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multiphoton microscope thorlabs bergamo ii star  (Thorlabs)

 
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    Structured Review

    Thorlabs multiphoton microscope thorlabs bergamo ii star
    Multiphoton Microscope Thorlabs Bergamo Ii Star, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiphoton+microscope/microscope+photon+two/pm42101939-54-0-2
    Average 86 stars, based on 1 article reviews
    multiphoton microscope thorlabs bergamo ii star - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Protocol for a saline-free surgical preparation of adult Drosophila for chronic in vivo brain imaging.
    Article Snippet: Metal bars Thorlabs ER90C, ER1.5 Flat washer McMaster-Carr Cat#93475A195 Kimwipes Kimberly-Clark Cat#34120 Distilled water Thermo Fisher Scientific Cat#15230162 Sucrose Sigma-Aldrich Cat#S5016-500G Tungsten needle (1 μm tip) Fine Science Tools Cat#10130-05 Moria nickel-plated pin holder Fine Science Tools Cat#26016-12 Minutien stainless-steel pins Fine Science Tools Cat#26002-20 Nickel-plated pin holder Fine Science Tools Cat#26018-17 UV-curable glue for mounting Norland Products NOA 81 UV-curable glue for cuticle sealing Norland Products NOA 68 Opticure UV/VIS wand Norland Products Cat#5000 #0 glass coverslips Electron Microscopy Sciences Cat#72198-10 Cell culture dishes with 4 inner rings Cellstar Cat#627170 Desktop humidifier Pure Enrichment PEHUMINI-2 UV safety glasses Thorlabs LG3 Stereoscope with light source Thomas Scientific Ivesta 3 Water-immersion objective Olympus XLUMPLFLN High-power LED light source Prizmatix 475-SR sCMOS camera Hamamatsu Fusion-BT Fixed wavelength femtosecond laser Coherent Axon-920-1 TPC 474/27 nm excitation filter Semrock FL-004596 488-nm dichroic mirror Semrock FL-007135 520/35-nm emission filter Semrock FL-004484 Epifluorescence microscope Custom-built N.A. .. Multiphoton microscope Thorlabs Bergamo II STAR Protocols 7, 104550, June 19, 2026 3 ..

    Article Title: Magnetogenetic control of endogenous calcium signaling via ROS-sensitive ion channels reveals astrocytic regulation of neural circuit activity
    Article Snippet: .. In vivo 2PFM was performed using a commercial multiphoton microscope (Bergamo® II, Thorlabs). .. Experiments were conducted using an upright AxioExaminer Z.1 microscope (Zeiss) equipped with an Axiocam 702 mono camera controlled by Zen 2.6 software.

    Article Title: Protocol for a saline-free surgical preparation of adult Drosophila for chronic in vivo brain imaging.
    Article Snippet: We acquired images at 1002 Hz for voltage imaging, using a scientific grade sCMOS camera (Fusion BT, Hamamatsu) and 2 3 2 pixel binning. .. Note: For the calcium imaging demonstration, we used a Thorlabs Bergamo II two-photon microscope for calcium imaging with a 1.0 NA water-immersion 203 XLUMPlanFLN objective lens (N20X-PFH, Olympus) and a 920 nm fixed wavelength laser (Axon-920-1 TPC, Coherent). ..

    Article Title: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities
    Article Snippet: A 488 nm light (laser diode, 480 μW at the patch cord tip – 3.8 mW mm −2 ) was alternated (10 × 10 s ON/10 s OFF) after 2 min from the start of the PinkyCaMP photometry recording in the OF. After optogenetic silencing, mice explored the OF arena for a further 2 min. A tailored MATLAB code was used to extract, process and analyze PinkyCaMP signals. .. Two-photon imaging was performed using an upright Thorlabs Bergamo II galvo-resonant scanning microscope, equipped with a Ti:sapphire excitation laser (Chameleon Ultra II, Coherent) and a ×10 0.5 NA objective (TL10X-2P, Thorlabs). ..

    Article Title: Non-uniform endogenous regeneration of olfactory sensory neuron axons across the mouse olfactory bulb.
    Article Snippet: .. Chronic in vivo 2-photon imaging was performed using a Bergamo II 2-photon microscope (ThorLabs) equipped with a SemiApo 20×/1.0NA water-immersion objective (Olympus) and an Insight X3 IR laser (Newport) mode-locked at 935 nm. ..

    Article Title: Embryonic dopaminergic neuron activity sustains lifelong locomotion in Drosophila.
    Article Snippet: .. In vivo functional calcium imaging was performed using a Thorlabs Bergamo II two-photon microscope with Coherent Axon laser. .. The jGCaMP8s indicator was excited with 920 nm light from the Axon 920 TPC laser (Coherent Inc).

    Article Title: Translatome profiling reveals opposing alterations in inhibitory and excitatory neurons of fragile X mice and identifies EPAC2 as a therapeutic target.
    Article Snippet: .. We used a commercial 2-photon (2P) microscope (DIY Bergamo, ThorLabs) equipped with galvo-resonant scanning mirrors, amplified non-cooled GaAsP photomultiplier tubes (Hamamatsu), a 16X objective (0.8NA, Olympus, Thor N16XLWD-PF), and ThorImage software. .. The microscope was coupled to a Chameleon Ultra II Ti:sapphire laser (Coherent) tuned to 930 nm, and the average power at the sample was kept <80 mW.

