Incubation:Article Title: Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis.
Article Snippet: Sections were washed using a gentle stream of TBST buffer delivered using a squirt bottle to remove the majority of the primary antibody solution and placed in a coplin jar filled with TBST to wash on a nutating mixer (Fisher Scientific 88-861-041) for 30 min. .. The secondary antibody solution was added and sections were incubated in a black slide humidity chamber (FisherScientific NC9062083) for ten minutes. ..
Article Title: Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis
Article Snippet: Sections were washed using a gentle stream of TBST buffer delivered using a squirt bottle to remove the majority of the primary antibody solution and placed in a coplin jar filled with TBST to wash on a nutating mixer (Fisher Scientific 88–861-041) for 30 min. .. The secondary antibody solution was added and sections were incubated in a black slide humidity chamber (FisherScientific NC9062083) for ten minutes. ..
Tissue:Article Title: Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells
Article Snippet: 01-915-102, 01-917-142) Stirrer with ceramic plate, Isotemp 30°C to 540°C (Fisher Scientific, cat. no. FB30786163) Parafilm (Bemis, cat. no. PM996) Hamilton concept fume hood 0.22 μm filter (Millipore Sigma, cat. no. SLGPR33RS) BD Slip tip 1 mL sterile syringes (Fisher Scientific, cat. no. 14-823-434) CRITICAL For filtering HSA solutions during recombinant protein reconstitution. .. Cryostat (Leica, cat. no. 149491860us) Tissue-Tek biopsy cryomolds 10 mm × 10 mm × 5 mm (Sakura Finetek, cat. no. 4565) Tissue-Tek intermediate cryomolds 15 mm × 15 mm × 5 mm (Sakura Finetek, cat. no. 4566) Desiccator (Thermo Scientific, cat. no. 53110250) Superfrost Plus slides (Fisher Scientific, cat. no. 12-550-15) 24 mm x 50 mm #1 Thickness Cover Glass (Fisher Scientific, cat. no. 22-050-233) PAP pen (Electron Microscopy Sciences, cat. no. 71310) Coplin jar (IHC World LLC, cat. no. IW2501C) Humidified slide staining chamber (Fisher Scientific, cat. no. NC9062083) 3D platform rotator (Fisher Scientific, cat. no. 88-861-045) Eight-well silicone isolators 2.0 mm (Electron Microscopy Sciences, cat. no. 70339-44) Eight-well silicone isolators 2.5 mm (Electron Microscopy Sciences, cat. no. 70339-46) 12-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P12-1.5H-N) 24-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P24-1.5H-N) 96-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P96-1.5H-N) 35 mm Petri-dish with 14 mm diameter glass bottom microwell (MatTek, cat. no. P35G-1.5-14-C) Leica vibratome VT1000S and Leica vibratome VT1200 CRITICAL A separate vibratome for fixed and live tissue is recommended to avoid cross-contamination and accidental fixation of live tissue. .. Disposable Base Molds (Fisher Scientific, cat. no. 22-363-553) Disposable Transfer pipette (Thermo Scientific, cat. no. S304673) Vibratome sapphire blade (Delaware Diamond Knives, cat. no. SAFN) Minutien pins (Fine Science Tools, cat. no. 26002-10) 18 mm round coverslips (Thomas Scientific, cat. no. 1217N81) 12-well culture plate (Thermo Fisher Scientific, cat. no. 150628) 12-well glass-bottom plate (Cellvis, cat. no. P12-1.5H-N) Perforated metal spoon, diameter 20 