multiphor ii electrophoresis unit (Amersham Life Sciences Inc)
90
Structured Review
Amersham Life Sciences Inc
multiphor ii electrophoresis unit
Multiphor Ii Electrophoresis Unit, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+unit/multiphor+ii+electrophoresis+unit/pmc06123647-962-30-40
Average 90 stars, based on 1 article reviews
Multiphor Ii Electrophoresis Unit, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+unit/multiphor+ii+electrophoresis+unit/pmc06123647-962-30-40
Average 90 stars, based on 1 article reviews
multiphor ii electrophoresis unit - by Bioz Stars,
2026-09
90/100 stars
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Electrofocusing:Article Title: High-fat diet-induced changes in liver thioredoxin and thioredoxin reductase as a novel feature of insulin resistance Article Snippet: .. Isoelectric focusing (IEF) was conducted on pH 3–10 or pH 5–8 17-cm immobilized pH gradient (IPG) strips, respectively, in Article Title: 70-kDa Heat Shock Protein Downregulates Dynamin in Experimental Stroke Article Snippet: 2-D PAGE: IPG dry strips were equilibrated for 12-16 hours with 7 M urea, thiourea containing 2% 3-[(3- Cholamidopropyl)dimethylammonio]-1-prpanesulfonate (CHAPS, Sigma), 1% dithiothreitol (DTT, Sigma), 1% pharmalyte in 200 μg samples. .. Isoelectric focusing (IEF) was performed at 20°C using a Article Title: Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil. Article Snippet: FK506 (tacrolimus) is a 23-membered polyketide macrolide that possesses powerful immunosuppressant activity.. In this study, feeding soybean oil into the fermentation culture of Streptomyces tsukubaensis improved FK506 production by 88.8%.. To decipher the overproduction mechanism, comparative proteomic and metabolomic analysis was carried out. Article Title: SPATC 1L maintains the integrity of the sperm head‐tail junction Article Snippet: .. For the first dimension of 2DE, a sample of the soluble fraction containing 200 μg protein was loaded onto the gel, followed by isoelectric focusing (IEF) at 20°C using a Article Title: Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis Article Snippet: For the first dimension, electrophoresis was applied onto IPG strips of 18 cm, pH=4–7 (Bio-Rad, Hercules, CA) rehydrated by loading the samples diluted with rehydration buffer (8M urea, 4% CHAPS, 2% ampholyte, 50 mM DTT, and traces of bromophenol blue) overnight. .. After the gel rehydration, IEF was performed at 300V/ 1h, 500V/ 1h, 1000V/ 2h, and 3500V/ 12h using a Electrophoresis:Article Title: High-fat diet-induced changes in liver thioredoxin and thioredoxin reductase as a novel feature of insulin resistance Article Snippet: .. Isoelectric focusing (IEF) was conducted on pH 3–10 or pH 5–8 17-cm immobilized pH gradient (IPG) strips, respectively, in Article Title: 70-kDa Heat Shock Protein Downregulates Dynamin in Experimental Stroke Article Snippet: 2-D PAGE: IPG dry strips were equilibrated for 12-16 hours with 7 M urea, thiourea containing 2% 3-[(3- Cholamidopropyl)dimethylammonio]-1-prpanesulfonate (CHAPS, Sigma), 1% dithiothreitol (DTT, Sigma), 1% pharmalyte in 200 μg samples. .. Isoelectric focusing (IEF) was performed at 20°C using a Article Title: Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil. Article Snippet: FK506 (tacrolimus) is a 23-membered polyketide macrolide that possesses powerful immunosuppressant activity.. In this study, feeding soybean oil into the fermentation culture of Streptomyces tsukubaensis improved FK506 production by 88.8%.. To decipher the overproduction mechanism, comparative proteomic and metabolomic analysis was carried out. Article Title: Identification of Proteins in Promastigote and Amastigote-like Leishmania Using an Immunoproteomic Approach Article Snippet: Next, samples were applied to IPG strips (13 cm, pH 4–7; GE Healthcare) for passive rehydration overnight at room temperature. .. After in-gel rehydration for 12 h, isoeletric focusing was performed at 500 V for 1 h, 1.000 V for 1 h, and 8.000 V for 8 h, using a Article Title: SPATC 1L maintains the integrity of the sperm head‐tail junction Article Snippet: .. For the first dimension of 2DE, a sample of the soluble fraction containing 200 μg protein was loaded onto the gel, followed by isoelectric focusing (IEF) at 20°C using a Article Title: Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis Article Snippet: For the first dimension, electrophoresis was applied onto IPG strips of 18 cm, pH=4–7 (Bio-Rad, Hercules, CA) rehydrated by loading the samples diluted with rehydration buffer (8M urea, 4% CHAPS, 2% ampholyte, 50 mM DTT, and traces of bromophenol blue) overnight. .. After the gel rehydration, IEF was performed at 300V/ 1h, 500V/ 1h, 1000V/ 2h, and 3500V/ 12h using a Article Title: Method of identifying abnormal cells by expression levels of ETFB Article Snippet: As the electrophoresis apparatus, Ettan IPGphor IEF System(Amersham) was used, and the conditions for development were as follows. .. 50 μA/Strip, 20° C. S1: Gradient 500 V, 1 min. S2: Gradient 4000 V, 4 h S3: Step-n-hold 8000 V, 10 h S4: Step-n-hold 6000 V, until recovery Thereafter, SDS-PAGE was carried out using Two-Dimensional Gel Electrophoresis:Article Title: SPATC 1L maintains the integrity of the sperm head‐tail junction Article Snippet: .. For the first dimension of 2DE, a sample of the soluble fraction containing 200 μg protein was loaded onto the gel, followed by isoelectric focusing (IEF) at 20°C using a SDS Page:Article Title: Method of identifying abnormal cells by expression levels of ETFB Article Snippet: As the electrophoresis apparatus, Ettan IPGphor IEF System(Amersham) was used, and the conditions for development were as follows. .. 50 μA/Strip, 20° C. S1: Gradient 500 V, 1 min. S2: Gradient 4000 V, 4 h S3: Step-n-hold 8000 V, 10 h S4: Step-n-hold 6000 V, until recovery Thereafter, SDS-PAGE was carried out using |