    In Vivo:

    Article Title: Magnetogenetic control of endogenous calcium signaling via ROS-sensitive ion channels reveals astrocytic regulation of neural circuit activity
    Article Snippet: .. In vivo 2PFM was performed using a commercial multiphoton microscope (Bergamo® II, Thorlabs). .. Experiments were conducted using an upright AxioExaminer Z.1 microscope (Zeiss) equipped with an Axiocam 702 mono camera controlled by Zen 2.6 software.

    Article Title: Non-uniform endogenous regeneration of olfactory sensory neuron axons across the mouse olfactory bulb.
    Article Snippet: .. Chronic in vivo 2-photon imaging was performed using a Bergamo II 2-photon microscope (ThorLabs) equipped with a SemiApo 20×/1.0NA water-immersion objective (Olympus) and an Insight X3 IR laser (Newport) mode-locked at 935 nm. ..

    Article Title: Embryonic dopaminergic neuron activity sustains lifelong locomotion in Drosophila.
    Article Snippet: .. In vivo functional calcium imaging was performed using a Thorlabs Bergamo II two-photon microscope with Coherent Axon laser. .. The jGCaMP8s indicator was excited with 920 nm light from the Axon 920 TPC laser (Coherent Inc).

    Imaging:

    Article Title: Pupil-linked arousal heterogeneously modulates cell-type-specific sensory processing.
    Article Snippet: .. All two- photon imaging (Bergamo, Thorlabs) was of the right ACtx. ..

    Article Title: Protocol for a saline-free surgical preparation of adult Drosophila for chronic in vivo brain imaging.
    Article Snippet: We acquired images at 1002 Hz for voltage imaging, using a scientific grade sCMOS camera (Fusion BT, Hamamatsu) and 2 3 2 pixel binning. .. Note: For the calcium imaging demonstration, we used a Thorlabs Bergamo II two-photon microscope for calcium imaging with a 1.0 NA water-immersion 203 XLUMPlanFLN objective lens (N20X-PFH, Olympus) and a 920 nm fixed wavelength laser (Axon-920-1 TPC, Coherent). ..

    Article Title: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities
    Article Snippet: A 488 nm light (laser diode, 480 μW at the patch cord tip – 3.8 mW mm −2 ) was alternated (10 × 10 s ON/10 s OFF) after 2 min from the start of the PinkyCaMP photometry recording in the OF. After optogenetic silencing, mice explored the OF arena for a further 2 min. A tailored MATLAB code was used to extract, process and analyze PinkyCaMP signals. .. Two-photon imaging was performed using an upright Thorlabs Bergamo II galvo-resonant scanning microscope, equipped with a Ti:sapphire excitation laser (Chameleon Ultra II, Coherent) and a ×10 0.5 NA objective (TL10X-2P, Thorlabs). ..

    Article Title: Non-uniform endogenous regeneration of olfactory sensory neuron axons across the mouse olfactory bulb.
    Article Snippet: .. Chronic in vivo 2-photon imaging was performed using a Bergamo II 2-photon microscope (ThorLabs) equipped with a SemiApo 20×/1.0NA water-immersion objective (Olympus) and an Insight X3 IR laser (Newport) mode-locked at 935 nm. ..

    Article Title: Embryonic dopaminergic neuron activity sustains lifelong locomotion in Drosophila.
    Article Snippet: .. In vivo functional calcium imaging was performed using a Thorlabs Bergamo II two-photon microscope with Coherent Axon laser. .. The jGCaMP8s indicator was excited with 920 nm light from the Axon 920 TPC laser (Coherent Inc).

    Functional Assay:

    Article Title: Embryonic dopaminergic neuron activity sustains lifelong locomotion in Drosophila.
    Article Snippet: .. In vivo functional calcium imaging was performed using a Thorlabs Bergamo II two-photon microscope with Coherent Axon laser. .. The jGCaMP8s indicator was excited with 920 nm light from the Axon 920 TPC laser (Coherent Inc).

    Amplification:

    Article Title: Translatome profiling reveals opposing alterations in inhibitory and excitatory neurons of fragile X mice and identifies EPAC2 as a therapeutic target.
    Article Snippet: .. We used a commercial 2-photon (2P) microscope (DIY Bergamo, ThorLabs) equipped with galvo-resonant scanning mirrors, amplified non-cooled GaAsP photomultiplier tubes (Hamamatsu), a 16X objective (0.8NA, Olympus, Thor N16XLWD-PF), and ThorImage software. .. The microscope was coupled to a Chameleon Ultra II Ti:sapphire laser (Coherent) tuned to 930 nm, and the average power at the sample was kept <80 mW.

    Software:

    Article Title: Translatome profiling reveals opposing alterations in inhibitory and excitatory neurons of fragile X mice and identifies EPAC2 as a therapeutic target.
    Article Snippet: .. We used a commercial 2-photon (2P) microscope (DIY Bergamo, ThorLabs) equipped with galvo-resonant scanning mirrors, amplified non-cooled GaAsP photomultiplier tubes (Hamamatsu), a 16X objective (0.8NA, Olympus, Thor N16XLWD-PF), and ThorImage software. .. The microscope was coupled to a Chameleon Ultra II Ti:sapphire laser (Coherent) tuned to 930 nm, and the average power at the sample was kept <80 mW.



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