mm (World Precision Instruments, cat. no. 501997) Nikon Ts2R (Nikon Instruments) Nikon Ti2 (Nikon Instruments) Nikon A1R (Nikon Instruments) NIS-Elements Imaging Software (Nikon Instruments) ImageJ Moria perforated spoon, diameter 15 mm (Fine Science Tools, cat. no. 1037018) Moria perforated spoon, diameter 20 mm (Fine Science Tools, cat. no. 10370-17) Kimwipes (Kimtech Science, cat. no. 34256) 0.6 mL snap cap low-retention microcentrifuge tubes (Thermo Scientific, cat. no. 3446) 1.5 mL snap cap low retention graduated microcentrifuge tubes (Thermo Scientific, cat. no. 3448) 2 mL round-bottom microcentrifuge tubes with locking snap cap (Fisher Scientific, cat. no. 14-666-315) 15 mL conical tubes (Thermo Scientific, cat. no. 12-565-268) 50 mL conical tubes (Thermo Scientific, cat. no. 12-565-270) 5 mL serological pipettes (Fisherbrand, cat. no. 13-678-11D) 10 mL serological pipettes (Fisherbrand, cat. no. 13-678-11E) 25 mL serological pipettes (Fisherbrand, cat. no. 13-678-11) 50 mL serological pipettes (Fisherbrand, cat. no. 13-678-11F) Starter Kit including 4 LTS Pipet-Lite XLS+ manual single channel pipettes (Rainin, cat. no. 30386597) 20 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389229) 250 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389246) 250 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin, cat. no. 30389250) 1,000 μL LTS pipette tips, low retention, sterile (Rainin cat. no. 30389216) 1,000 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin cat. no. 30389221) For details on the reconstitution of human recombinant bFGF, human recombinant BMP4, STEMCELL Technologies BMP4, SB and LDN in solution, as well as preparations of E8 Flex medium, E6 medium, E8 containing Y-27632 have been previously described.
Glass:Article Title: Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells
Article Snippet: 01-915-102, 01-917-142) Stirrer with ceramic plate, Isotemp 30°C to 540°C (Fisher Scientific, cat. no. FB30786163) Parafilm (Bemis, cat. no. PM996) Hamilton concept fume hood 0.22 μm filter (Millipore Sigma, cat. no. SLGPR33RS) BD Slip tip 1 mL sterile syringes (Fisher Scientific, cat. no. 14-823-434) CRITICAL For filtering HSA solutions during recombinant protein reconstitution. .. Cryostat (Leica, cat. no. 149491860us) Tissue-Tek biopsy cryomolds 10 mm × 10 mm × 5 mm (Sakura Finetek, cat. no. 4565) Tissue-Tek intermediate cryomolds 15 mm × 15 mm × 5 mm (Sakura Finetek, cat. no. 4566) Desiccator (Thermo Scientific, cat. no. 53110250) Superfrost Plus slides (Fisher Scientific, cat. no. 12-550-15) 24 mm x 50 mm #1 Thickness Cover Glass (Fisher Scientific, cat. no. 22-050-233) PAP pen (Electron Microscopy Sciences, cat. no. 71310) Coplin jar (IHC World LLC, cat. no. IW2501C) Humidified slide staining chamber (Fisher Scientific, cat. no. NC9062083) 3D platform rotator (Fisher Scientific, cat. no. 88-861-045) Eight-well silicone isolators 2.0 mm (Electron Microscopy Sciences, cat. no. 70339-44) Eight-well silicone isolators 2.5 mm (Electron Microscopy Sciences, cat. no. 70339-46) 12-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P12-1.5H-N) 24-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P24-1.5H-N) 96-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P96-1.5H-N) 35 mm Petri-dish