriptase+using+random+hexamers/multiscribe+reverse+transcriptase/pmc02875325-78-55-58
Average 90 stars, based on 1 article reviews
multiscribe reverse transcriptase - by Bioz Stars,
2026-09
90/100 stars
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Reverse Transcription:Article Title: Evolutionary trade-offs constraining the MHC gene expansion: beyond simple TCR depletion model Article Snippet: .. Next, the temperature was lowered to 42°C, and mix 2 was added to each sample (5× First-strand buffer, 1μl 20mM DTT, 2.5μl of 10μM SmartNNNNa, 3μl of 10mM dNTP mix, 1.5μl of Article Title: Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221. Article Snippet: Ethnopharmacological relevance: In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu’s functions including Kidney deficiency.. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection.. Aim of the study: To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by high dietary fructose feeding, a major contributor for insulin resistance in glomerular podocyte dysfunction. Article Title: Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation. Article Snippet: Following homogenization of the tissue samples, total RNA was extracted from the ipsilateral lumbar spinal cord using an RNA Isolation kit (Invitrogen; Thermo Fisher Scientific, Inc.) and RNA was detected using a spectrophotometer (Epoch 2; BioTek Instruments, Inc., Winooski, VT, USA). cDNA was synthesized by reverse transcription (RT). .. The RT reaction was carried out in a 20 μl total reaction volume, containing 4 μl 5X RT buffer, 4 μl 2.5 mM dNTPs, 1 μl Article Title: Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-κB and MAPK/caspase signaling pathways Article Snippet: .. The RT reaction contained RNA samples including 2 μ g purified total RNA, 2 μ l 10 μ M oligo(dT) primers, 5 μ l 5xRT buffer (Promega Corporation, Madison, WI, USA), 1 μ l 40 U/ μ l Article Title: Combined macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose-derived mesenchymal stem cells Article Snippet: The mixture was incubated at 37 °C for 25 min then at 75 °C for 5 min in a thermal cycler (Bio-Rad, Germany). .. RNA samples were reverse transcribed into cDNA in a master mix containing 45 μL RT-Mix, 3 μL RNase inhibitor, and 3 μL Article Title: Polydatin prevents fructose-induced liver inflammation and lipid deposition through increasing miR-200a to regulate Keap1/Nrf2 pathway Article Snippet: Reverse Transcription System Kit, dNTPs and RNase inhibitor were obtained from Vazyme Biotechnology Co., Ltd (Nanjing, China), respectively. iTaqTM Universal SYBR ® Green Supermix was got from Bio-Rad Inc. (California, USA). .. Article Title: Retinoic acid induced 16 deficiency exacerbates high-fat diet-induced steatohepatitis in mice. Article Snippet: Funding information National Key R&D Program of China., Grant/ Award Number: 2018YFC1200603 Non-alcoholic fatty liver disease (NAFLD) associated with obesity may progress to non-alcoholic steatohepatitis, cirrhosis and hepatocellular carcinoma (HCC).. Retinoic acid induced 16 (RAI16) plays an important role in cell apoptosis and is also a potential marker for HCC.. Here we aimed to test the effect of RAI16 deficiency on liver pathology in high-fat diet (HFD) fed mice. Article Title: Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1. Article Snippet: High fructose intake is a risk of glomerular podocyte dysfunction.. Podocyte apoptosis has emerged as a major cause of podocyte loss, exacerbating proteinuria.. Magnesium isoglycyrrhizinate (MgIG) is usually used as a hepatoprotective agent in clinic. Reporter Assay:Article Title: Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221. Article Snippet: Ethnopharmacological relevance: In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu’s functions including Kidney deficiency.. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection.. Aim of the study: To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by high dietary fructose feeding, a major contributor for insulin resistance in glomerular podocyte dysfunction. Article Title: Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1. Article Snippet: High fructose intake is a risk of glomerular podocyte dysfunction.. Podocyte apoptosis has emerged as a major cause of podocyte loss, exacerbating proteinuria.. Magnesium isoglycyrrhizinate (MgIG) is usually used as a hepatoprotective agent in clinic. Purification:Article Title: Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-κB and MAPK/caspase signaling pathways Article Snippet: .. The RT reaction contained RNA samples including 2 μ g purified total RNA, 2 μ l 10 μ M oligo(dT) primers, 5 μ l 5xRT buffer (Promega Corporation, Madison, WI, USA), 1 μ l 40 U/ μ l Real-time Polymerase Chain Reaction:Article Title: Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-κB and MAPK/caspase signaling pathways Article Snippet: .. The RT reaction contained RNA samples including 2 μ g purified total RNA, 2 μ l 10 μ M oligo(dT) primers, 5 μ l 5xRT buffer (Promega Corporation, Madison, WI, USA), 1 μ l 40 U/ μ l Isolation:Article Title: Retinoic acid induced 16 deficiency exacerbates high-fat diet-induced steatohepatitis in mice. Article Snippet: Funding information National Key R&D Program of China., Grant/ Award Number: 2018YFC1200603 Non-alcoholic fatty liver disease (NAFLD) associated with obesity may progress to non-alcoholic steatohepatitis, cirrhosis and hepatocellular carcinoma (HCC).. Retinoic acid induced 16 (RAI16) plays an important role in cell apoptosis and is also a potential marker for HCC.. Here we aimed to test the effect of RAI16 deficiency on liver pathology in high-fat diet (HFD) fed mice. SYBR Green Assay:Article Title: Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1. Article Snippet: High fructose intake is a risk of glomerular podocyte dysfunction.. Podocyte apoptosis has emerged as a major cause of podocyte loss, exacerbating proteinuria.. Magnesium isoglycyrrhizinate (MgIG) is usually used as a hepatoprotective agent in clinic. |