Review




Structured Review

JenLab GmbH custom built 5d multiphoton flim microscope
MPM and <t>FLIM</t> analysis of DCF-exposed HDFs. ( A – D ) FLIM colour-coded imaging of: untreated HDFs ( A ); DCF-treated HDFs after 6 h incubation time ( B ); DCF-treated HDFs assessed as apoptotic ( C ) and necrotic ( D ) after 24 h incubation time. Scale bar = 20 µm. ( E ) Histograms of α 1 % distribution after FLIM analysis. ( F ) Average nucleus diameter.
Custom Built 5d Multiphoton Flim Microscope, supplied by JenLab GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphoton+microscope/custom+built+5d+multiphoton+flim+microscope/pmc06401126-185-5-11
Average 90 stars, based on 1 article reviews
custom built 5d multiphoton flim microscope - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Controlled and tuneable drug release from electrospun fibers and a non-invasive approach for cytotoxicity testing"

Article Title: Controlled and tuneable drug release from electrospun fibers and a non-invasive approach for cytotoxicity testing

Journal: Scientific Reports

doi: 10.1038/s41598-019-40079-7

MPM and FLIM analysis of DCF-exposed HDFs. ( A – D ) FLIM colour-coded imaging of: untreated HDFs ( A ); DCF-treated HDFs after 6 h incubation time ( B ); DCF-treated HDFs assessed as apoptotic ( C ) and necrotic ( D ) after 24 h incubation time. Scale bar = 20 µm. ( E ) Histograms of α 1 % distribution after FLIM analysis. ( F ) Average nucleus diameter.
Figure Legend Snippet: MPM and FLIM analysis of DCF-exposed HDFs. ( A – D ) FLIM colour-coded imaging of: untreated HDFs ( A ); DCF-treated HDFs after 6 h incubation time ( B ); DCF-treated HDFs assessed as apoptotic ( C ) and necrotic ( D ) after 24 h incubation time. Scale bar = 20 µm. ( E ) Histograms of α 1 % distribution after FLIM analysis. ( F ) Average nucleus diameter.

Techniques Used: Imaging, Incubation

Related Articles

Fluorescence:

Article Title: Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models
Article Snippet: To elicit the effect of EGF on the cultures, recombinant human EGF (R&D Systems Inc., Abingdon, UK) was added at a final concentration of 10 ng/ml in culture medium from day 0 onwards. .. Fluorescence lifetime images of 3D Caco-2 models were acquired with a custom built 5D multiphoton FLIM microscope (JenLab GmbH, Jena, Germany). .. Two-photon excitation was generated using a Ti: Sapphire femtosecond laser (MaiTai XF1 Spectra Physics, United States, Santa Clara).

Microscopy:

Article Title: Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models
Article Snippet: To elicit the effect of EGF on the cultures, recombinant human EGF (R&D Systems Inc., Abingdon, UK) was added at a final concentration of 10 ng/ml in culture medium from day 0 onwards. .. Fluorescence lifetime images of 3D Caco-2 models were acquired with a custom built 5D multiphoton FLIM microscope (JenLab GmbH, Jena, Germany). .. Two-photon excitation was generated using a Ti: Sapphire femtosecond laser (MaiTai XF1 Spectra Physics, United States, Santa Clara).

Article Title: Controlled and tuneable drug release from electrospun fibers and a non-invasive approach for cytotoxicity testing
Article Snippet: Cell culture medium was changed every 3 days and cells were passaged or seeded using trypsin-EDTA (15090046, PAA Laboratories) at approximately 70% confluence. .. Images were acquired with a custom built 5D multiphoton FLIM microscope (JenLab GmbH, Jena, Germany). .. Two-photon excitation was generated using a Ti: Sapphire femtosecond laser (MaiTai XF1 Spectra Physics, United States, Santa Clara).



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Image Search Results


Protective effects of hiPSC-EV treatment on structural integrity and viability of hAO during IRI. (A) Experimental timeline. (B-E) Representative bright-field images (4x magnifications, EVOS microscope) of hAO pre-ischemia, post-ischemia (16 hours) and different reperfusion phases, show onset of cellular degeneration (black arrows). The bottom row of each image highlights morphological damage. (F) Label-free imaging of hAO pre-ischemia and different reperfusion phases (6 and 24 hours) performed using multiphoton microscopy. (G) hAO viability assessed by cellular ATP-dependent luminescence (RLU) at each time point. Data are mean ± SD (n = 3). Samples without hiPSC-EV treatment: w/o hiPSC-EV. Samples with hiPSC-EV treatment: with hiPSC-EV. *p < 0.05 versus pre-ischemia (control); # p < 0.05 versus IRI without hiPSC-EV.

Journal: International Journal of Biological Sciences

Article Title: Extracellular Vesicles from Human Induced Pluripotent Stem Cells Reduce Lung Ischemia-Reperfusion Injury via Cytoprotective Mechanisms in both 2D Cultures and a Human Airway Organoid Model

doi: 10.7150/ijbs.134686

Figure Lengend Snippet: Protective effects of hiPSC-EV treatment on structural integrity and viability of hAO during IRI. (A) Experimental timeline. (B-E) Representative bright-field images (4x magnifications, EVOS microscope) of hAO pre-ischemia, post-ischemia (16 hours) and different reperfusion phases, show onset of cellular degeneration (black arrows). The bottom row of each image highlights morphological damage. (F) Label-free imaging of hAO pre-ischemia and different reperfusion phases (6 and 24 hours) performed using multiphoton microscopy. (G) hAO viability assessed by cellular ATP-dependent luminescence (RLU) at each time point. Data are mean ± SD (n = 3). Samples without hiPSC-EV treatment: w/o hiPSC-EV. Samples with hiPSC-EV treatment: with hiPSC-EV. *p < 0.05 versus pre-ischemia (control); # p < 0.05 versus IRI without hiPSC-EV.

Article Snippet: Label-free imaging of hAO was also performed using a multiphoton microscopy Leica Stellaris DIVE 8 (Leica Microsystems, Germany), enabling visualization of cellular structures and intrinsic properties while maintaining the native organoid environment.

Techniques: Microscopy, Label-free Imaging, Control