with 14 mm diameter glass bottom microwell (MatTek, cat. no. P35G-1.5-14-C) Leica vibratome VT1000S and Leica vibratome VT1200 CRITICAL A separate vibratome for fixed and live tissue is recommended to avoid cross-contamination and accidental fixation of live tissue. .. Disposable Base Molds (Fisher Scientific, cat. no. 22-363-553) Disposable Transfer pipette (Thermo Scientific, cat. no. S304673) Vibratome sapphire blade (Delaware Diamond Knives, cat. no. SAFN) Minutien pins (Fine Science Tools, cat. no. 26002-10) 18 mm round coverslips (Thomas Scientific, cat. no. 1217N81) 12-well culture plate (Thermo Fisher Scientific, cat. no. 150628) 12-well glass-bottom plate (Cellvis, cat. no. P12-1.5H-N) Perforated metal spoon, diameter 20 mm (World Precision Instruments, cat. no. 501997) Nikon Ts2R (Nikon Instruments) Nikon Ti2 (Nikon Instruments) Nikon A1R (Nikon Instruments) NIS-Elements Imaging Software (Nikon Instruments) ImageJ Moria perforated spoon, diameter 15 mm (Fine Science Tools, cat. no. 1037018) Moria perforated spoon, diameter 20 mm (Fine Science Tools, cat. no. 10370-17) Kimwipes (Kimtech Science, cat. no. 34256) 0.6 mL snap cap low-retention microcentrifuge tubes (Thermo Scientific, cat. no. 3446) 1.5 mL snap cap low retention graduated microcentrifuge tubes (Thermo Scientific, cat. no. 3448) 2 mL round-bottom microcentrifuge tubes with locking snap cap (Fisher Scientific, cat. no. 14-666-315) 15 mL conical tubes (Thermo Scientific, cat. no. 12-565-268) 50 mL conical tubes (Thermo Scientific, cat. no. 12-565-270) 5 mL serological pipettes (Fisherbrand, cat. no. 13-678-11D) 10 mL serological pipettes (Fisherbrand, cat. no. 13-678-11E) 25 mL serological pipettes (Fisherbrand, cat. no. 13-678-11) 50 mL serological pipettes (Fisherbrand, cat. no. 13-678-11F) Starter Kit including 4 LTS Pipet-Lite XLS+ manual single channel pipettes (Rainin, cat. no. 30386597) 20 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389229) 250 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389246) 250 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin, cat. no. 30389250) 1,000 μL LTS pipette tips, low retention, sterile (Rainin cat. no. 30389216) 1,000 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin cat. no. 30389221) For details on the reconstitution of human recombinant bFGF, human recombinant BMP4, STEMCELL Technologies BMP4, SB and LDN in solution, as well as preparations of E8 Flex medium, E6 medium, E8 containing Y-27632 have been previously described.
Electron Microscopy:Article Title: Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells
Article Snippet: 01-915-102, 01-917-142) Stirrer with ceramic plate, Isotemp 30°C to 540°C (Fisher Scientific, cat. no. FB30786163) Parafilm (Bemis, cat. no. PM996) Hamilton concept fume hood 0.22 μm filter (Millipore Sigma, cat. no. SLGPR33RS) BD Slip tip 1 mL sterile syringes (Fisher Scientific, cat. no. 14-823-434) CRITICAL For filtering HSA solutions during recombinant protein reconstitution. .. Cryostat (Leica, cat. no. 149491860us) Tissue-Tek biopsy cryomolds 10 mm × 10 mm × 5 mm (Sakura Finetek, cat. no. 4565) Tissue-Tek intermediate cryomolds 15 mm × 15 mm × 5 mm (Sakura Finetek, cat. no. 4566) Desiccator (Thermo Scientific, cat. no. 53110250) Superfrost