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lunascript multiplex 1 step rt pcr kit  (New England Biolabs)


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    Structured Review

    New England Biolabs lunascript multiplex 1 step rt pcr kit
    Lunascript Multiplex 1 Step Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+kit/LunaScript+Multiplex+One-Step+RT-PCR+Kit/pm39721564-67-23-28
    Average 94 stars, based on 26 article reviews
    lunascript multiplex 1 step rt pcr kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S), NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and NEB LunaScript RT Master Mix Kit Primer-free (Cat no. E3025L) with BVDv specific primers) were tested using manufacturer’s instructions with two BVDv positive clinical samples. ..

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit [cat. no. E1555S], NEB LunaScript RT SuperMix Kit [cat. no. E3010L], and NEB LunaScript RT Master Mix Kit Primer-free [cat. no. E3025L] with BVDv-specific primers) were tested using manufactur er’s instructions with two BVDv-positive clinical samples. ..

    Multiplex Assay:

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S), NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and NEB LunaScript RT Master Mix Kit Primer-free (Cat no. E3025L) with BVDv specific primers) were tested using manufacturer’s instructions with two BVDv positive clinical samples. ..

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit [cat. no. E1555S], NEB LunaScript RT SuperMix Kit [cat. no. E3010L], and NEB LunaScript RT Master Mix Kit Primer-free [cat. no. E3025L] with BVDv-specific primers) were tested using manufactur er’s instructions with two BVDv-positive clinical samples. ..

    Article Title: The molecular epidemiology of respiratory syncytial virus in Ontario, Canada from 2022-2024 using a custom whole genome sequencing assay and analytics package.
    Article Snippet: .. Viral RNA (5 μL) and 50 pmol of primers were combined in two 25 μL RT-PCR reactions per sample (Pool_1 and Pool_2) using LunaScript Multiplex 1-Step RT-PCR kit (New England Biolab, Ipswich, MA). ..

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, Cat no. E3025L) with BVDv -specific primers were sequenced.

    Article Title: Improved influenza A whole-genome sequencing protocol
    Article Snippet: .. RT-PCR Kit , SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) , SuperScript IV One-Step RT-PCR System (Invitrogen) , LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. One-step RT-PCR , Yes , Yes , Yes.

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (cat. no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (cat. no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, cat. no. E3025L) with BVDv-specific primers were sequenced.

    One Step RT-PCR:

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S), NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and NEB LunaScript RT Master Mix Kit Primer-free (Cat no. E3025L) with BVDv specific primers) were tested using manufacturer’s instructions with two BVDv positive clinical samples. ..

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Three different reverse transcriptase kits (NEB LunaScript Multiplex One-Step RT-PCR Kit [cat. no. E1555S], NEB LunaScript RT SuperMix Kit [cat. no. E3010L], and NEB LunaScript RT Master Mix Kit Primer-free [cat. no. E3025L] with BVDv-specific primers) were tested using manufactur er’s instructions with two BVDv-positive clinical samples. ..

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, Cat no. E3025L) with BVDv -specific primers were sequenced.

    Article Title: Improved influenza A whole-genome sequencing protocol
    Article Snippet: .. RT-PCR Kit , SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) , SuperScript IV One-Step RT-PCR System (Invitrogen) , LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. One-step RT-PCR , Yes , Yes , Yes.

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (cat. no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (cat. no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, cat. no. E3025L) with BVDv-specific primers were sequenced.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The molecular epidemiology of respiratory syncytial virus in Ontario, Canada from 2022-2024 using a custom whole genome sequencing assay and analytics package.
    Article Snippet: .. Viral RNA (5 μL) and 50 pmol of primers were combined in two 25 μL RT-PCR reactions per sample (Pool_1 and Pool_2) using LunaScript Multiplex 1-Step RT-PCR kit (New England Biolab, Ipswich, MA). ..

    Article Title: Improved influenza A whole-genome sequencing protocol
    Article Snippet: .. RT-PCR Kit , SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) , SuperScript IV One-Step RT-PCR System (Invitrogen) , LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs). .. One-step RT-PCR , Yes , Yes , Yes.

    Nucleic Acid Electrophoresis:

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, Cat no. E3025L) with BVDv -specific primers were sequenced.

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (cat. no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (cat. no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, cat. no. E3025L) with BVDv-specific primers were sequenced.

    Polymerase Chain Reaction:

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (Cat no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (Cat no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, Cat no. E3025L) with BVDv -specific primers were sequenced.

    Article Title: Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure.
    Article Snippet: .. Gel electrophoresis results from the NEB LunaScript Multiplex One-Step RT-PCR Kit (cat. no. E1555S) indicated a significant amount of short non-specific amplifications, so the PCR product from this kit was not sequenced. .. PCR products from the NEB LunaScript RT SuperMix Kit (cat. no. E3010L) and the NEB LunaScript RT Master Mix Kit (Primer-free, cat. no. E3025L) with BVDv-specific primers were sequenced.



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