Plus slides (Fisher Scientific, cat. no. 12-550-15) 24 mm x 50 mm #1 Thickness Cover Glass (Fisher Scientific, cat. no. 22-050-233) PAP pen (Electron Microscopy Sciences, cat. no. 71310) Coplin jar (IHC World LLC, cat. no. IW2501C) Humidified slide staining chamber (Fisher Scientific, cat. no. NC9062083) 3D platform rotator (Fisher Scientific, cat. no. 88-861-045) Eight-well silicone isolators 2.0 mm (Electron Microscopy Sciences, cat. no. 70339-44) Eight-well silicone isolators 2.5 mm (Electron Microscopy Sciences, cat. no. 70339-46) 12-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P12-1.5H-N) 24-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P24-1.5H-N) 96-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P96-1.5H-N) 35 mm Petri-dish with 14 mm diameter glass bottom microwell (MatTek, cat. no. P35G-1.5-14-C) Leica vibratome VT1000S and Leica vibratome VT1200 CRITICAL A separate vibratome for fixed and live tissue is recommended to avoid cross-contamination and accidental fixation of live tissue. .. Disposable Base Molds (Fisher Scientific, cat. no. 22-363-553) Disposable Transfer pipette (Thermo Scientific, cat. no. S304673) Vibratome sapphire blade (Delaware Diamond Knives, cat. no. SAFN) Minutien pins (Fine Science Tools, cat. no. 26002-10) 18 mm round coverslips (Thomas Scientific, cat. no. 1217N81) 12-well culture plate (Thermo Fisher Scientific, cat. no. 150628) 12-well glass-bottom plate (Cellvis, cat. no. P12-1.5H-N) Perforated metal spoon, diameter 20 mm (World Precision Instruments, cat. no. 501997) Nikon Ts2R (Nikon Instruments) Nikon Ti2 (Nikon Instruments) Nikon A1R (Nikon Instruments) NIS-Elements Imaging Software (Nikon Instruments) ImageJ Moria perforated spoon, diameter 15 mm (Fine Science Tools, cat. no. 1037018) Moria perforated spoon, diameter 20 mm (Fine Science Tools, cat. no. 10370-17) Kimwipes (Kimtech Science, cat. no. 34256) 0.6 mL snap cap low-retention microcentrifuge tubes (Thermo Scientific, cat. no. 3446) 1.5 mL snap cap low retention graduated microcentrifuge tubes (Thermo Scientific, cat. no. 3448) 2 mL round-bottom microcentrifuge tubes with locking snap cap (Fisher Scientific, cat. no. 14-666-315) 15 mL conical tubes (Thermo Scientific, cat. no. 12-565-268) 50 mL conical tubes (Thermo Scientific, cat. no. 12-565-270) 5 mL serological pipettes (Fisherbrand, cat. no. 13-678-11D) 10 mL serological pipettes (Fisherbrand, cat. no. 13-678-11E) 25 mL serological pipettes (Fisherbrand, cat. no. 13-678-11) 50 mL serological pipettes (Fisherbrand, cat. no. 13-678-11F) Starter Kit including 4 LTS Pipet-Lite XLS+ manual single channel pipettes (Rainin, cat. no. 30386597) 20 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389229) 250 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389246) 250 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin, cat. no. 30389250) 1,000 μL LTS pipette tips, low retention, sterile (Rainin cat. no. 30389216) 1,000 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin cat. no. 30389221) For details on the reconstitution of human recombinant bFGF, human recombinant BMP4, STEMCELL Technologies BMP4, SB and LDN in solution, as well as preparations of E8 Flex medium, E6 medium, E8 containing Y-27632 have been previously described.
Staining:Article Title: Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells
Article Snippet: 01-915-102, 01-917-142) Stirrer with ceramic plate, Isotemp 30°C to 540°C (Fisher Scientific, cat. no. FB30786163) Parafilm (Bemis, cat. no. PM996) Hamilton concept fume hood 0.22 μm filter (Millipore Sigma, cat. no. SLGPR33RS) BD Slip tip 1 mL sterile syringes (Fisher Scientific, cat. no. 14-823-434) CRITICAL For filtering HSA solutions during recombinant protein reconstitution. .. Cryostat (Leica, cat. no. 149491860us) Tissue-Tek biopsy cryomolds 10 mm × 10 mm × 5 mm (Sakura Finetek, cat. no. 4565) Tissue-Tek intermediate cryomolds 15 mm × 15 mm × 5 mm (Sakura Finetek, cat. no. 4566) Desiccator (Thermo Scientific, cat. no. 53110250) Superfrost Plus slides (Fisher Scientific, cat. no. 12-550-15) 24 mm x 50 mm #1 Thickness Cover Glass (Fisher Scientific, cat. no. 22-050-233) PAP pen (Electron Microscopy Sciences, cat. no. 71310) Coplin jar (IHC World LLC, cat. no. IW2501C) Humidified slide staining chamber (Fisher Scientific, cat. no. NC9062083) 3D platform rotator (Fisher Scientific, cat. no. 88-861-045) Eight-well silicone isolators 2.0 mm (Electron Microscopy Sciences, cat. no. 70339-44) Eight-well silicone isolators 2.5 mm (Electron Microscopy Sciences, cat. no. 70339-46) 12-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P12-1.5H-N) 24-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P24-1.5H-N) 96-well glass bottom plate with high performance #1.5 cover glass (Cellvis, cat no. P96-1.5H-N) 35 mm Petri-dish with 14 mm diameter glass bottom microwell (MatTek, cat. no. P35G-1.5-14-C) Leica vibratome VT1000S and Leica vibratome VT1200 CRITICAL A separate vibratome for fixed and live tissue is recommended to avoid cross-contamination and accidental fixation of live tissue. .. Disposable Base Molds (Fisher Scientific, cat. no. 22-363-553) Disposable Transfer pipette (Thermo Scientific, cat. no. S304673) Vibratome sapphire blade (Delaware Diamond Knives, cat. no. SAFN) Minutien pins (Fine Science Tools, cat. no. 26002-10) 18 mm round coverslips (Thomas Scientific, cat. no. 1217N81) 12-well culture plate (Thermo Fisher Scientific, cat. no. 150628) 12-well glass-bottom plate (Cellvis, cat. no. P12-1.5H-N) Perforated metal spoon, diameter 20 mm (World Precision Instruments, cat. no. 501997) Nikon Ts2R (Nikon Instruments) Nikon Ti2 (Nikon Instruments) Nikon A1R (Nikon Instruments) NIS-Elements Imaging Software (Nikon Instruments) ImageJ Moria perforated spoon, diameter 15 mm (Fine Science Tools, cat. no. 1037018) Moria perforated spoon, diameter 20 mm (Fine Science Tools, cat. no. 10370-17) Kimwipes (Kimtech Science, cat. no. 34256) 0.6 mL snap cap low-retention microcentrifuge tubes (Thermo Scientific, cat. no. 3446) 1.5 mL snap cap low retention graduated microcentrifuge tubes (Thermo Scientific, cat. no. 3448) 2 mL round-bottom microcentrifuge tubes with locking snap cap (Fisher Scientific, cat. no. 14-666-315) 15 mL conical tubes (Thermo Scientific, cat. no. 12-565-268) 50 mL conical tubes (Thermo Scientific, cat. no. 12-565-270) 5 mL serological pipettes (Fisherbrand, cat. no. 13-678-11D) 10 mL serological pipettes (Fisherbrand, cat. no. 13-678-11E) 25 mL serological pipettes (Fisherbrand, cat. no. 13-678-11) 50 mL serological pipettes (Fisherbrand, cat. no. 13-678-11F) Starter Kit including 4 LTS Pipet-Lite XLS+ manual single channel pipettes (Rainin, cat. no. 30386597) 20 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389229) 250 μL LTS pipette tips, low retention, sterile (Rainin, cat. no. 30389246) 250 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin, cat. no. 30389250) 1,000 μL LTS pipette tips, low retention, sterile (Rainin cat. no. 30389216) 1,000 μL LTS pipette tips, wide orifice, low retention, sterile (Rainin cat. no. 30389221) For details on the reconstitution of human recombinant bFGF, human recombinant BMP4, STEMCELL Technologies BMP4, SB and LDN in solution, as well as preparations of E8 Flex medium, E6 medium, E8 containing Y-27632 have been previously described.
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